Assessment of Serum Vitamin B12 and Folate Levels and Macrocytosis in Patients with Type 2 Diabetes Mellitus on Metformin Attending Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia: A Cross-Sectional Study
Wondwossen Wale Tesega, 1 Solomon Genet, 2 Gnanasekeran Netasan, 2 Getahun Tarekegn, 2 Fitsum Girma, 3 Dinkinesh Chalchisa, 3 Yohannes Belay, 3 Zeleke Geto, 4 Tadesse Asmamaw Dejenie 5 1Department of Biomedical Sciences, School of Medicine, College of Health Sciences, Arbaminch University, Arba Min...
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Dove Medical Press
2021
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oai:doaj.org-article:1cae46ac27f84560a01d4a7ef491e59a2021-12-02T15:42:48ZAssessment of Serum Vitamin B12 and Folate Levels and Macrocytosis in Patients with Type 2 Diabetes Mellitus on Metformin Attending Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia: A Cross-Sectional Study1178-7007https://doaj.org/article/1cae46ac27f84560a01d4a7ef491e59a2021-05-01T00:00:00Zhttps://www.dovepress.com/assessment-of-serum-vitamin-b12-and-folate-levels-and-macrocytosis-in--peer-reviewed-fulltext-article-DMSOhttps://doaj.org/toc/1178-7007Wondwossen Wale Tesega, 1 Solomon Genet, 2 Gnanasekeran Netasan, 2 Getahun Tarekegn, 2 Fitsum Girma, 3 Dinkinesh Chalchisa, 3 Yohannes Belay, 3 Zeleke Geto, 4 Tadesse Asmamaw Dejenie 5 1Department of Biomedical Sciences, School of Medicine, College of Health Sciences, Arbaminch University, Arba Minch, Ethiopia; 2Department of Medical Biochemistry, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 3National Reference Laboratory for Immunohematology, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 4Department of Biomedical Science, College of Medicine and Health Science, Wollo University, Dessie, Ethiopia; 5Department of Biochemistry, School of Medicine, College of Medicine and Health Science, University of Gondar, Gondar, EthiopiaCorrespondence: Tadesse Asmamaw Dejenie Tel +251 90-904-5760Email as24tadesse@gmail.comBackground: Metformin is the first-line drug in the treatment of type 2 diabetes mellitus. Monitoring vitamin B 12 deficiency associated with long-term and high-dose therapy is not a common practice in many clinical settings in Ethiopia.Objective: The study aimed to measure levels of serum vitamin B 12 and folate and to assess the macrocytic status of type 2 diabetes mellitus patients on metformin.Methods: A cross-sectional study was conducted on 80 type 2 diabetes mellitus patients who had been on metformin for 5 months or more at the diabetic clinic of Tikur Anbessa Specialized Teaching Hospital. Serum vitamin B 12 and folate levels were quantified by chemiluminescent immunoassays. Mean corpuscular volume was determined by complete blood count. Differences in vitamin B 12 and folate levels and mean corpuscular volume between different groups were assessed using Kruskal–Wallis H and Mann–Whitney U tests.Results: Vitamin B 12 and folate deficiency were documented in 5% and 23.8% of participants, respectively, and 6.2% of patients were macrocytic. Levels of vitamin B 12 and folate in patients who had been on metformin > 1,500 mg/day ≥ 4 years were significantly lower those who had been on metformin 1,000– 1,500 mg/day and < 1,000 mg/day < 4 years, respectively.Conclusion: Low serum vitamin B 12 and folate levels and macrocytosis were found to be associated with prolonged metformin treatment.Keywords: macrocytosis, metformin, type 2 diabetes mellitus, vitamin B 12, folateWale Tesega WGenet SNatesan GTarekegn GGirma FChalchisa DBelay YGeto ZAsmamaw Dejenie TDove Medical Pressarticlemacrocytosismetformintype 2 diabetes mellitusvitamin b12folateSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 2011-2018 (2021) |
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EN |
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macrocytosis metformin type 2 diabetes mellitus vitamin b12 folate Specialties of internal medicine RC581-951 |
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macrocytosis metformin type 2 diabetes mellitus vitamin b12 folate Specialties of internal medicine RC581-951 Wale Tesega W Genet S Natesan G Tarekegn G Girma F Chalchisa D Belay Y Geto Z Asmamaw Dejenie T Assessment of Serum Vitamin B12 and Folate Levels and Macrocytosis in Patients with Type 2 Diabetes Mellitus on Metformin Attending Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia: A Cross-Sectional Study |
