A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced

Abstract. Zilda DS, Yulianti Y, Sholihah RF, Subaryono S, Fawzya YN, Irianto HE.2019. A novel Bacillus sp. isolated from rotten seaweed: identification and characterization alginate lyase its produced. Biodiversitas 20: 1166-1172. Alginate lyase has been known as potential biocatalyst not only for i...

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Autores principales:	DEWI SESWITA ZILDA, YULIYANTI YULIANTI, RIZKY FAUZIYAH SHOLIHAH, SUBARYONO SUBARYONO, YUSRO NURI FAWZYA, HARI EKO IRIANTO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	alginate lyase, algt513, bacillus sp., rotten seaweed Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1c7f09fb109b42a9b89d4433f64fce69
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spelling	oai:doaj.org-article:1c7f09fb109b42a9b89d4433f64fce692021-11-21T21:40:39ZA novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced1412-033X2085-472210.13057/biodiv/d200432https://doaj.org/article/1c7f09fb109b42a9b89d4433f64fce692019-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2378https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Zilda DS, Yulianti Y, Sholihah RF, Subaryono S, Fawzya YN, Irianto HE.2019. A novel Bacillus sp. isolated from rotten seaweed: identification and characterization alginate lyase its produced. Biodiversitas 20: 1166-1172. Alginate lyase has been known as potential biocatalyst not only for industrial but also medicinal application especially for the production of oligosaccharides which have distinct bioactivities. An alginate lyase, AlgT513, has been isolated from rotten seaweed bacterium strain T513 and characterized. The bacterium showed low similarity (95%) with Bacillus tequilensis strain 10b based on 16S rDNA sequence indicating that AlgT513may be a novel Bacillus species. The bacterium forms a clear zone on solid medium with 0.5% sodium alginate addition. The optimum temperature and pH were 50ºC and 8 respectively. AlgT513 maintained stability at board pHs of 4-9 and temperature of 45ºC. Metal ions Mg2+, Ca2+ and K+ increase the activity of the enzyme while Zn2+, Co2+ and Li+ strongly inhibit it. NaCl inhibits AlgT513 activity where most of the alginate lyases need it to reach maximum activity. AlgT513 is suggested as a serine metalloenzyme due to inhibition of ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).DEWI SESWITA ZILDAYULIYANTI YULIANTIRIZKY FAUZIYAH SHOLIHAHSUBARYONO SUBARYONOYUSRO NURI FAWZYAHARI EKO IRIANTOMBI & UNS Soloarticlealginate lyase, algt513, bacillus sp., rotten seaweedBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 4, Pp 1166-1172 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	alginate lyase, algt513, bacillus sp., rotten seaweed Biology (General) QH301-705.5
spellingShingle	alginate lyase, algt513, bacillus sp., rotten seaweed Biology (General) QH301-705.5 DEWI SESWITA ZILDA YULIYANTI YULIANTI RIZKY FAUZIYAH SHOLIHAH SUBARYONO SUBARYONO YUSRO NURI FAWZYA HARI EKO IRIANTO A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced
description	Abstract. Zilda DS, Yulianti Y, Sholihah RF, Subaryono S, Fawzya YN, Irianto HE.2019. A novel Bacillus sp. isolated from rotten seaweed: identification and characterization alginate lyase its produced. Biodiversitas 20: 1166-1172. Alginate lyase has been known as potential biocatalyst not only for industrial but also medicinal application especially for the production of oligosaccharides which have distinct bioactivities. An alginate lyase, AlgT513, has been isolated from rotten seaweed bacterium strain T513 and characterized. The bacterium showed low similarity (95%) with Bacillus tequilensis strain 10b based on 16S rDNA sequence indicating that AlgT513may be a novel Bacillus species. The bacterium forms a clear zone on solid medium with 0.5% sodium alginate addition. The optimum temperature and pH were 50ºC and 8 respectively. AlgT513 maintained stability at board pHs of 4-9 and temperature of 45ºC. Metal ions Mg2+, Ca2+ and K+ increase the activity of the enzyme while Zn2+, Co2+ and Li+ strongly inhibit it. NaCl inhibits AlgT513 activity where most of the alginate lyases need it to reach maximum activity. AlgT513 is suggested as a serine metalloenzyme due to inhibition of ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).
format	article
author	DEWI SESWITA ZILDA YULIYANTI YULIANTI RIZKY FAUZIYAH SHOLIHAH SUBARYONO SUBARYONO YUSRO NURI FAWZYA HARI EKO IRIANTO
author_facet	DEWI SESWITA ZILDA YULIYANTI YULIANTI RIZKY FAUZIYAH SHOLIHAH SUBARYONO SUBARYONO YUSRO NURI FAWZYA HARI EKO IRIANTO
author_sort	DEWI SESWITA ZILDA
title	A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced
title_short	A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced
title_full	A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced
title_fullStr	A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced
title_full_unstemmed	A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced
title_sort	novel bacillus sp. isolated from rotten seaweed: identification and characterization alginate lyase its produced
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/1c7f09fb109b42a9b89d4433f64fce69
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A novel Bacillus sp. isolated from rotten seaweed: Identification and characterization alginate lyase its produced

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