Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm

This work aimed to evaluate the effect of crocin on frozen-thawed sperm quality in buffalo. Spermatozoa were incubated in Tyrode’s Albumin Lactate Pyruvate medium supplemented with 0, 0.5, 1, and 2 mM crocin for 2 h. Sperm motility was evaluated by phase-contrast microscopy, viability and acrosome i...

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Autores principales:	Valentina Longobardi, Giovanni della Valle, Francesco Iannaccone, Alfonso Calabria, Gabriele Di Vuolo, Sara Damiano, Roberto Ciarcia, Bianca Gasparrini
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	buffalo sperm crocin oxidative stress Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/1c759694df9b4c399c217ff21e4f38da
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spelling	oai:doaj.org-article:1c759694df9b4c399c217ff21e4f38da2021-11-11T14:23:42ZEffects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm1594-40771828-051X10.1080/1828051X.2021.1997653https://doaj.org/article/1c759694df9b4c399c217ff21e4f38da2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/1828051X.2021.1997653https://doaj.org/toc/1594-4077https://doaj.org/toc/1828-051XThis work aimed to evaluate the effect of crocin on frozen-thawed sperm quality in buffalo. Spermatozoa were incubated in Tyrode’s Albumin Lactate Pyruvate medium supplemented with 0, 0.5, 1, and 2 mM crocin for 2 h. Sperm motility was evaluated by phase-contrast microscopy, viability and acrosome integrity by Trypan blue Giemsa staining, and membrane functional integrity by the hypoosmotic swelling test. The DNA fragmentation was evaluated by Tunel and ROS levels by spectrofluorometric analysis. The treatment with 2 mM of crocin increased (P < .05) sperm membrane functional integrity compared to the control group (59.1 ± 1.6 vs 53.3 ± 1.5) and reduced sperm DNA fragmentation, compared to the other groups (11.3 ± 1.1, 13.3 ± 1.2, 13.6 ± 1.2 and 6.0 ± 0.7, respectively in 0, 0.5, 1 and 2 mM crocin; P < .01). Finally, a dose-dependent decrease (P < .01) in superoxide anion production in the presence of crocin was observed, as indicated by Dihydroethidium values (922.6 ± 13.0, 596.8 ± 7.4, 498.9 ± 5.3 and 421.4 ± 5.0 a.u., respectively in 0, 0.5, 1 and 2 mM crocin; P < .01). The results of this study demonstrated a positive effect of 2 mM crocin on frozen-thawed buffalo sperm, as indicated by the improvement of sperm membrane integrity and the reduction of DNA fragmentation and ROS levels.Highlights Crocin improves buffalo sperm quality. Crocin improves sperm membrane integrity and reduces DNA fragmentation. Crocin decreases oxidative stress in buffalo sperm.Valentina LongobardiGiovanni della ValleFrancesco IannacconeAlfonso CalabriaGabriele Di VuoloSara DamianoRoberto CiarciaBianca GasparriniTaylor & Francis Grouparticlebuffalospermcrocinoxidative stressAnimal cultureSF1-1100ENItalian Journal of Animal Science, Vol 20, Iss 1, Pp 2095-2101 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	buffalo sperm crocin oxidative stress Animal culture SF1-1100
spellingShingle	buffalo sperm crocin oxidative stress Animal culture SF1-1100 Valentina Longobardi Giovanni della Valle Francesco Iannaccone Alfonso Calabria Gabriele Di Vuolo Sara Damiano Roberto Ciarcia Bianca Gasparrini Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm
description	This work aimed to evaluate the effect of crocin on frozen-thawed sperm quality in buffalo. Spermatozoa were incubated in Tyrode’s Albumin Lactate Pyruvate medium supplemented with 0, 0.5, 1, and 2 mM crocin for 2 h. Sperm motility was evaluated by phase-contrast microscopy, viability and acrosome integrity by Trypan blue Giemsa staining, and membrane functional integrity by the hypoosmotic swelling test. The DNA fragmentation was evaluated by Tunel and ROS levels by spectrofluorometric analysis. The treatment with 2 mM of crocin increased (P < .05) sperm membrane functional integrity compared to the control group (59.1 ± 1.6 vs 53.3 ± 1.5) and reduced sperm DNA fragmentation, compared to the other groups (11.3 ± 1.1, 13.3 ± 1.2, 13.6 ± 1.2 and 6.0 ± 0.7, respectively in 0, 0.5, 1 and 2 mM crocin; P < .01). Finally, a dose-dependent decrease (P < .01) in superoxide anion production in the presence of crocin was observed, as indicated by Dihydroethidium values (922.6 ± 13.0, 596.8 ± 7.4, 498.9 ± 5.3 and 421.4 ± 5.0 a.u., respectively in 0, 0.5, 1 and 2 mM crocin; P < .01). The results of this study demonstrated a positive effect of 2 mM crocin on frozen-thawed buffalo sperm, as indicated by the improvement of sperm membrane integrity and the reduction of DNA fragmentation and ROS levels.Highlights Crocin improves buffalo sperm quality. Crocin improves sperm membrane integrity and reduces DNA fragmentation. Crocin decreases oxidative stress in buffalo sperm.
format	article
author	Valentina Longobardi Giovanni della Valle Francesco Iannaccone Alfonso Calabria Gabriele Di Vuolo Sara Damiano Roberto Ciarcia Bianca Gasparrini
author_facet	Valentina Longobardi Giovanni della Valle Francesco Iannaccone Alfonso Calabria Gabriele Di Vuolo Sara Damiano Roberto Ciarcia Bianca Gasparrini
author_sort	Valentina Longobardi
title	Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm
title_short	Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm
title_full	Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm
title_fullStr	Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm
title_full_unstemmed	Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm
title_sort	effects of the antioxidant crocin on frozen-thawed buffalo (bubalus bubalis) sperm
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/1c759694df9b4c399c217ff21e4f38da
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Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalus bubalis) sperm

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