Integrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma

Abstract A metabolomics-based approach to address the molecular mechanism of childhood asthma with immunoglobulin E (IgE) or allergen sensitization related to microbiome in the airways remains lacking. Fifty-three children with lowly sensitized non-atopic asthma (n = 15), highly sensitized atopic as...

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Autores principales:	Chih-Yung Chiu, Mei-Ling Cheng, Meng-Han Chiang, Chia-Jung Wang, Ming-Han Tsai, Gigin Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1cd9764a17434d0183b72be2c4c4d22b
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spelling	oai:doaj.org-article:1cd9764a17434d0183b72be2c4c4d22b2021-12-05T12:16:21ZIntegrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma10.1038/s41598-021-02925-52045-2322https://doaj.org/article/1cd9764a17434d0183b72be2c4c4d22b2021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02925-5https://doaj.org/toc/2045-2322Abstract A metabolomics-based approach to address the molecular mechanism of childhood asthma with immunoglobulin E (IgE) or allergen sensitization related to microbiome in the airways remains lacking. Fifty-three children with lowly sensitized non-atopic asthma (n = 15), highly sensitized atopic asthma (n = 13), and healthy controls (n = 25) were enrolled. Blood metabolomic analysis with 1H-nuclear magnetic resonance (NMR) spectroscopy and airway microbiome composition analysis by bacterial 16S rRNA sequencing were performed. An integrative analysis of their associations with allergen-specific IgE levels for lowly and highly sensitized asthma was also assessed. Four metabolites including tyrosine, isovalerate, glycine, and histidine were uniquely associated with lowly sensitized asthma, whereas one metabolite, acetic acid, was strongly associated with highly sensitized asthma. Metabolites associated with highly sensitized asthma (valine, isobutyric acid, and acetic acid) and lowly sensitized asthma (isovalerate, tyrosine, and histidine) were strongly correlated each other (P < 0.01). Highly sensitized asthma associated metabolites were mainly enriched in pyruvate and acetyl-CoA metabolisms. Metabolites associated with highly sensitized atopic asthma were mostly correlated with microbiota in the airways. Acetic acid, a short-chain fatty acid (SCFA), was negatively correlated with the genus Atopobium (P < 0.01), but positively correlated with the genus Fusobacterium (P < 0.05). In conclusion, metabolomics reveals microbes-related metabolic pathways associated with IgE responses to house dust mite allergens in childhood asthma. A strong correlation of metabolites related to highly sensitized atopic asthma with airway microbiota provides linkages between the host–microbial interactions and asthma endotypes.Chih-Yung ChiuMei-Ling ChengMeng-Han ChiangChia-Jung WangMing-Han TsaiGigin LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chih-Yung Chiu Mei-Ling Cheng Meng-Han Chiang Chia-Jung Wang Ming-Han Tsai Gigin Lin Integrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma
description	Abstract A metabolomics-based approach to address the molecular mechanism of childhood asthma with immunoglobulin E (IgE) or allergen sensitization related to microbiome in the airways remains lacking. Fifty-three children with lowly sensitized non-atopic asthma (n = 15), highly sensitized atopic asthma (n = 13), and healthy controls (n = 25) were enrolled. Blood metabolomic analysis with 1H-nuclear magnetic resonance (NMR) spectroscopy and airway microbiome composition analysis by bacterial 16S rRNA sequencing were performed. An integrative analysis of their associations with allergen-specific IgE levels for lowly and highly sensitized asthma was also assessed. Four metabolites including tyrosine, isovalerate, glycine, and histidine were uniquely associated with lowly sensitized asthma, whereas one metabolite, acetic acid, was strongly associated with highly sensitized asthma. Metabolites associated with highly sensitized asthma (valine, isobutyric acid, and acetic acid) and lowly sensitized asthma (isovalerate, tyrosine, and histidine) were strongly correlated each other (P < 0.01). Highly sensitized asthma associated metabolites were mainly enriched in pyruvate and acetyl-CoA metabolisms. Metabolites associated with highly sensitized atopic asthma were mostly correlated with microbiota in the airways. Acetic acid, a short-chain fatty acid (SCFA), was negatively correlated with the genus Atopobium (P < 0.01), but positively correlated with the genus Fusobacterium (P < 0.05). In conclusion, metabolomics reveals microbes-related metabolic pathways associated with IgE responses to house dust mite allergens in childhood asthma. A strong correlation of metabolites related to highly sensitized atopic asthma with airway microbiota provides linkages between the host–microbial interactions and asthma endotypes.
format	article
author	Chih-Yung Chiu Mei-Ling Cheng Meng-Han Chiang Chia-Jung Wang Ming-Han Tsai Gigin Lin
author_facet	Chih-Yung Chiu Mei-Ling Cheng Meng-Han Chiang Chia-Jung Wang Ming-Han Tsai Gigin Lin
author_sort	Chih-Yung Chiu
title	Integrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma
title_short	Integrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma
title_full	Integrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma
title_fullStr	Integrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma
title_full_unstemmed	Integrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma
title_sort	integrated metabolic and microbial analysis reveals host–microbial interactions in ige-mediated childhood asthma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1cd9764a17434d0183b72be2c4c4d22b
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Integrated metabolic and microbial analysis reveals host–microbial interactions in IgE-mediated childhood asthma

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