Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis

Abstract Because of ubiquity of thioesters, thioesterases play a critical role in metabolism, membrane biosynthesis, signal transduction, and gene regulation. In many bacteria, YbgC is such an enzyme, whose coding gene mostly resides in the tol-pal cluster. Although all other proteins encoded in the...

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Autores principales:	Tong Gao, Qiu Meng, Haichun Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ca78d4ab2e1468eb85c4f7233afe572
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spelling	oai:doaj.org-article:1ca78d4ab2e1468eb85c4f7233afe5722021-12-02T15:05:57ZThioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis10.1038/s41598-017-04285-52045-2322https://doaj.org/article/1ca78d4ab2e1468eb85c4f7233afe5722017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04285-5https://doaj.org/toc/2045-2322Abstract Because of ubiquity of thioesters, thioesterases play a critical role in metabolism, membrane biosynthesis, signal transduction, and gene regulation. In many bacteria, YbgC is such an enzyme, whose coding gene mostly resides in the tol-pal cluster. Although all other proteins encoded in the tol-pal cluster are clearly involved in maintaining cell envelope integrity and cell division, little is known about the physiological role of YbgC. In this study, we identify in Shewanella oneidensis, a γ-proteobacterium used as a research model for environmental microbes, YbgC as a motility regulator. The loss of YbgC results in enhanced motility, which is likely due to the increased rotation rate of the flagellum. The regulatory function of YbgC requires its thioesterase activity but could not be replaced by YbgC homologues of other bacteria. We further show that the regulation of YbgC is mediated by the second message c-di-GMP.Tong GaoQiu MengHaichun GaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tong Gao Qiu Meng Haichun Gao Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis
description	Abstract Because of ubiquity of thioesters, thioesterases play a critical role in metabolism, membrane biosynthesis, signal transduction, and gene regulation. In many bacteria, YbgC is such an enzyme, whose coding gene mostly resides in the tol-pal cluster. Although all other proteins encoded in the tol-pal cluster are clearly involved in maintaining cell envelope integrity and cell division, little is known about the physiological role of YbgC. In this study, we identify in Shewanella oneidensis, a γ-proteobacterium used as a research model for environmental microbes, YbgC as a motility regulator. The loss of YbgC results in enhanced motility, which is likely due to the increased rotation rate of the flagellum. The regulatory function of YbgC requires its thioesterase activity but could not be replaced by YbgC homologues of other bacteria. We further show that the regulation of YbgC is mediated by the second message c-di-GMP.
format	article
author	Tong Gao Qiu Meng Haichun Gao
author_facet	Tong Gao Qiu Meng Haichun Gao
author_sort	Tong Gao
title	Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis
title_short	Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis
title_full	Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis
title_fullStr	Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis
title_full_unstemmed	Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis
title_sort	thioesterase ybgc affects motility by modulating c-di-gmp levels in shewanella oneidensis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1ca78d4ab2e1468eb85c4f7233afe572
work_keys_str_mv	AT tonggao thioesteraseybgcaffectsmotilitybymodulatingcdigmplevelsinshewanellaoneidensis AT qiumeng thioesteraseybgcaffectsmotilitybymodulatingcdigmplevelsinshewanellaoneidensis AT haichungao thioesteraseybgcaffectsmotilitybymodulatingcdigmplevelsinshewanellaoneidensis
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Thioesterase YbgC affects motility by modulating c-di-GMP levels in Shewanella oneidensis

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