Detection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-19

Background: The COVID-19 pandemic presents great challenges on transmission prevention, and rapid diagnosis is essential to reduce the disease spread. Various diagnostic methods are available to identify an ongoing infection by nasopharyngeal (NPH) swab sampling. However, the procedure requires hand...

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Autores principales:	Yang De Marinis, Anne-Katrine Pesola, Anna Söderlund Strand, Astrid Norman, Gustav Pernow, Markus Aldén, Runtao Yang, Magnus Rasmussen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	covid-19 sars-cov-2 antigen test saliva Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/1c8084413d17487cb3aacc7b7d39a4e6
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spelling	oai:doaj.org-article:1c8084413d17487cb3aacc7b7d39a4e62021-11-04T15:00:43ZDetection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-192000-868610.1080/20008686.2021.1993535https://doaj.org/article/1c8084413d17487cb3aacc7b7d39a4e62021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/20008686.2021.1993535https://doaj.org/toc/2000-8686Background: The COVID-19 pandemic presents great challenges on transmission prevention, and rapid diagnosis is essential to reduce the disease spread. Various diagnostic methods are available to identify an ongoing infection by nasopharyngeal (NPH) swab sampling. However, the procedure requires handling by health care professionals, and therefore limits the application in household and community settings. Objectives: In this study, we aimed to determine if the detection of SARS-CoV-2 can be performed alternatively on saliva specimens by rapid antigen test. Study Design: Saliva and NPH specimens were collected from 44 patients with confirmed COVID-19. To assess the diagnostic accuracy of point-of-care SARS-CoV-2 rapid antigen test on saliva specimens, we compared the performance of four test products. Results: RT-qPCR was performed and NPH and saliva sampling had similar Ct values, which associated with disease duration. All four antigen tests showed similar trend in detecting SARS-CoV-2 in saliva, but with variation in the ability to detect positive cases. The rapid antigen test with the best performance could detect up to 67% of the positive cases with Ct values lower than 25, and disease duration shorter than 10 days. Conclusion: Our study therefore supports saliva testing as an alternative diagnostic procedure to NPH testing, and that rapid antigen test on saliva provides a potential complement to PCR test to meet increasing screening demand.Yang De MarinisAnne-Katrine PesolaAnna Söderlund StrandAstrid NormanGustav PernowMarkus AldénRuntao YangMagnus RasmussenTaylor & Francis Grouparticlecovid-19sars-cov-2antigen testsalivaInfectious and parasitic diseasesRC109-216ENInfection Ecology & Epidemiology, Vol 11, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	covid-19 sars-cov-2 antigen test saliva Infectious and parasitic diseases RC109-216
spellingShingle	covid-19 sars-cov-2 antigen test saliva Infectious and parasitic diseases RC109-216 Yang De Marinis Anne-Katrine Pesola Anna Söderlund Strand Astrid Norman Gustav Pernow Markus Aldén Runtao Yang Magnus Rasmussen Detection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-19
description	Background: The COVID-19 pandemic presents great challenges on transmission prevention, and rapid diagnosis is essential to reduce the disease spread. Various diagnostic methods are available to identify an ongoing infection by nasopharyngeal (NPH) swab sampling. However, the procedure requires handling by health care professionals, and therefore limits the application in household and community settings. Objectives: In this study, we aimed to determine if the detection of SARS-CoV-2 can be performed alternatively on saliva specimens by rapid antigen test. Study Design: Saliva and NPH specimens were collected from 44 patients with confirmed COVID-19. To assess the diagnostic accuracy of point-of-care SARS-CoV-2 rapid antigen test on saliva specimens, we compared the performance of four test products. Results: RT-qPCR was performed and NPH and saliva sampling had similar Ct values, which associated with disease duration. All four antigen tests showed similar trend in detecting SARS-CoV-2 in saliva, but with variation in the ability to detect positive cases. The rapid antigen test with the best performance could detect up to 67% of the positive cases with Ct values lower than 25, and disease duration shorter than 10 days. Conclusion: Our study therefore supports saliva testing as an alternative diagnostic procedure to NPH testing, and that rapid antigen test on saliva provides a potential complement to PCR test to meet increasing screening demand.
format	article
author	Yang De Marinis Anne-Katrine Pesola Anna Söderlund Strand Astrid Norman Gustav Pernow Markus Aldén Runtao Yang Magnus Rasmussen
author_facet	Yang De Marinis Anne-Katrine Pesola Anna Söderlund Strand Astrid Norman Gustav Pernow Markus Aldén Runtao Yang Magnus Rasmussen
author_sort	Yang De Marinis
title	Detection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-19
title_short	Detection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-19
title_full	Detection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-19
title_fullStr	Detection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-19
title_full_unstemmed	Detection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-19
title_sort	detection of sars-cov-2 by rapid antigen tests on saliva in hospitalized patients with covid-19
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/1c8084413d17487cb3aacc7b7d39a4e6
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Detection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-19

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