Comparison of Different Drying Methods for Recovery of Mushroom DNA

Abstract Several methods have been reported for drying mushroom specimens for population genetic, taxonomic, and phylogenetic studies. However, most methods have not been directly compared for their effectiveness in preserving mushroom DNA. In this study, we compared silica gel drying at ambient tem...

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Autores principales:	Shouxian Wang, Yu Liu, Jianping Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1c782347cc5d4f5a968c6387af963ec4
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spelling	oai:doaj.org-article:1c782347cc5d4f5a968c6387af963ec42021-12-02T11:53:06ZComparison of Different Drying Methods for Recovery of Mushroom DNA10.1038/s41598-017-03570-72045-2322https://doaj.org/article/1c782347cc5d4f5a968c6387af963ec42017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03570-7https://doaj.org/toc/2045-2322Abstract Several methods have been reported for drying mushroom specimens for population genetic, taxonomic, and phylogenetic studies. However, most methods have not been directly compared for their effectiveness in preserving mushroom DNA. In this study, we compared silica gel drying at ambient temperature and oven drying at seven different temperatures. Two mushroom species representing two types of fruiting bodies were examined: the fleshy button mushroom Agaricus bisporus and the leathery shelf fungus Trametes versicolor. For each species dried with the eight methods, we assessed the mushroom water loss rate, the quality and quantity of extracted DNA, and the effectiveness of using the extracted DNA as a template for PCR amplification of two DNA fragments (ITS and a single copy gene). Dried specimens from all tested methods yielded sufficient DNA for PCR amplification of the two genes in both species. However, differences among the methods for the two species were found in: (i) the time required by different drying methods for the fresh mushroom tissue to reach a stable weight; and (ii) the relative quality and quantity of the extracted genomic DNA. Among these methods, oven drying at 70 °C for 3–4 h seemed the most efficient for preserving field mushroom samples for subsequent molecular work.Shouxian WangYu LiuJianping XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shouxian Wang Yu Liu Jianping Xu Comparison of Different Drying Methods for Recovery of Mushroom DNA
description	Abstract Several methods have been reported for drying mushroom specimens for population genetic, taxonomic, and phylogenetic studies. However, most methods have not been directly compared for their effectiveness in preserving mushroom DNA. In this study, we compared silica gel drying at ambient temperature and oven drying at seven different temperatures. Two mushroom species representing two types of fruiting bodies were examined: the fleshy button mushroom Agaricus bisporus and the leathery shelf fungus Trametes versicolor. For each species dried with the eight methods, we assessed the mushroom water loss rate, the quality and quantity of extracted DNA, and the effectiveness of using the extracted DNA as a template for PCR amplification of two DNA fragments (ITS and a single copy gene). Dried specimens from all tested methods yielded sufficient DNA for PCR amplification of the two genes in both species. However, differences among the methods for the two species were found in: (i) the time required by different drying methods for the fresh mushroom tissue to reach a stable weight; and (ii) the relative quality and quantity of the extracted genomic DNA. Among these methods, oven drying at 70 °C for 3–4 h seemed the most efficient for preserving field mushroom samples for subsequent molecular work.
format	article
author	Shouxian Wang Yu Liu Jianping Xu
author_facet	Shouxian Wang Yu Liu Jianping Xu
author_sort	Shouxian Wang
title	Comparison of Different Drying Methods for Recovery of Mushroom DNA
title_short	Comparison of Different Drying Methods for Recovery of Mushroom DNA
title_full	Comparison of Different Drying Methods for Recovery of Mushroom DNA
title_fullStr	Comparison of Different Drying Methods for Recovery of Mushroom DNA
title_full_unstemmed	Comparison of Different Drying Methods for Recovery of Mushroom DNA
title_sort	comparison of different drying methods for recovery of mushroom dna
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1c782347cc5d4f5a968c6387af963ec4
work_keys_str_mv	AT shouxianwang comparisonofdifferentdryingmethodsforrecoveryofmushroomdna AT yuliu comparisonofdifferentdryingmethodsforrecoveryofmushroomdna AT jianpingxu comparisonofdifferentdryingmethodsforrecoveryofmushroomdna
_version_	1718394853044256768

Comparison of Different Drying Methods for Recovery of Mushroom DNA

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