Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis

Pyruvate kinase (PYK) controls glycolytic flux. Here, the authors combine biochemical, structural and computational modelling studies to characterize the allosteric mechanisms regulating Mycobacterium tuberculosis PYK activity and show that AMP and glucose-6-phosphate are synergistic allosteric acti...

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Autores principales:	Wenhe Zhong, Liang Cui, Boon Chong Goh, Qixu Cai, Peiying Ho, Yok Hian Chionh, Meng Yuan, Abbas El Sahili, Linda A. Fothergill-Gilmore, Malcolm D. Walkinshaw, Julien Lescar, Peter C. Dedon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1ccbb8a01a5d4d8c9f866502136c19e3
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spelling	oai:doaj.org-article:1ccbb8a01a5d4d8c9f866502136c19e32021-12-02T14:42:07ZAllosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis10.1038/s41467-017-02086-y2041-1723https://doaj.org/article/1ccbb8a01a5d4d8c9f866502136c19e32017-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-02086-yhttps://doaj.org/toc/2041-1723Pyruvate kinase (PYK) controls glycolytic flux. Here, the authors combine biochemical, structural and computational modelling studies to characterize the allosteric mechanisms regulating Mycobacterium tuberculosis PYK activity and show that AMP and glucose-6-phosphate are synergistic allosteric activators of the enzyme.Wenhe ZhongLiang CuiBoon Chong GohQixu CaiPeiying HoYok Hian ChionhMeng YuanAbbas El SahiliLinda A. Fothergill-GilmoreMalcolm D. WalkinshawJulien LescarPeter C. DedonNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Wenhe Zhong Liang Cui Boon Chong Goh Qixu Cai Peiying Ho Yok Hian Chionh Meng Yuan Abbas El Sahili Linda A. Fothergill-Gilmore Malcolm D. Walkinshaw Julien Lescar Peter C. Dedon Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis
description	Pyruvate kinase (PYK) controls glycolytic flux. Here, the authors combine biochemical, structural and computational modelling studies to characterize the allosteric mechanisms regulating Mycobacterium tuberculosis PYK activity and show that AMP and glucose-6-phosphate are synergistic allosteric activators of the enzyme.
format	article
author	Wenhe Zhong Liang Cui Boon Chong Goh Qixu Cai Peiying Ho Yok Hian Chionh Meng Yuan Abbas El Sahili Linda A. Fothergill-Gilmore Malcolm D. Walkinshaw Julien Lescar Peter C. Dedon
author_facet	Wenhe Zhong Liang Cui Boon Chong Goh Qixu Cai Peiying Ho Yok Hian Chionh Meng Yuan Abbas El Sahili Linda A. Fothergill-Gilmore Malcolm D. Walkinshaw Julien Lescar Peter C. Dedon
author_sort	Wenhe Zhong
title	Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis
title_short	Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis
title_full	Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis
title_fullStr	Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis
title_full_unstemmed	Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis
title_sort	allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in mycobacterium tuberculosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1ccbb8a01a5d4d8c9f866502136c19e3
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Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis

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