Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)

Abstract Mlph plays a crucial role in regulating skin pigmentation through the melanosome transport process. Although Mlph is a major component involved in melanosome transport, the mechanism that regulates the expression of the Mlph gene has not been identified. In this study, we demonstrate that M...

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Autores principales:	Cheol Hwan Myung, Ji Eun Lee, Chan Song Jo, Jong il Park, Jae Sung Hwang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ccbc7747dd9457da8cede791ced2927
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spelling	oai:doaj.org-article:1ccbc7747dd9457da8cede791ced29272021-12-02T15:10:54ZRegulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)10.1038/s41598-021-96276-w2045-2322https://doaj.org/article/1ccbc7747dd9457da8cede791ced29272021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96276-whttps://doaj.org/toc/2045-2322Abstract Mlph plays a crucial role in regulating skin pigmentation through the melanosome transport process. Although Mlph is a major component involved in melanosome transport, the mechanism that regulates the expression of the Mlph gene has not been identified. In this study, we demonstrate that Mlph expression is regulated by the glucocorticoid receptor (GR). Alteration of GR activity using a specific GR agonist or antagonist only regulated the expression of Mlph among the 3 key melanosome transport proteins. Translocation of GR from the cytosol into the nucleus following Dex treatment was confirmed by separating the cytosol and nuclear fractions and by immunofluorescence staining. In ChIP assays, Dex induced GR binding to the Mlph promoter and we determined that Dex induced the GR binding motif on the Mlph promoter. Our findings contribute to understanding the regulation of Mlph expression and to the novel role of GR in Mlph gene expression.Cheol Hwan MyungJi Eun LeeChan Song JoJong il ParkJae Sung HwangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cheol Hwan Myung Ji Eun Lee Chan Song Jo Jong il Park Jae Sung Hwang Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)
description	Abstract Mlph plays a crucial role in regulating skin pigmentation through the melanosome transport process. Although Mlph is a major component involved in melanosome transport, the mechanism that regulates the expression of the Mlph gene has not been identified. In this study, we demonstrate that Mlph expression is regulated by the glucocorticoid receptor (GR). Alteration of GR activity using a specific GR agonist or antagonist only regulated the expression of Mlph among the 3 key melanosome transport proteins. Translocation of GR from the cytosol into the nucleus following Dex treatment was confirmed by separating the cytosol and nuclear fractions and by immunofluorescence staining. In ChIP assays, Dex induced GR binding to the Mlph promoter and we determined that Dex induced the GR binding motif on the Mlph promoter. Our findings contribute to understanding the regulation of Mlph expression and to the novel role of GR in Mlph gene expression.
format	article
author	Cheol Hwan Myung Ji Eun Lee Chan Song Jo Jong il Park Jae Sung Hwang
author_facet	Cheol Hwan Myung Ji Eun Lee Chan Song Jo Jong il Park Jae Sung Hwang
author_sort	Cheol Hwan Myung
title	Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)
title_short	Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)
title_full	Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)
title_fullStr	Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)
title_full_unstemmed	Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)
title_sort	regulation of melanophilin (mlph) gene expression by the glucocorticoid receptor (gr)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1ccbc7747dd9457da8cede791ced2927
work_keys_str_mv	AT cheolhwanmyung regulationofmelanophilinmlphgeneexpressionbytheglucocorticoidreceptorgr AT jieunlee regulationofmelanophilinmlphgeneexpressionbytheglucocorticoidreceptorgr AT chansongjo regulationofmelanophilinmlphgeneexpressionbytheglucocorticoidreceptorgr AT jongilpark regulationofmelanophilinmlphgeneexpressionbytheglucocorticoidreceptorgr AT jaesunghwang regulationofmelanophilinmlphgeneexpressionbytheglucocorticoidreceptorgr
_version_	1718387615910068224

Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR)

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