Mucin dynamics in intestinal bacterial infection.

<h4>Background</h4>Bacterial gastroenteritis causes morbidity and mortality in humans worldwide. Murine Citrobacter rodentium infection is a model for gastroenteritis caused by the human pathogens enteropathogenic Escherichia coli and enterohaemorrhagic E. coli. Mucin glycoproteins are t...

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Autores principales:	Sara K Lindén, Timothy H J Florin, Michael A McGuckin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1cc7e253e5b84c81a2ed47dea3162bd2
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spelling	oai:doaj.org-article:1cc7e253e5b84c81a2ed47dea3162bd22021-11-25T06:18:09ZMucin dynamics in intestinal bacterial infection.1932-620310.1371/journal.pone.0003952https://doaj.org/article/1cc7e253e5b84c81a2ed47dea3162bd22008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19088856/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Bacterial gastroenteritis causes morbidity and mortality in humans worldwide. Murine Citrobacter rodentium infection is a model for gastroenteritis caused by the human pathogens enteropathogenic Escherichia coli and enterohaemorrhagic E. coli. Mucin glycoproteins are the main component of the first barrier that bacteria encounter in the intestinal tract.<h4>Methodology/principal findings</h4>Using Immunohistochemistry, we investigated intestinal expression of mucins (Alcian blue/PAS, Muc1, Muc2, Muc4, Muc5AC, Muc13 and Muc3/17) in healthy and C. rodentium infected mice. The majority of the C. rodentium infected mice developed systemic infection and colitis in the mid and distal colon by day 12. C. rodentium bound to the major secreted mucin, Muc2, in vitro, and high numbers of bacteria were found in secreted MUC2 in infected animals in vivo, indicating that mucins may limit bacterial access to the epithelial surface. In the small intestine, caecum and proximal colon, the mucin expression was similar in infected and non-infected animals. In the distal colonic epithelium, all secreted and cell surface mucins decreased with the exception of the Muc1 cell surface mucin which increased after infection (p<0.05). Similarly, during human infection Salmonella St Paul, Campylobacter jejuni and Clostridium difficile induced MUC1 in the colon.<h4>Conclusion</h4>Major changes in both the cell-surface and secreted mucins occur in response to intestinal infection.Sara K LindénTimothy H J FlorinMichael A McGuckinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e3952 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara K Lindén Timothy H J Florin Michael A McGuckin Mucin dynamics in intestinal bacterial infection.
description	<h4>Background</h4>Bacterial gastroenteritis causes morbidity and mortality in humans worldwide. Murine Citrobacter rodentium infection is a model for gastroenteritis caused by the human pathogens enteropathogenic Escherichia coli and enterohaemorrhagic E. coli. Mucin glycoproteins are the main component of the first barrier that bacteria encounter in the intestinal tract.<h4>Methodology/principal findings</h4>Using Immunohistochemistry, we investigated intestinal expression of mucins (Alcian blue/PAS, Muc1, Muc2, Muc4, Muc5AC, Muc13 and Muc3/17) in healthy and C. rodentium infected mice. The majority of the C. rodentium infected mice developed systemic infection and colitis in the mid and distal colon by day 12. C. rodentium bound to the major secreted mucin, Muc2, in vitro, and high numbers of bacteria were found in secreted MUC2 in infected animals in vivo, indicating that mucins may limit bacterial access to the epithelial surface. In the small intestine, caecum and proximal colon, the mucin expression was similar in infected and non-infected animals. In the distal colonic epithelium, all secreted and cell surface mucins decreased with the exception of the Muc1 cell surface mucin which increased after infection (p<0.05). Similarly, during human infection Salmonella St Paul, Campylobacter jejuni and Clostridium difficile induced MUC1 in the colon.<h4>Conclusion</h4>Major changes in both the cell-surface and secreted mucins occur in response to intestinal infection.
format	article
author	Sara K Lindén Timothy H J Florin Michael A McGuckin
author_facet	Sara K Lindén Timothy H J Florin Michael A McGuckin
author_sort	Sara K Lindén
title	Mucin dynamics in intestinal bacterial infection.
title_short	Mucin dynamics in intestinal bacterial infection.
title_full	Mucin dynamics in intestinal bacterial infection.
title_fullStr	Mucin dynamics in intestinal bacterial infection.
title_full_unstemmed	Mucin dynamics in intestinal bacterial infection.
title_sort	mucin dynamics in intestinal bacterial infection.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/1cc7e253e5b84c81a2ed47dea3162bd2
work_keys_str_mv	AT saraklinden mucindynamicsinintestinalbacterialinfection AT timothyhjflorin mucindynamicsinintestinalbacterialinfection AT michaelamcguckin mucindynamicsinintestinalbacterialinfection
_version_	1718413909870772224

Mucin dynamics in intestinal bacterial infection.

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