Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi

Abstract Borrelia miyamotoi, a member of the tick-borne relapsing fever spirochetes, shows a serum-resistant phenotype in vitro. This ability of B. miyamotoi may contribute to bacterial evasion of the host innate immune system. To investigate the molecular mechanism of serum-resistance, we construct...

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Autores principales:	Kozue Sato, Yumi Kumagai, Tsuyoshi Sekizuka, Makoto Kuroda, Tetsuya Hayashi, Ai Takano, Gaowa, Kyle R. Taylor, Makoto Ohnishi, Hiroki Kawabata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ca93b4dd78d42f4a47f6c2bc832432d
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spelling	oai:doaj.org-article:1ca93b4dd78d42f4a47f6c2bc832432d2021-12-02T13:30:50ZVitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi10.1038/s41598-021-85069-w2045-2322https://doaj.org/article/1ca93b4dd78d42f4a47f6c2bc832432d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85069-whttps://doaj.org/toc/2045-2322Abstract Borrelia miyamotoi, a member of the tick-borne relapsing fever spirochetes, shows a serum-resistant phenotype in vitro. This ability of B. miyamotoi may contribute to bacterial evasion of the host innate immune system. To investigate the molecular mechanism of serum-resistance, we constructed a membrane protein-encoding gene library of B. miyamotoi using Borrelia garinii strain HT59G, which shows a transformable and serum-susceptible phenotype. By screening the library, we found that bom1093 and bom1515 of B. miyamotoi provided a serum-resistant phenotype to the recipient B. garinii. These B. miyamotoi genes are predicted to encode P35-like antigen genes and are conserved among relapsing fever borreliae. Functional analysis revealed that BOM1093 bound to serum vitronectin and that the C-terminal region of BOM1093 was involved in the vitronectin-binding property. Importantly, the B. garinii transformant was not serum-resistant when the C terminus-truncated BOM1093 was expressed. We also observed that the depletion of vitronectin from human serum enhances the bactericidal activity of BOM1093 expressing B. garinii, and the survival rate of BOM1093 expressing B. garinii in vitronectin-depleted serum is enhanced by the addition of purified vitronectin. Our data suggests that B. miyamotoi utilize BOM1093-mediated binding to vitronectin as a mechanism of serum resistance.Kozue SatoYumi KumagaiTsuyoshi SekizukaMakoto KurodaTetsuya HayashiAi TakanoGaowaKyle R. TaylorMakoto OhnishiHiroki KawabataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kozue Sato Yumi Kumagai Tsuyoshi Sekizuka Makoto Kuroda Tetsuya Hayashi Ai Takano Gaowa Kyle R. Taylor Makoto Ohnishi Hiroki Kawabata Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi
description	Abstract Borrelia miyamotoi, a member of the tick-borne relapsing fever spirochetes, shows a serum-resistant phenotype in vitro. This ability of B. miyamotoi may contribute to bacterial evasion of the host innate immune system. To investigate the molecular mechanism of serum-resistance, we constructed a membrane protein-encoding gene library of B. miyamotoi using Borrelia garinii strain HT59G, which shows a transformable and serum-susceptible phenotype. By screening the library, we found that bom1093 and bom1515 of B. miyamotoi provided a serum-resistant phenotype to the recipient B. garinii. These B. miyamotoi genes are predicted to encode P35-like antigen genes and are conserved among relapsing fever borreliae. Functional analysis revealed that BOM1093 bound to serum vitronectin and that the C-terminal region of BOM1093 was involved in the vitronectin-binding property. Importantly, the B. garinii transformant was not serum-resistant when the C terminus-truncated BOM1093 was expressed. We also observed that the depletion of vitronectin from human serum enhances the bactericidal activity of BOM1093 expressing B. garinii, and the survival rate of BOM1093 expressing B. garinii in vitronectin-depleted serum is enhanced by the addition of purified vitronectin. Our data suggests that B. miyamotoi utilize BOM1093-mediated binding to vitronectin as a mechanism of serum resistance.
format	article
author	Kozue Sato Yumi Kumagai Tsuyoshi Sekizuka Makoto Kuroda Tetsuya Hayashi Ai Takano Gaowa Kyle R. Taylor Makoto Ohnishi Hiroki Kawabata
author_facet	Kozue Sato Yumi Kumagai Tsuyoshi Sekizuka Makoto Kuroda Tetsuya Hayashi Ai Takano Gaowa Kyle R. Taylor Makoto Ohnishi Hiroki Kawabata
author_sort	Kozue Sato
title	Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi
title_short	Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi
title_full	Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi
title_fullStr	Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi
title_full_unstemmed	Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi
title_sort	vitronectin binding protein, bom1093, confers serum resistance on borrelia miyamotoi
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1ca93b4dd78d42f4a47f6c2bc832432d
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Vitronectin binding protein, BOM1093, confers serum resistance on Borrelia miyamotoi

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