The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content>
ABSTRACT Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of m...
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American Society for Microbiology
2020
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oai:doaj.org-article:1c7b862b9b994dc2a5e97af9c858d8fb2021-11-15T15:30:51ZThe H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content>10.1128/mSphere.00793-202379-5042https://doaj.org/article/1c7b862b9b994dc2a5e97af9c858d8fb2020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00793-20https://doaj.org/toc/2379-5042ABSTRACT Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), known to elicit envelope stress by the accumulation of toxic precursors in the periplasm, to interrogate the role of H-NS in Acinetobacter baumannii, together with other stressors. Using a multidrug-resistant A. baumannii strain, we observed that H-NS plays a role in alleviating the stress triggered by MBL toxic precursors and counteracts the effect of DNA-damaging agents, supporting its role in stress response. IMPORTANCE Carbapenem-resistant A. baumannii (CRAB) is recognized as one of the most threatening Gram-negative bacilli. H-NS is known to play a role in controlling the transcription of a variety of different genes, including those associated with the stress response, persistence, and virulence. In the present work, we uncovered a link between the role of H-NS in the A. baumannii stress response and its relationship with the envelope stress response and resistance to DNA-damaging agents. Overall, we posit a new role of H-NS, showing that H-NS serves to endure envelope stress and could also be a mechanism that alleviates the stress induced by MBL expression in A. baumannii. This could be an evolutionary advantage to further resist the action of carbapenems.Fanny HuangNoelle FitchettChelsea Razo-GutierrezCasin LeJasmine MartinezGrace RaCarolina LopezLisandro J. GonzalezRodrigo SieiraAlejandro J. VilaRobert A. BonomoMaria Soledad RamirezAmerican Society for MicrobiologyarticleAcinetobacter baumanniiH-NSstresscarbapenemasesAcinetobactercarbapenemsMicrobiologyQR1-502ENmSphere, Vol 5, Iss 4 (2020) |
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DOAJ |
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DOAJ |
| language |
EN |
| topic |
Acinetobacter baumannii H-NS stress carbapenemases Acinetobacter carbapenems Microbiology QR1-502 |
| spellingShingle |
Acinetobacter baumannii H-NS stress carbapenemases Acinetobacter carbapenems Microbiology QR1-502 Fanny Huang Noelle Fitchett Chelsea Razo-Gutierrez Casin Le Jasmine Martinez Grace Ra Carolina Lopez Lisandro J. Gonzalez Rodrigo Sieira Alejandro J. Vila Robert A. Bonomo Maria Soledad Ramirez The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| description |
ABSTRACT Disruption of the histone-like nucleoid structuring protein (H-NS) was shown to affect the ability of Gram-negative bacteria to regulate genes associated with virulence, persistence, stress response, quorum sensing, biosynthesis pathways, and cell adhesion. Here, we used the expression of metallo-β-lactamases (MBLs), known to elicit envelope stress by the accumulation of toxic precursors in the periplasm, to interrogate the role of H-NS in Acinetobacter baumannii, together with other stressors. Using a multidrug-resistant A. baumannii strain, we observed that H-NS plays a role in alleviating the stress triggered by MBL toxic precursors and counteracts the effect of DNA-damaging agents, supporting its role in stress response. IMPORTANCE Carbapenem-resistant A. baumannii (CRAB) is recognized as one of the most threatening Gram-negative bacilli. H-NS is known to play a role in controlling the transcription of a variety of different genes, including those associated with the stress response, persistence, and virulence. In the present work, we uncovered a link between the role of H-NS in the A. baumannii stress response and its relationship with the envelope stress response and resistance to DNA-damaging agents. Overall, we posit a new role of H-NS, showing that H-NS serves to endure envelope stress and could also be a mechanism that alleviates the stress induced by MBL expression in A. baumannii. This could be an evolutionary advantage to further resist the action of carbapenems. |
| format |
article |
| author |
Fanny Huang Noelle Fitchett Chelsea Razo-Gutierrez Casin Le Jasmine Martinez Grace Ra Carolina Lopez Lisandro J. Gonzalez Rodrigo Sieira Alejandro J. Vila Robert A. Bonomo Maria Soledad Ramirez |
| author_facet |
Fanny Huang Noelle Fitchett Chelsea Razo-Gutierrez Casin Le Jasmine Martinez Grace Ra Carolina Lopez Lisandro J. Gonzalez Rodrigo Sieira Alejandro J. Vila Robert A. Bonomo Maria Soledad Ramirez |
| author_sort |
Fanny Huang |
| title |
The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_short |
The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_full |
The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_fullStr |
The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_full_unstemmed |
The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in <named-content content-type="genus-species">Acinetobacter baumannii</named-content> |
| title_sort |
h-ns regulator plays a role in the stress induced by carbapenemase expression in <named-content content-type="genus-species">acinetobacter baumannii</named-content> |
| publisher |
American Society for Microbiology |
| publishDate |
2020 |
| url |
https://doaj.org/article/1c7b862b9b994dc2a5e97af9c858d8fb |
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