C. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways

Abstract The nematode C. elegans is a leading model to investigate the mechanisms of stress-induced behavioral changes coupled with biochemical mechanisms. Our group has previously characterized C. elegans behavior using a microfluidic-based electrotaxis device, and showed that worms display directi...

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Autores principales:	Shane K. B. Taylor, Muhammad H. Minhas, Justin Tong, P. Ravi Selvaganapathy, Ram K. Mishra, Bhagwati P. Gupta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1cbde93eba784304bb79dd9f9b099913
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spelling	oai:doaj.org-article:1cbde93eba784304bb79dd9f9b0999132021-12-02T14:06:50ZC. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways10.1038/s41598-021-82466-z2045-2322https://doaj.org/article/1cbde93eba784304bb79dd9f9b0999132021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82466-zhttps://doaj.org/toc/2045-2322Abstract The nematode C. elegans is a leading model to investigate the mechanisms of stress-induced behavioral changes coupled with biochemical mechanisms. Our group has previously characterized C. elegans behavior using a microfluidic-based electrotaxis device, and showed that worms display directional motion in the presence of a mild electric field. In this study, we describe the effects of various forms of genetic and environmental stress on the electrotactic movement of animals. Using exposure to chemicals, such as paraquat and tunicamycin, as well as mitochondrial and endoplasmic reticulum (ER) unfolded protein response (UPR) mutants, we demonstrate that chronic stress causes abnormal movement. Additionally, we report that pqe-1 (human RNA exonuclease 1 homolog) is necessary for the maintenance of multiple stress response signaling and electrotaxis behavior of animals. Further, exposure of C. elegans to several environmental stress-inducing conditions revealed that while chronic heat and dietary restriction caused electrotaxis speed deficits due to prolonged stress, daily exercise had a beneficial effect on the animals, likely due to improved muscle health and transient activation of UPR. Overall, these data demonstrate that the electrotaxis behavior of worms is susceptible to cytosolic, mitochondrial, and ER stress, and that multiple stress response pathways contribute to its preservation in the face of stressful stimuli.Shane K. B. TaylorMuhammad H. MinhasJustin TongP. Ravi SelvaganapathyRam K. MishraBhagwati P. GuptaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shane K. B. Taylor Muhammad H. Minhas Justin Tong P. Ravi Selvaganapathy Ram K. Mishra Bhagwati P. Gupta C. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways
description	Abstract The nematode C. elegans is a leading model to investigate the mechanisms of stress-induced behavioral changes coupled with biochemical mechanisms. Our group has previously characterized C. elegans behavior using a microfluidic-based electrotaxis device, and showed that worms display directional motion in the presence of a mild electric field. In this study, we describe the effects of various forms of genetic and environmental stress on the electrotactic movement of animals. Using exposure to chemicals, such as paraquat and tunicamycin, as well as mitochondrial and endoplasmic reticulum (ER) unfolded protein response (UPR) mutants, we demonstrate that chronic stress causes abnormal movement. Additionally, we report that pqe-1 (human RNA exonuclease 1 homolog) is necessary for the maintenance of multiple stress response signaling and electrotaxis behavior of animals. Further, exposure of C. elegans to several environmental stress-inducing conditions revealed that while chronic heat and dietary restriction caused electrotaxis speed deficits due to prolonged stress, daily exercise had a beneficial effect on the animals, likely due to improved muscle health and transient activation of UPR. Overall, these data demonstrate that the electrotaxis behavior of worms is susceptible to cytosolic, mitochondrial, and ER stress, and that multiple stress response pathways contribute to its preservation in the face of stressful stimuli.
format	article
author	Shane K. B. Taylor Muhammad H. Minhas Justin Tong P. Ravi Selvaganapathy Ram K. Mishra Bhagwati P. Gupta
author_facet	Shane K. B. Taylor Muhammad H. Minhas Justin Tong P. Ravi Selvaganapathy Ram K. Mishra Bhagwati P. Gupta
author_sort	Shane K. B. Taylor
title	C. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways
title_short	C. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways
title_full	C. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways
title_fullStr	C. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways
title_full_unstemmed	C. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways
title_sort	c. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1cbde93eba784304bb79dd9f9b099913
work_keys_str_mv	AT shanekbtaylor celeganselectrotaxisbehaviorismodulatedbyheatshockresponseandunfoldedproteinresponsesignalingpathways AT muhammadhminhas celeganselectrotaxisbehaviorismodulatedbyheatshockresponseandunfoldedproteinresponsesignalingpathways AT justintong celeganselectrotaxisbehaviorismodulatedbyheatshockresponseandunfoldedproteinresponsesignalingpathways AT praviselvaganapathy celeganselectrotaxisbehaviorismodulatedbyheatshockresponseandunfoldedproteinresponsesignalingpathways AT ramkmishra celeganselectrotaxisbehaviorismodulatedbyheatshockresponseandunfoldedproteinresponsesignalingpathways AT bhagwatipgupta celeganselectrotaxisbehaviorismodulatedbyheatshockresponseandunfoldedproteinresponsesignalingpathways
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C. elegans electrotaxis behavior is modulated by heat shock response and unfolded protein response signaling pathways

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