Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract

The COVID-19 pandemic has currently created an unprecedented threat to human society and global health. A rapid mass vaccination to create herd immunity against SARS-CoV-2 is a crucial measure to ease the spread of this disease. Here, we investigated the immunogenicity of a SARS-CoV-2 subunit vaccin...

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Autores principales:	Tuksin Jearanaiwitayakul, Suttikarn Apichirapokey, Runglawan Chawengkirttikul, Jitra Limthongkul, Mathurin Seesen, Phissinee Jakaew, Sakalin Trisiriwanich, Sompong Sapsutthipas, Panya Sunintaboon, Sukathida Ubol
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SARS-CoV-2 spike glycoprotein COVID-19 vaccine adjuvant delivery particles peritoneal immunization mucosal immunity Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1ccef95447414f188efbf174b17bb7f1
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spelling	oai:doaj.org-article:1ccef95447414f188efbf174b17bb7f12021-11-25T19:13:18ZPeritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract10.3390/v131122021999-4915https://doaj.org/article/1ccef95447414f188efbf174b17bb7f12021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2202https://doaj.org/toc/1999-4915The COVID-19 pandemic has currently created an unprecedented threat to human society and global health. A rapid mass vaccination to create herd immunity against SARS-CoV-2 is a crucial measure to ease the spread of this disease. Here, we investigated the immunogenicity of a SARS-CoV-2 subunit vaccine candidate, a SARS-CoV-2 spike glycoprotein encapsulated in <i>N</i>,<i>N</i>,<i>N</i>-trimethyl chitosan particles or S-TMC NPs. Upon intraperitoneal immunization, S-TMC NP-immunized mice elicited a stronger systemic antibody response, with neutralizing capacity against SARS-CoV-2, than mice receiving the soluble form of S-glycoprotein. S-TMC NPs were able to stimulate the circulating IgG and IgA as found in SARS-CoV-2-infected patients. In addition, spike-specific T cell responses were drastically activated in S-TMC NP-immunized mice. Surprisingly, administration of S-TMC NPs via the intraperitoneal route also stimulated SARS-CoV-2-specific immune responses in the respiratory tract, which were demonstrated by the presence of high levels of SARS-CoV-2-specific IgG and IgA in the lung homogenates and bronchoalveolar lavages of the immunized mice. We found that peritoneal immunization with spike nanospheres stimulates both systemic and respiratory mucosal immunity.Tuksin JearanaiwitayakulSuttikarn ApichirapokeyRunglawan ChawengkirttikulJitra LimthongkulMathurin SeesenPhissinee JakaewSakalin TrisiriwanichSompong SapsutthipasPanya SunintaboonSukathida UbolMDPI AGarticleSARS-CoV-2 spike glycoproteinCOVID-19 vaccineadjuvant delivery particlesperitoneal immunizationmucosal immunityMicrobiologyQR1-502ENViruses, Vol 13, Iss 2202, p 2202 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 spike glycoprotein COVID-19 vaccine adjuvant delivery particles peritoneal immunization mucosal immunity Microbiology QR1-502
spellingShingle	SARS-CoV-2 spike glycoprotein COVID-19 vaccine adjuvant delivery particles peritoneal immunization mucosal immunity Microbiology QR1-502 Tuksin Jearanaiwitayakul Suttikarn Apichirapokey Runglawan Chawengkirttikul Jitra Limthongkul Mathurin Seesen Phissinee Jakaew Sakalin Trisiriwanich Sompong Sapsutthipas Panya Sunintaboon Sukathida Ubol Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract
description	The COVID-19 pandemic has currently created an unprecedented threat to human society and global health. A rapid mass vaccination to create herd immunity against SARS-CoV-2 is a crucial measure to ease the spread of this disease. Here, we investigated the immunogenicity of a SARS-CoV-2 subunit vaccine candidate, a SARS-CoV-2 spike glycoprotein encapsulated in <i>N</i>,<i>N</i>,<i>N</i>-trimethyl chitosan particles or S-TMC NPs. Upon intraperitoneal immunization, S-TMC NP-immunized mice elicited a stronger systemic antibody response, with neutralizing capacity against SARS-CoV-2, than mice receiving the soluble form of S-glycoprotein. S-TMC NPs were able to stimulate the circulating IgG and IgA as found in SARS-CoV-2-infected patients. In addition, spike-specific T cell responses were drastically activated in S-TMC NP-immunized mice. Surprisingly, administration of S-TMC NPs via the intraperitoneal route also stimulated SARS-CoV-2-specific immune responses in the respiratory tract, which were demonstrated by the presence of high levels of SARS-CoV-2-specific IgG and IgA in the lung homogenates and bronchoalveolar lavages of the immunized mice. We found that peritoneal immunization with spike nanospheres stimulates both systemic and respiratory mucosal immunity.
format	article
author	Tuksin Jearanaiwitayakul Suttikarn Apichirapokey Runglawan Chawengkirttikul Jitra Limthongkul Mathurin Seesen Phissinee Jakaew Sakalin Trisiriwanich Sompong Sapsutthipas Panya Sunintaboon Sukathida Ubol
author_facet	Tuksin Jearanaiwitayakul Suttikarn Apichirapokey Runglawan Chawengkirttikul Jitra Limthongkul Mathurin Seesen Phissinee Jakaew Sakalin Trisiriwanich Sompong Sapsutthipas Panya Sunintaboon Sukathida Ubol
author_sort	Tuksin Jearanaiwitayakul
title	Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract
title_short	Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract
title_full	Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract
title_fullStr	Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract
title_full_unstemmed	Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract
title_sort	peritoneal administration of a subunit vaccine encapsulated in a nanodelivery system not only augments systemic responses against sars-cov-2 but also stimulates responses in the respiratory tract
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1ccef95447414f188efbf174b17bb7f1
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Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract

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