Quantification of dendritic cell subsets in human thymus tissues of various ages

Abstract Background Dendritic cells (DCs) in the thymus are involved in central tolerance formation, but they also have other functions in the thymus, such as pathogen recognition. The density changes of human thymic DCs have been hardly investigated. In this study, human thymus samples of various a...

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Autores principales:	Yan Li, Pei Chen, Hao Huang, Huiyu Feng, Hao Ran, Weibin Liu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Dendritic cell Thymus Aging Plasmacytoid dendritic cell Myeloid dendritic cell Immunologic diseases. Allergy RC581-607 Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/1c93f1a6664a448aab1357551badb6ab
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spelling	oai:doaj.org-article:1c93f1a6664a448aab1357551badb6ab2021-11-21T12:06:32ZQuantification of dendritic cell subsets in human thymus tissues of various ages10.1186/s12979-021-00255-81742-4933https://doaj.org/article/1c93f1a6664a448aab1357551badb6ab2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12979-021-00255-8https://doaj.org/toc/1742-4933Abstract Background Dendritic cells (DCs) in the thymus are involved in central tolerance formation, but they also have other functions in the thymus, such as pathogen recognition. The density changes of human thymic DCs have been hardly investigated. In this study, human thymus samples of various ages were collected for tissue sectioning and staining. The thymic cortex and medulla area as well as the densities of various subsets of thymic DCs were calculated. Results All common DC subsets were found in the human thymus of various ages. Most DCs had accumulated in the human thymic epithelial space, especially the medulla. We also found that the human thymic cortex had atrophied relatively faster than the medulla, which led to a gradual increase of the area ratio of the medulla to cortex with the increase of age. The densities of DC subsets in the human thymus showed various changes with increasing age, which contributed to the composition changes of DC subsets. The density of plasmacytoid DCs (pDCs) in the human thymus had increased gradually with aging, which suggested that pDCs plays another essential role in the thymus in addition to central tolerance. Conclusions Inconsistent with the shrinking of the epithelial space in the thymus, the densities of DC subsets in the epithelial space of the thymus are maintained at a constant level with aging to preserve highly efficient autoreactive thymocyte screening. An increasing density of the thymic pDCs with aging implies an extra function of DCs in the thymus beyond central tolerance.Yan LiPei ChenHao HuangHuiyu FengHao RanWeibin LiuBMCarticleDendritic cellThymusAgingPlasmacytoid dendritic cellMyeloid dendritic cellImmunologic diseases. AllergyRC581-607GeriatricsRC952-954.6ENImmunity & Ageing, Vol 18, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dendritic cell Thymus Aging Plasmacytoid dendritic cell Myeloid dendritic cell Immunologic diseases. Allergy RC581-607 Geriatrics RC952-954.6
spellingShingle	Dendritic cell Thymus Aging Plasmacytoid dendritic cell Myeloid dendritic cell Immunologic diseases. Allergy RC581-607 Geriatrics RC952-954.6 Yan Li Pei Chen Hao Huang Huiyu Feng Hao Ran Weibin Liu Quantification of dendritic cell subsets in human thymus tissues of various ages
description	Abstract Background Dendritic cells (DCs) in the thymus are involved in central tolerance formation, but they also have other functions in the thymus, such as pathogen recognition. The density changes of human thymic DCs have been hardly investigated. In this study, human thymus samples of various ages were collected for tissue sectioning and staining. The thymic cortex and medulla area as well as the densities of various subsets of thymic DCs were calculated. Results All common DC subsets were found in the human thymus of various ages. Most DCs had accumulated in the human thymic epithelial space, especially the medulla. We also found that the human thymic cortex had atrophied relatively faster than the medulla, which led to a gradual increase of the area ratio of the medulla to cortex with the increase of age. The densities of DC subsets in the human thymus showed various changes with increasing age, which contributed to the composition changes of DC subsets. The density of plasmacytoid DCs (pDCs) in the human thymus had increased gradually with aging, which suggested that pDCs plays another essential role in the thymus in addition to central tolerance. Conclusions Inconsistent with the shrinking of the epithelial space in the thymus, the densities of DC subsets in the epithelial space of the thymus are maintained at a constant level with aging to preserve highly efficient autoreactive thymocyte screening. An increasing density of the thymic pDCs with aging implies an extra function of DCs in the thymus beyond central tolerance.
format	article
author	Yan Li Pei Chen Hao Huang Huiyu Feng Hao Ran Weibin Liu
author_facet	Yan Li Pei Chen Hao Huang Huiyu Feng Hao Ran Weibin Liu
author_sort	Yan Li
title	Quantification of dendritic cell subsets in human thymus tissues of various ages
title_short	Quantification of dendritic cell subsets in human thymus tissues of various ages
title_full	Quantification of dendritic cell subsets in human thymus tissues of various ages
title_fullStr	Quantification of dendritic cell subsets in human thymus tissues of various ages
title_full_unstemmed	Quantification of dendritic cell subsets in human thymus tissues of various ages
title_sort	quantification of dendritic cell subsets in human thymus tissues of various ages
publisher	BMC
publishDate	2021
url	https://doaj.org/article/1c93f1a6664a448aab1357551badb6ab
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Quantification of dendritic cell subsets in human thymus tissues of various ages

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