| description |
Wondwossen Wale Tesega, 1 Solomon Genet, 2 Gnanasekeran Netasan, 2 Getahun Tarekegn, 2 Fitsum Girma, 3 Dinkinesh Chalchisa, 3 Yohannes Belay, 3 Zeleke Geto, 4 Tadesse Asmamaw Dejenie 5 1Department of Biomedical Sciences, School of Medicine, College of Health Sciences, Arbaminch University, Arba Minch, Ethiopia; 2Department of Medical Biochemistry, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 3National Reference Laboratory for Immunohematology, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 4Department of Biomedical Science, College of Medicine and Health Science, Wollo University, Dessie, Ethiopia; 5Department of Biochemistry, School of Medicine, College of Medicine and Health Science, University of Gondar, Gondar, EthiopiaCorrespondence: Tadesse Asmamaw Dejenie Tel +251 90-904-5760Email as24tadesse@gmail.comBackground: Metformin is the first-line drug in the treatment of type 2 diabetes mellitus. Monitoring vitamin B 12 deficiency associated with long-term and high-dose therapy is not a common practice in many clinical settings in Ethiopia.Objective: The study aimed to measure levels of serum vitamin B 12 and folate and to assess the macrocytic status of type 2 diabetes mellitus patients on metformin.Methods: A cross-sectional study was conducted on 80 type 2 diabetes mellitus patients who had been on metformin for 5 months or more at the diabetic clinic of Tikur Anbessa Specialized Teaching Hospital. Serum vitamin B 12 and folate levels were quantified by chemiluminescent immunoassays. Mean corpuscular volume was determined by complete blood count. Differences in vitamin B 12 and folate levels and mean corpuscular volume between different groups were assessed using Kruskal–Wallis H and Mann–Whitney U tests.Results: Vitamin B 12 and folate deficiency were documented in 5% and 23.8% of participants, respectively, and 6.2% of patients were macrocytic. Levels of vitamin B 12 and folate in patients who had been on metformin > 1,500 mg/day ≥ 4 years were significantly lower those who had been on metformin 1,000– 1,500 mg/day and < 1,000 mg/day < 4 years, respectively.Conclusion: Low serum vitamin B 12 and folate levels and macrocytosis were found to be associated with prolonged metformin treatment.Keywords: macrocytosis, metformin, type 2 diabetes mellitus, vitamin B 12, folate |
| format |
article |
| author |
Wale Tesega W Genet S Natesan G Tarekegn G Girma F Chalchisa D Belay Y Geto Z Asmamaw Dejenie T |
| author_facet |
Wale Tesega W Genet S Natesan G Tarekegn G Girma F Chalchisa D Belay Y Geto Z Asmamaw Dejenie T |
| author_sort |
Wale Tesega W |
| title |
Assessment of Serum Vitamin B12 and Folate Levels and Macrocytosis in Patients with Type 2 Diabetes Mellitus on Metformin Attending Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia: A Cross-Sectional Study |
| title_short |
Assessment of Serum Vitamin B12 and Folate Levels and Macrocytosis in Patients with Type 2 Diabetes Mellitus on Metformin Attending Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia: A Cross-Sectional Study |
| title_full |
Assessment of Serum Vitamin B12 and Folate Levels and Macrocytosis in Patients with Type 2 Diabetes Mellitus on Metformin Attending Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia: A Cross-Sectional Study |
| title_fullStr |
Assessment of Serum Vitamin B12 and Folate Levels and Macrocytosis in Patients with Type 2 Diabetes Mellitus on Metformin Attending Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia: A Cross-Sectional Study |
| title_full_unstemmed |
Assessment of Serum Vitamin B12 and Folate Levels and Macrocytosis in Patients with Type 2 Diabetes Mellitus on Metformin Attending Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia: A Cross-Sectional Study |
| title_sort |
assessment of serum vitamin b12 and folate levels and macrocytosis in patients with type 2 diabetes mellitus on metformin attending tikur anbessa specialized hospital, addis ababa, ethiopia: a cross-sectional study |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/1cae46ac27f84560a01d4a7ef491e59a |
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