Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects

Jian Liu,1,2,* Jinfei Hou,1,2,* Shaokai Liu,1,2 Jialun Li,1,2 Muran Zhou,1,2 Jiaming Sun,1,2 Rongrong Wang1,2 1Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China; 2Wuhan Clinical Re...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liu J, Hou J, Liu S, Li J, Zhou M, Sun J, Wang R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	abdominal wall defects graphene oxide chitosan n-acetylcysteine double layer electrospinning Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1ce333532f18402ea57ccc113a97dc21
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1ce333532f18402ea57ccc113a97dc21
record_format	dspace
spelling	oai:doaj.org-article:1ce333532f18402ea57ccc113a97dc212021-12-02T18:41:14ZGraphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects1178-2013https://doaj.org/article/1ce333532f18402ea57ccc113a97dc212021-06-01T00:00:00Zhttps://www.dovepress.com/graphene-oxide-functionalized-double-layered-patch-with-anti-adhesion--peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Jian Liu,1,2,* Jinfei Hou,1,2,* Shaokai Liu,1,2 Jialun Li,1,2 Muran Zhou,1,2 Jiaming Sun,1,2 Rongrong Wang1,2 1Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China; 2Wuhan Clinical Research Center for Superficial Organ Reconstruction, Wuhan, 430022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Rongrong Wang; Jiaming SunDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, People’s Republic of ChinaTel +86-18995511113; Tel +86-13986246496Fax +86-027-85726240Email wangrongrong1988@126.com; 2004xh0801@hust.edu.cnBackground: Effective repair of full-thickness abdominal wall defects requires a patch with sufficient mechanical strength and anti-adhesion characteristics to avoid the formation of hernias and intra-abdominal complications such as intestinal obstruction and fistula. However, patches made from polymers or bio-derived materials may not meet these requirements and lack the bionic characteristics of the abdominal wall.Materials and Methods: In this study, we report a consecutive electrospun method for preparing a double-layer structured nanofiber membrane (GO-PCL/CS-PCL) using polycaprolactone (PCL), graphene oxide (GO) and chitosan (CS). To expand the bio-functions (angiogenesis/reducing reactive oxygen species) of the patch (GO-PCL/NAC-CS-PCL), N-acetylcysteine (NAC) was loaded for the repair of full-thickness abdominal wall defects (2× 1.5cm) in rat model.Results: The double-layered patch (GO-PCL/NAC-CS-PCL) showed excellent mechanical strength and biocompatibility. After 2 months, rats treated with the patch exhibited the desired repair effect with no hernia formation, less adhesion (adhesion score: 1.50± 0.50, P< 0.001) and more collagen deposition (percentage of collagen deposition: 34.94%± 3.31%, P< 0.001).Conclusion: The double-layered nanomembranes presented in this study have good anti-hernia and anti-adhesion effects, as well as improve the microenvironment in vivo. It, therefore, holds good prospects for the repair of abdominal wall defects and provides a promising key as a postoperative anti-adhesion agent.Keywords: abdominal wall defects, graphene oxide, chitosan, N-acetylcysteine, double layer, electrospinningLiu JHou JLiu SLi JZhou MSun JWang RDove Medical Pressarticleabdominal wall defectsgraphene oxidechitosann-acetylcysteinedouble layerelectrospinningMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3803-3818 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	abdominal wall defects graphene oxide chitosan n-acetylcysteine double layer electrospinning Medicine (General) R5-920
spellingShingle	abdominal wall defects graphene oxide chitosan n-acetylcysteine double layer electrospinning Medicine (General) R5-920 Liu J Hou J Liu S Li J Zhou M Sun J Wang R Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects
description	Jian Liu,1,2,* Jinfei Hou,1,2,* Shaokai Liu,1,2 Jialun Li,1,2 Muran Zhou,1,2 Jiaming Sun,1,2 Rongrong Wang1,2 1Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China; 2Wuhan Clinical Research Center for Superficial Organ Reconstruction, Wuhan, 430022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Rongrong Wang; Jiaming SunDepartment of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan, 430022, People’s Republic of ChinaTel +86-18995511113; Tel +86-13986246496Fax +86-027-85726240Email wangrongrong1988@126.com; 2004xh0801@hust.edu.cnBackground: Effective repair of full-thickness abdominal wall defects requires a patch with sufficient mechanical strength and anti-adhesion characteristics to avoid the formation of hernias and intra-abdominal complications such as intestinal obstruction and fistula. However, patches made from polymers or bio-derived materials may not meet these requirements and lack the bionic characteristics of the abdominal wall.Materials and Methods: In this study, we report a consecutive electrospun method for preparing a double-layer structured nanofiber membrane (GO-PCL/CS-PCL) using polycaprolactone (PCL), graphene oxide (GO) and chitosan (CS). To expand the bio-functions (angiogenesis/reducing reactive oxygen species) of the patch (GO-PCL/NAC-CS-PCL), N-acetylcysteine (NAC) was loaded for the repair of full-thickness abdominal wall defects (2× 1.5cm) in rat model.Results: The double-layered patch (GO-PCL/NAC-CS-PCL) showed excellent mechanical strength and biocompatibility. After 2 months, rats treated with the patch exhibited the desired repair effect with no hernia formation, less adhesion (adhesion score: 1.50± 0.50, P< 0.001) and more collagen deposition (percentage of collagen deposition: 34.94%± 3.31%, P< 0.001).Conclusion: The double-layered nanomembranes presented in this study have good anti-hernia and anti-adhesion effects, as well as improve the microenvironment in vivo. It, therefore, holds good prospects for the repair of abdominal wall defects and provides a promising key as a postoperative anti-adhesion agent.Keywords: abdominal wall defects, graphene oxide, chitosan, N-acetylcysteine, double layer, electrospinning
format	article
author	Liu J Hou J Liu S Li J Zhou M Sun J Wang R
author_facet	Liu J Hou J Liu S Li J Zhou M Sun J Wang R
author_sort	Liu J
title	Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects
title_short	Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects
title_full	Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects
title_fullStr	Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects
title_full_unstemmed	Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects
title_sort	graphene oxide functionalized double-layered patch with anti-adhesion ability for abdominal wall defects
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/1ce333532f18402ea57ccc113a97dc21
work_keys_str_mv	AT liuj grapheneoxidefunctionalizeddoublelayeredpatchwithantiadhesionabilityforabdominalwalldefects AT houj grapheneoxidefunctionalizeddoublelayeredpatchwithantiadhesionabilityforabdominalwalldefects AT lius grapheneoxidefunctionalizeddoublelayeredpatchwithantiadhesionabilityforabdominalwalldefects AT lij grapheneoxidefunctionalizeddoublelayeredpatchwithantiadhesionabilityforabdominalwalldefects AT zhoum grapheneoxidefunctionalizeddoublelayeredpatchwithantiadhesionabilityforabdominalwalldefects AT sunj grapheneoxidefunctionalizeddoublelayeredpatchwithantiadhesionabilityforabdominalwalldefects AT wangr grapheneoxidefunctionalizeddoublelayeredpatchwithantiadhesionabilityforabdominalwalldefects
_version_	1718377726711169024

Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects

Ejemplares similares