Using Knock-Out Mutants to Investigate the Adhesion of Staphylococcus aureus to Abiotic Surfaces

The adhesion of Staphylococcus aureus to abiotic surfaces is crucial for establishing device-related infections. With a high number of single-cell force spectroscopy measurements with genetically modified S. aureus cells, this study provides insights into the ad...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Christian Spengler, Friederike Nolle, Nicolas Thewes, Ben Wieland, Philipp Jung, Markus Bischoff, Karin Jacobs
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bacterial adhesion single-cell force spectroscopy <i>Staphylococcus aureus</i> <i>Staphylococcus aureus</i> knock-out mutants surface charge hydrophobicity Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1c9679d3c5334931a5a9ea80b3269be7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1c9679d3c5334931a5a9ea80b3269be7
record_format	dspace
spelling	oai:doaj.org-article:1c9679d3c5334931a5a9ea80b3269be72021-11-11T17:22:15ZUsing Knock-Out Mutants to Investigate the Adhesion of <i>Staphylococcus aureus</i> to Abiotic Surfaces10.3390/ijms2221119521422-00671661-6596https://doaj.org/article/1c9679d3c5334931a5a9ea80b3269be72021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11952https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The adhesion of <i>Staphylococcus aureus</i> to abiotic surfaces is crucial for establishing device-related infections. With a high number of single-cell force spectroscopy measurements with genetically modified <i>S. aureus</i> cells, this study provides insights into the adhesion process of the pathogen to abiotic surfaces of different wettability. Our results show that <i>S. aureus</i> utilizes different cell wall molecules and interaction mechanisms when binding to hydrophobic and hydrophilic surfaces. We found that covalently bound cell wall proteins strongly interact with hydrophobic substrates, while their contribution to the overall adhesion force is smaller on hydrophilic substrates. Teichoic acids promote adhesion to hydrophobic surfaces as well as to hydrophilic surfaces. This, however, is to a lesser extent. An interplay of electrostatic effects of charges and protein composition on bacterial surfaces is predominant on hydrophilic surfaces, while it is overshadowed on hydrophobic surfaces by the influence of the high number of binding proteins. Our results can help to design new models of bacterial adhesion and may be used to interpret the adhesion of other microorganisms with similar surface properties.Christian SpenglerFriederike NolleNicolas ThewesBen WielandPhilipp JungMarkus BischoffKarin JacobsMDPI AGarticlebacterial adhesionsingle-cell force spectroscopy<i>Staphylococcus aureus</i><i>Staphylococcus aureus</i> knock-out mutantssurface chargehydrophobicityBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11952, p 11952 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial adhesion single-cell force spectroscopy <i>Staphylococcus aureus</i> <i>Staphylococcus aureus</i> knock-out mutants surface charge hydrophobicity Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	bacterial adhesion single-cell force spectroscopy <i>Staphylococcus aureus</i> <i>Staphylococcus aureus</i> knock-out mutants surface charge hydrophobicity Biology (General) QH301-705.5 Chemistry QD1-999 Christian Spengler Friederike Nolle Nicolas Thewes Ben Wieland Philipp Jung Markus Bischoff Karin Jacobs Using Knock-Out Mutants to Investigate the Adhesion of <i>Staphylococcus aureus</i> to Abiotic Surfaces
description	The adhesion of <i>Staphylococcus aureus</i> to abiotic surfaces is crucial for establishing device-related infections. With a high number of single-cell force spectroscopy measurements with genetically modified <i>S. aureus</i> cells, this study provides insights into the adhesion process of the pathogen to abiotic surfaces of different wettability. Our results show that <i>S. aureus</i> utilizes different cell wall molecules and interaction mechanisms when binding to hydrophobic and hydrophilic surfaces. We found that covalently bound cell wall proteins strongly interact with hydrophobic substrates, while their contribution to the overall adhesion force is smaller on hydrophilic substrates. Teichoic acids promote adhesion to hydrophobic surfaces as well as to hydrophilic surfaces. This, however, is to a lesser extent. An interplay of electrostatic effects of charges and protein composition on bacterial surfaces is predominant on hydrophilic surfaces, while it is overshadowed on hydrophobic surfaces by the influence of the high number of binding proteins. Our results can help to design new models of bacterial adhesion and may be used to interpret the adhesion of other microorganisms with similar surface properties.
format	article
author	Christian Spengler Friederike Nolle Nicolas Thewes Ben Wieland Philipp Jung Markus Bischoff Karin Jacobs
author_facet	Christian Spengler Friederike Nolle Nicolas Thewes Ben Wieland Philipp Jung Markus Bischoff Karin Jacobs
author_sort	Christian Spengler
title	Using Knock-Out Mutants to Investigate the Adhesion of <i>Staphylococcus aureus</i> to Abiotic Surfaces
title_short	Using Knock-Out Mutants to Investigate the Adhesion of <i>Staphylococcus aureus</i> to Abiotic Surfaces
title_full	Using Knock-Out Mutants to Investigate the Adhesion of <i>Staphylococcus aureus</i> to Abiotic Surfaces
title_fullStr	Using Knock-Out Mutants to Investigate the Adhesion of <i>Staphylococcus aureus</i> to Abiotic Surfaces
title_full_unstemmed	Using Knock-Out Mutants to Investigate the Adhesion of <i>Staphylococcus aureus</i> to Abiotic Surfaces
title_sort	using knock-out mutants to investigate the adhesion of <i>staphylococcus aureus</i> to abiotic surfaces
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1c9679d3c5334931a5a9ea80b3269be7
work_keys_str_mv	AT christianspengler usingknockoutmutantstoinvestigatetheadhesionofistaphylococcusaureusitoabioticsurfaces AT friederikenolle usingknockoutmutantstoinvestigatetheadhesionofistaphylococcusaureusitoabioticsurfaces AT nicolasthewes usingknockoutmutantstoinvestigatetheadhesionofistaphylococcusaureusitoabioticsurfaces AT benwieland usingknockoutmutantstoinvestigatetheadhesionofistaphylococcusaureusitoabioticsurfaces AT philippjung usingknockoutmutantstoinvestigatetheadhesionofistaphylococcusaureusitoabioticsurfaces AT markusbischoff usingknockoutmutantstoinvestigatetheadhesionofistaphylococcusaureusitoabioticsurfaces AT karinjacobs usingknockoutmutantstoinvestigatetheadhesionofistaphylococcusaureusitoabioticsurfaces
_version_	1718432103959363584

Using Knock-Out Mutants to Investigate the Adhesion of <i>Staphylococcus aureus</i> to Abiotic Surfaces

Ejemplares similares