EMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway

Abstract EMI Domain Containing 1 (EMID1) was identified as a potential candidate metastasis-promoting gene. We sought to clarify the molecular function of EMID1 and the protein expression. Overexpression and knockdown studies using mouse tumor cell lines identified two novel functions of EMID1: intr...

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Autores principales:	Takuya Kawata, Koji Muramatsu, Namiko Shishito, Naoki Ichikawa-Tomikawa, Takuma Oishi, Yuko Kakuda, Yasuto Akiyama, Ken Yamaguchi, Michiie Sakamoto, Takashi Sugino
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ce32b0292a04ddf90fc8ea5315239c5
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spelling	oai:doaj.org-article:1ce32b0292a04ddf90fc8ea5315239c52021-12-02T18:50:44ZEMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway10.1038/s41598-021-96006-22045-2322https://doaj.org/article/1ce32b0292a04ddf90fc8ea5315239c52021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96006-2https://doaj.org/toc/2045-2322Abstract EMI Domain Containing 1 (EMID1) was identified as a potential candidate metastasis-promoting gene. We sought to clarify the molecular function of EMID1 and the protein expression. Overexpression and knockdown studies using mouse tumor cell lines identified two novel functions of EMID1: intracellular signaling involving enhancement of cell growth via cell cycle promotion and suppression of cell motility, and inhibition of cell–matrix adhesion by extracellularly secreted EMID1. EMID1 deposited on the culture dish induced self-detachment of cells that overexpressed the protein and inhibited adhesion of additionally seeded cells. This multifunctional property involving both intracellular signaling and the extracellular matrix suggests that EMID1 may be a matricellular proteins. Expression analysis using immunohistochemical staining revealed expression of EMID1 that was limited to chief cells of the gastric fundic gland and β cells of the pancreatic islets in normal adult human tissues, implying cell-specific functions of this molecule. In addition, increased expression of EMID1 protein detected in some cases of human cancers implies that EMID1 might be a new therapeutic target for cancer treatment.Takuya KawataKoji MuramatsuNamiko ShishitoNaoki Ichikawa-TomikawaTakuma OishiYuko KakudaYasuto AkiyamaKen YamaguchiMichiie SakamotoTakashi SuginoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takuya Kawata Koji Muramatsu Namiko Shishito Naoki Ichikawa-Tomikawa Takuma Oishi Yuko Kakuda Yasuto Akiyama Ken Yamaguchi Michiie Sakamoto Takashi Sugino EMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway
description	Abstract EMI Domain Containing 1 (EMID1) was identified as a potential candidate metastasis-promoting gene. We sought to clarify the molecular function of EMID1 and the protein expression. Overexpression and knockdown studies using mouse tumor cell lines identified two novel functions of EMID1: intracellular signaling involving enhancement of cell growth via cell cycle promotion and suppression of cell motility, and inhibition of cell–matrix adhesion by extracellularly secreted EMID1. EMID1 deposited on the culture dish induced self-detachment of cells that overexpressed the protein and inhibited adhesion of additionally seeded cells. This multifunctional property involving both intracellular signaling and the extracellular matrix suggests that EMID1 may be a matricellular proteins. Expression analysis using immunohistochemical staining revealed expression of EMID1 that was limited to chief cells of the gastric fundic gland and β cells of the pancreatic islets in normal adult human tissues, implying cell-specific functions of this molecule. In addition, increased expression of EMID1 protein detected in some cases of human cancers implies that EMID1 might be a new therapeutic target for cancer treatment.
format	article
author	Takuya Kawata Koji Muramatsu Namiko Shishito Naoki Ichikawa-Tomikawa Takuma Oishi Yuko Kakuda Yasuto Akiyama Ken Yamaguchi Michiie Sakamoto Takashi Sugino
author_facet	Takuya Kawata Koji Muramatsu Namiko Shishito Naoki Ichikawa-Tomikawa Takuma Oishi Yuko Kakuda Yasuto Akiyama Ken Yamaguchi Michiie Sakamoto Takashi Sugino
author_sort	Takuya Kawata
title	EMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway
title_short	EMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway
title_full	EMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway
title_fullStr	EMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway
title_full_unstemmed	EMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway
title_sort	emid1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1ce32b0292a04ddf90fc8ea5315239c5
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EMID1, a multifunctional molecule identified in a murine model for the invasion independent metastasis pathway

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