Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy

Abstract Microcystis, the dominant species among cyanobacterial blooms, normally forms colonies under natural conditions but exists as single cells or paired cells in axenic laboratory cultures after long-term cultivation. Here, a bloom-forming Microcystis aeruginosa strain CHAOHU 1326 was studied b...

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Autores principales:	Meng Chen, Li-Li Tian, Chong-Yang Ren, Chun-Yang Xu, Yi-Ying Wang, Li Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1cdc18c2e503432bafbb5df3606555de
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spelling	oai:doaj.org-article:1cdc18c2e503432bafbb5df3606555de2021-12-02T16:08:43ZExtracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy10.1038/s41598-018-37398-62045-2322https://doaj.org/article/1cdc18c2e503432bafbb5df3606555de2019-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-37398-6https://doaj.org/toc/2045-2322Abstract Microcystis, the dominant species among cyanobacterial blooms, normally forms colonies under natural conditions but exists as single cells or paired cells in axenic laboratory cultures after long-term cultivation. Here, a bloom-forming Microcystis aeruginosa strain CHAOHU 1326 was studied because it presents a colonial morphology and grows on the water surface during axenic laboratory culturing. We first examined the morphological features of strain CHAOHU 1326 and three other unicellular M. aeruginosa strains FACHB-925, FACHB-940, and FACHB-975 cultured under the same conditions by scanning and transmission electron microscopy. Then, we compared the extracellular polysaccharide (EPS)-producing ability of colonial strain CHAOHU 1326 to that of the three unicellular M. aeruginosa strains, and found that strain CHAOHU 1326 produced a higher amount of EPS than the other strains during growth. Moreover, based on genome sequencing, multiple gene clusters implicated in EPS biosynthesis and a cluster of 12 genes predicted to be involved in gas vesicle synthesis in strain CHAOHU 1326 were detected. These predicted genes were all functional and expressed in M. aeruginosa CHAOHU 1326 as determined by reverse transcription PCR. These findings provide a physiological and genetic basis to better understand colony formation and buoyancy control during M. aeruginosa blooming.Meng ChenLi-Li TianChong-Yang RenChun-Yang XuYi-Ying WangLi LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meng Chen Li-Li Tian Chong-Yang Ren Chun-Yang Xu Yi-Ying Wang Li Li Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy
description	Abstract Microcystis, the dominant species among cyanobacterial blooms, normally forms colonies under natural conditions but exists as single cells or paired cells in axenic laboratory cultures after long-term cultivation. Here, a bloom-forming Microcystis aeruginosa strain CHAOHU 1326 was studied because it presents a colonial morphology and grows on the water surface during axenic laboratory culturing. We first examined the morphological features of strain CHAOHU 1326 and three other unicellular M. aeruginosa strains FACHB-925, FACHB-940, and FACHB-975 cultured under the same conditions by scanning and transmission electron microscopy. Then, we compared the extracellular polysaccharide (EPS)-producing ability of colonial strain CHAOHU 1326 to that of the three unicellular M. aeruginosa strains, and found that strain CHAOHU 1326 produced a higher amount of EPS than the other strains during growth. Moreover, based on genome sequencing, multiple gene clusters implicated in EPS biosynthesis and a cluster of 12 genes predicted to be involved in gas vesicle synthesis in strain CHAOHU 1326 were detected. These predicted genes were all functional and expressed in M. aeruginosa CHAOHU 1326 as determined by reverse transcription PCR. These findings provide a physiological and genetic basis to better understand colony formation and buoyancy control during M. aeruginosa blooming.
format	article
author	Meng Chen Li-Li Tian Chong-Yang Ren Chun-Yang Xu Yi-Ying Wang Li Li
author_facet	Meng Chen Li-Li Tian Chong-Yang Ren Chun-Yang Xu Yi-Ying Wang Li Li
author_sort	Meng Chen
title	Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy
title_short	Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy
title_full	Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy
title_fullStr	Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy
title_full_unstemmed	Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy
title_sort	extracellular polysaccharide synthesis in a bloom-forming strain of microcystis aeruginosa: implications for colonization and buoyancy
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/1cdc18c2e503432bafbb5df3606555de
work_keys_str_mv	AT mengchen extracellularpolysaccharidesynthesisinabloomformingstrainofmicrocystisaeruginosaimplicationsforcolonizationandbuoyancy AT lilitian extracellularpolysaccharidesynthesisinabloomformingstrainofmicrocystisaeruginosaimplicationsforcolonizationandbuoyancy AT chongyangren extracellularpolysaccharidesynthesisinabloomformingstrainofmicrocystisaeruginosaimplicationsforcolonizationandbuoyancy AT chunyangxu extracellularpolysaccharidesynthesisinabloomformingstrainofmicrocystisaeruginosaimplicationsforcolonizationandbuoyancy AT yiyingwang extracellularpolysaccharidesynthesisinabloomformingstrainofmicrocystisaeruginosaimplicationsforcolonizationandbuoyancy AT lili extracellularpolysaccharidesynthesisinabloomformingstrainofmicrocystisaeruginosaimplicationsforcolonizationandbuoyancy
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Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy

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