Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo

Abstract Royal jelly (RJ) has successfully been used as a remedy in wound healing. RJ has multiple effects, including antibacterial, anti-inflammatory and immunomodulatory activities, in various cell types. However, no component(s) (other than antibacterial) have been identified in RJ-accelerated wo...

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Autores principales:	Marcela Bucekova, Martin Sojka, Ivana Valachova, Simona Martinotti, Elia Ranzato, Zoltan Szep, Viktor Majtan, Jaroslav Klaudiny, Juraj Majtan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1c7a8516611446e8917646b678e57ca2
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spelling	oai:doaj.org-article:1c7a8516611446e8917646b678e57ca22021-12-02T12:32:18ZBee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo10.1038/s41598-017-07494-02045-2322https://doaj.org/article/1c7a8516611446e8917646b678e57ca22017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07494-0https://doaj.org/toc/2045-2322Abstract Royal jelly (RJ) has successfully been used as a remedy in wound healing. RJ has multiple effects, including antibacterial, anti-inflammatory and immunomodulatory activities, in various cell types. However, no component(s) (other than antibacterial) have been identified in RJ-accelerated wound healing. In this study, we demonstrate that keratinocytes are responsible for the elevated production of matrix metalloproteinase-9 (MMP-9) after incubation with a water extract of RJ. Furthermore, the keratinocyte migration and wound closure rates were significantly increased in the presence of RJ extract. MMP-9 production was reduced significantly following proteinase K treatment but remained stable after heat treatment, indicating that active component(s) have a proteinous character. To identify the component responsible for inducing MMP-9 production, RJ extract was fractionated using C18 RP-HPLC. In fractions exhibiting stimulatory activity, we immunochemically detected the bee-derived antibacterial peptide, defensin-1. Defensin-1 was cloned, and recombinant peptide was produced in a baculoviral expression system. Defensin-1 stimulated MMP-9 secretion from keratinocytes and increased keratinocyte migration and wound closure in vitro. In addition, defensin-1 promoted re-epithelisation and wound closure in uninfected excision wounds. These data indisputably demonstrate that defensin-1, a regular but concentration variable factor found in honey and RJ, contributes to cutaneous wound closure by enhancing keratinocyte migration and MMP-9 secretion.Marcela BucekovaMartin SojkaIvana ValachovaSimona MartinottiElia RanzatoZoltan SzepViktor MajtanJaroslav KlaudinyJuraj MajtanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcela Bucekova Martin Sojka Ivana Valachova Simona Martinotti Elia Ranzato Zoltan Szep Viktor Majtan Jaroslav Klaudiny Juraj Majtan Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
description	Abstract Royal jelly (RJ) has successfully been used as a remedy in wound healing. RJ has multiple effects, including antibacterial, anti-inflammatory and immunomodulatory activities, in various cell types. However, no component(s) (other than antibacterial) have been identified in RJ-accelerated wound healing. In this study, we demonstrate that keratinocytes are responsible for the elevated production of matrix metalloproteinase-9 (MMP-9) after incubation with a water extract of RJ. Furthermore, the keratinocyte migration and wound closure rates were significantly increased in the presence of RJ extract. MMP-9 production was reduced significantly following proteinase K treatment but remained stable after heat treatment, indicating that active component(s) have a proteinous character. To identify the component responsible for inducing MMP-9 production, RJ extract was fractionated using C18 RP-HPLC. In fractions exhibiting stimulatory activity, we immunochemically detected the bee-derived antibacterial peptide, defensin-1. Defensin-1 was cloned, and recombinant peptide was produced in a baculoviral expression system. Defensin-1 stimulated MMP-9 secretion from keratinocytes and increased keratinocyte migration and wound closure in vitro. In addition, defensin-1 promoted re-epithelisation and wound closure in uninfected excision wounds. These data indisputably demonstrate that defensin-1, a regular but concentration variable factor found in honey and RJ, contributes to cutaneous wound closure by enhancing keratinocyte migration and MMP-9 secretion.
format	article
author	Marcela Bucekova Martin Sojka Ivana Valachova Simona Martinotti Elia Ranzato Zoltan Szep Viktor Majtan Jaroslav Klaudiny Juraj Majtan
author_facet	Marcela Bucekova Martin Sojka Ivana Valachova Simona Martinotti Elia Ranzato Zoltan Szep Viktor Majtan Jaroslav Klaudiny Juraj Majtan
author_sort	Marcela Bucekova
title	Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
title_short	Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
title_full	Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
title_fullStr	Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
title_full_unstemmed	Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
title_sort	bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1c7a8516611446e8917646b678e57ca2
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_version_	1718394081961312256

Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo

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