Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle

Background: Aberrant metabolism is recognized as a hallmark of cancer, a pillar necessary for cellular proliferation. Regarding bioenergetics (ATP generation), most cancers display a preference not only toward aerobic glycolysis (“Warburg effect”) and glutaminolysis (mitochondrial substrate level-ph...

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Autores principales:	Tomás Duraj, Josefa Carrión-Navarro, Thomas N. Seyfried, Noemí García-Romero, Angel Ayuso-Sacido
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cancer Energy metabolism Glycolysis Oxidative phosphorylation Research design Internal medicine RC31-1245
Acceso en línea:	https://doaj.org/article/1cc69d1a8e794332a08eaed4cf704f72
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spelling	oai:doaj.org-article:1cc69d1a8e794332a08eaed4cf704f722021-11-26T04:28:39ZMetabolic therapy and bioenergetic analysis: The missing piece of the puzzle2212-877810.1016/j.molmet.2021.101389https://doaj.org/article/1cc69d1a8e794332a08eaed4cf704f722021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2212877821002404https://doaj.org/toc/2212-8778Background: Aberrant metabolism is recognized as a hallmark of cancer, a pillar necessary for cellular proliferation. Regarding bioenergetics (ATP generation), most cancers display a preference not only toward aerobic glycolysis (“Warburg effect”) and glutaminolysis (mitochondrial substrate level-phosphorylation) but also toward other metabolites such as lactate, pyruvate, and fat-derived sources. These secondary metabolites can assist in proliferation but cannot fully cover ATP demands. Scope of review: The concept of a static metabolic profile is challenged by instances of heterogeneity and flexibility to meet fuel/anaplerotic demands. Although metabolic therapies are a promising tool to improve therapeutic outcomes, either via pharmacological targets or press-pulse interventions, metabolic plasticity is rarely considered. Lack of bioenergetic analysis in vitro and patient-derived models is hindering translational potential. Here, we review the bioenergetics of cancer and propose a simple analysis of major metabolic pathways, encompassing both affordable and advanced techniques. A comprehensive compendium of Seahorse XF bioenergetic measurements is presented for the first time. Major conclusions: Standardization of principal readouts might help researchers to collect a complete metabolic picture of cancer using the most appropriate methods depending on the sample of interest.Tomás DurajJosefa Carrión-NavarroThomas N. SeyfriedNoemí García-RomeroAngel Ayuso-SacidoElsevierarticleCancerEnergy metabolismGlycolysisOxidative phosphorylationResearch designInternal medicineRC31-1245ENMolecular Metabolism, Vol 54, Iss , Pp 101389- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cancer Energy metabolism Glycolysis Oxidative phosphorylation Research design Internal medicine RC31-1245
spellingShingle	Cancer Energy metabolism Glycolysis Oxidative phosphorylation Research design Internal medicine RC31-1245 Tomás Duraj Josefa Carrión-Navarro Thomas N. Seyfried Noemí García-Romero Angel Ayuso-Sacido Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle
description	Background: Aberrant metabolism is recognized as a hallmark of cancer, a pillar necessary for cellular proliferation. Regarding bioenergetics (ATP generation), most cancers display a preference not only toward aerobic glycolysis (“Warburg effect”) and glutaminolysis (mitochondrial substrate level-phosphorylation) but also toward other metabolites such as lactate, pyruvate, and fat-derived sources. These secondary metabolites can assist in proliferation but cannot fully cover ATP demands. Scope of review: The concept of a static metabolic profile is challenged by instances of heterogeneity and flexibility to meet fuel/anaplerotic demands. Although metabolic therapies are a promising tool to improve therapeutic outcomes, either via pharmacological targets or press-pulse interventions, metabolic plasticity is rarely considered. Lack of bioenergetic analysis in vitro and patient-derived models is hindering translational potential. Here, we review the bioenergetics of cancer and propose a simple analysis of major metabolic pathways, encompassing both affordable and advanced techniques. A comprehensive compendium of Seahorse XF bioenergetic measurements is presented for the first time. Major conclusions: Standardization of principal readouts might help researchers to collect a complete metabolic picture of cancer using the most appropriate methods depending on the sample of interest.
format	article
author	Tomás Duraj Josefa Carrión-Navarro Thomas N. Seyfried Noemí García-Romero Angel Ayuso-Sacido
author_facet	Tomás Duraj Josefa Carrión-Navarro Thomas N. Seyfried Noemí García-Romero Angel Ayuso-Sacido
author_sort	Tomás Duraj
title	Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle
title_short	Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle
title_full	Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle
title_fullStr	Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle
title_full_unstemmed	Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle
title_sort	metabolic therapy and bioenergetic analysis: the missing piece of the puzzle
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/1cc69d1a8e794332a08eaed4cf704f72
work_keys_str_mv	AT tomasduraj metabolictherapyandbioenergeticanalysisthemissingpieceofthepuzzle AT josefacarrionnavarro metabolictherapyandbioenergeticanalysisthemissingpieceofthepuzzle AT thomasnseyfried metabolictherapyandbioenergeticanalysisthemissingpieceofthepuzzle AT noemigarciaromero metabolictherapyandbioenergeticanalysisthemissingpieceofthepuzzle AT angelayusosacido metabolictherapyandbioenergeticanalysisthemissingpieceofthepuzzle
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Metabolic therapy and bioenergetic analysis: The missing piece of the puzzle

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