MIL-101-NH<sub>2</sub>-mediated nucleic acid delivery and gene silencing
Nucleic acids, including DNA and RNA, can participate in the regulation of gene expression in all organisms. However, those nucleic acid molecules are easily to be degraded. If they are directly administered orally or intravenously, their bioavailability is extremely low, so suitable carriers are re...
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Academic Journals Center of Shanghai Normal University
2021
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oai:doaj.org-article:1c9f299383cc462ea48abae017c970ac2021-11-12T03:34:00ZMIL-101-NH<sub>2</sub>-mediated nucleic acid delivery and gene silencing1000-513710.3969/J.ISSN.1000-5137.2021.05.006https://doaj.org/article/1c9f299383cc462ea48abae017c970ac2021-10-01T00:00:00Zhttp://qktg.shnu.edu.cn/zrb/shsfqkszrb/ch/reader/view_abstract.aspx?file_no=20210506https://doaj.org/toc/1000-5137Nucleic acids, including DNA and RNA, can participate in the regulation of gene expression in all organisms. However, those nucleic acid molecules are easily to be degraded. If they are directly administered orally or intravenously, their bioavailability is extremely low, so suitable carriers are required for intracellular delivery of nucleic acids. In this work, the iron-based metal-organic framework(MOF) MIL-101-NH<sub>2</sub> with better biocompatibility is selected to carry single-stranded DNA and siRNA, and its intracellular behavior was explored. MIL-101-NH<sub>2</sub>can effectively deliver single-stranded DNA and siRNA into cells, and siRNA can escape from the lysosomes in cells and function as gene silencing reagent. Our results indicate that MIL-101-NH<sub>2</sub>-based nanocomposite is a promising strategy for nucleic acid delivery and gene regulation.AI YichunYUAN XuejiaoYU YichengLYU MinCHEN NanAcademic Journals Center of Shanghai Normal Universityarticlemetal-organic framework(mof)nucleic acid moleculesirnagene silencingScience (General)Q1-390ENZHJournal of Shanghai Normal University (Natural Sciences), Vol 50, Iss 5, Pp 559-566 (2021) |
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DOAJ |
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DOAJ |
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EN ZH |
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metal-organic framework(mof) nucleic acid molecule sirna gene silencing Science (General) Q1-390 |
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metal-organic framework(mof) nucleic acid molecule sirna gene silencing Science (General) Q1-390 AI Yichun YUAN Xuejiao YU Yicheng LYU Min CHEN Nan MIL-101-NH<sub>2</sub>-mediated nucleic acid delivery and gene silencing |
| description |
Nucleic acids, including DNA and RNA, can participate in the regulation of gene expression in all organisms. However, those nucleic acid molecules are easily to be degraded. If they are directly administered orally or intravenously, their bioavailability is extremely low, so suitable carriers are required for intracellular delivery of nucleic acids. In this work, the iron-based metal-organic framework(MOF) MIL-101-NH<sub>2</sub> with better biocompatibility is selected to carry single-stranded DNA and siRNA, and its intracellular behavior was explored. MIL-101-NH<sub>2</sub>can effectively deliver single-stranded DNA and siRNA into cells, and siRNA can escape from the lysosomes in cells and function as gene silencing reagent. Our results indicate that MIL-101-NH<sub>2</sub>-based nanocomposite is a promising strategy for nucleic acid delivery and gene regulation. |
| format |
article |
| author |
AI Yichun YUAN Xuejiao YU Yicheng LYU Min CHEN Nan |
| author_facet |
AI Yichun YUAN Xuejiao YU Yicheng LYU Min CHEN Nan |
| author_sort |
AI Yichun |
| title |
MIL-101-NH<sub>2</sub>-mediated nucleic acid delivery and gene silencing |
| title_short |
MIL-101-NH<sub>2</sub>-mediated nucleic acid delivery and gene silencing |
| title_full |
MIL-101-NH<sub>2</sub>-mediated nucleic acid delivery and gene silencing |
| title_fullStr |
MIL-101-NH<sub>2</sub>-mediated nucleic acid delivery and gene silencing |
| title_full_unstemmed |
MIL-101-NH<sub>2</sub>-mediated nucleic acid delivery and gene silencing |
| title_sort |
mil-101-nh<sub>2</sub>-mediated nucleic acid delivery and gene silencing |
| publisher |
Academic Journals Center of Shanghai Normal University |
| publishDate |
2021 |
| url |
https://doaj.org/article/1c9f299383cc462ea48abae017c970ac |
| work_keys_str_mv |
AT aiyichun mil101nhsub2submediatednucleicaciddeliveryandgenesilencing AT yuanxuejiao mil101nhsub2submediatednucleicaciddeliveryandgenesilencing AT yuyicheng mil101nhsub2submediatednucleicaciddeliveryandgenesilencing AT lyumin mil101nhsub2submediatednucleicaciddeliveryandgenesilencing AT chennan mil101nhsub2submediatednucleicaciddeliveryandgenesilencing |
| _version_ |
1718431275980685312 |