Fishing for newly synthesized proteins with phosphonate-handles
Here the authors describe PhosID, an enrichment strategy using phosphonate-handles, that combines click chemistry and IMAC-based phospho-enrichment for quantitative proteomics analysis of newly synthesized proteins.
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Nature Portfolio
2020
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oai:doaj.org-article:1ccbb9aaf1384854b862b9b735371b802021-12-02T17:14:22ZFishing for newly synthesized proteins with phosphonate-handles10.1038/s41467-020-17010-02041-1723https://doaj.org/article/1ccbb9aaf1384854b862b9b735371b802020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17010-0https://doaj.org/toc/2041-1723Here the authors describe PhosID, an enrichment strategy using phosphonate-handles, that combines click chemistry and IMAC-based phospho-enrichment for quantitative proteomics analysis of newly synthesized proteins.Fleur KleinpenningBarbara SteigenbergerWei WuAlbert J. R. HeckNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020) |
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DOAJ |
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EN |
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Science Q |
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Science Q Fleur Kleinpenning Barbara Steigenberger Wei Wu Albert J. R. Heck Fishing for newly synthesized proteins with phosphonate-handles |
description |
Here the authors describe PhosID, an enrichment strategy using phosphonate-handles, that combines click chemistry and IMAC-based phospho-enrichment for quantitative proteomics analysis of newly synthesized proteins. |
format |
article |
author |
Fleur Kleinpenning Barbara Steigenberger Wei Wu Albert J. R. Heck |
author_facet |
Fleur Kleinpenning Barbara Steigenberger Wei Wu Albert J. R. Heck |
author_sort |
Fleur Kleinpenning |
title |
Fishing for newly synthesized proteins with phosphonate-handles |
title_short |
Fishing for newly synthesized proteins with phosphonate-handles |
title_full |
Fishing for newly synthesized proteins with phosphonate-handles |
title_fullStr |
Fishing for newly synthesized proteins with phosphonate-handles |
title_full_unstemmed |
Fishing for newly synthesized proteins with phosphonate-handles |
title_sort |
fishing for newly synthesized proteins with phosphonate-handles |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doaj.org/article/1ccbb9aaf1384854b862b9b735371b80 |
work_keys_str_mv |
AT fleurkleinpenning fishingfornewlysynthesizedproteinswithphosphonatehandles AT barbarasteigenberger fishingfornewlysynthesizedproteinswithphosphonatehandles AT weiwu fishingfornewlysynthesizedproteinswithphosphonatehandles AT albertjrheck fishingfornewlysynthesizedproteinswithphosphonatehandles |
_version_ |
1718381307690483712 |