Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts

Abstract Estrus expression by gilts and sows is hereditable and important for heat detection. To better understand the molecular biological mechanisms of estrus expression in gilts, the mRNA expression profiles of follicular tissue from Large White gilts in diestrus (LD, n = 3) and estrus (LE, n = 3...

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Autores principales:	Qingpo Chu, Bo Zhou, Feilong Xu, Ruonan Chen, Chunyan Shen, Tingting Liang, Yuan Li, Allan P. Schinckel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1cbcaf56465e48cea233245e6d7aa58f
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spelling	oai:doaj.org-article:1cbcaf56465e48cea233245e6d7aa58f2021-12-02T12:32:08ZGenome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts10.1038/s41598-017-04336-x2045-2322https://doaj.org/article/1cbcaf56465e48cea233245e6d7aa58f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04336-xhttps://doaj.org/toc/2045-2322Abstract Estrus expression by gilts and sows is hereditable and important for heat detection. To better understand the molecular biological mechanisms of estrus expression in gilts, the mRNA expression profiles of follicular tissue from Large White gilts in diestrus (LD, n = 3) and estrus (LE, n = 3), and Chinese indigenous Mi gilts in diestrus (MD, n = 2) and estrus (ME, n = 3) were investigated using RNA sequencing. We detected 122,804-335,295 SNPs, 6,140-14,947 InDel and 12 types of AS events (39.57% TSS, 34.90% TTS) in 11 samples. A total of 2,838 differentially expressed genes (DEGs) were found in LD vs MD, LE vs ME, LE vs LD, or ME vs MD comparisons. Two DEGs (ACP5 and PIGS) were observed in all comparisons. Two new genes (ENSSSCG00000028235 and ENSSSCG00000021903) were exclusively expressed in Mi and Large White gilts, respectively. Bioinformatics analyses indicate that these DEGs are involved in single-organism process, catalytic activity, cell adhesion and enriched in ECM-receptor interaction, olfactory transduction, ovarian steroidogenesis, steroid biosynthesis and CAMs signaling pathways. These results of RNA-Seq have provided important information for screening the key functional genes or molecular markers of estrus expression in gilts.Qingpo ChuBo ZhouFeilong XuRuonan ChenChunyan ShenTingting LiangYuan LiAllan P. SchinckelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qingpo Chu Bo Zhou Feilong Xu Ruonan Chen Chunyan Shen Tingting Liang Yuan Li Allan P. Schinckel Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
description	Abstract Estrus expression by gilts and sows is hereditable and important for heat detection. To better understand the molecular biological mechanisms of estrus expression in gilts, the mRNA expression profiles of follicular tissue from Large White gilts in diestrus (LD, n = 3) and estrus (LE, n = 3), and Chinese indigenous Mi gilts in diestrus (MD, n = 2) and estrus (ME, n = 3) were investigated using RNA sequencing. We detected 122,804-335,295 SNPs, 6,140-14,947 InDel and 12 types of AS events (39.57% TSS, 34.90% TTS) in 11 samples. A total of 2,838 differentially expressed genes (DEGs) were found in LD vs MD, LE vs ME, LE vs LD, or ME vs MD comparisons. Two DEGs (ACP5 and PIGS) were observed in all comparisons. Two new genes (ENSSSCG00000028235 and ENSSSCG00000021903) were exclusively expressed in Mi and Large White gilts, respectively. Bioinformatics analyses indicate that these DEGs are involved in single-organism process, catalytic activity, cell adhesion and enriched in ECM-receptor interaction, olfactory transduction, ovarian steroidogenesis, steroid biosynthesis and CAMs signaling pathways. These results of RNA-Seq have provided important information for screening the key functional genes or molecular markers of estrus expression in gilts.
format	article
author	Qingpo Chu Bo Zhou Feilong Xu Ruonan Chen Chunyan Shen Tingting Liang Yuan Li Allan P. Schinckel
author_facet	Qingpo Chu Bo Zhou Feilong Xu Ruonan Chen Chunyan Shen Tingting Liang Yuan Li Allan P. Schinckel
author_sort	Qingpo Chu
title	Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
title_short	Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
title_full	Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
title_fullStr	Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
title_full_unstemmed	Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
title_sort	genome-wide differential mrna expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1cbcaf56465e48cea233245e6d7aa58f
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Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts

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