Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples

Highly selective multifunctional magnetic nanoparticles containing a thermoresponsive polymer shell were developed and used in the sample pretreatment of urine for the assessment of lysozymuria in leukemia patients. Crosslinked poly(N-isopropylacrylamide-co-acrylic acid-co-N-tert-butylacrylamide) wa...

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Autores principales:	Marwa A. Ahmed, Júlia Erdőssy, Viola Horvath
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	thermoresponsive polymer magnetic nanoparticle lysozyme bioanalytical method validation Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1ce43f8d2dea4bc09ab41b5a7b6d643e
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spelling	oai:doaj.org-article:1ce43f8d2dea4bc09ab41b5a7b6d643e2021-11-25T18:31:44ZTemperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples10.3390/nano111130152079-4991https://doaj.org/article/1ce43f8d2dea4bc09ab41b5a7b6d643e2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/3015https://doaj.org/toc/2079-4991Highly selective multifunctional magnetic nanoparticles containing a thermoresponsive polymer shell were developed and used in the sample pretreatment of urine for the assessment of lysozymuria in leukemia patients. Crosslinked poly(N-isopropylacrylamide-co-acrylic acid-co-N-tert-butylacrylamide) was grown onto silica-coated magnetic nanoparticles by reversible addition fragmentation chain transfer (RAFT) polymerization. The lysozyme binding property of the nanoparticles was investigated as a function of time, protein concentration, pH, ionic strength and temperature and their selectivity was assessed against other proteins. High-abundant proteins, like human serum albumin and γ-globulins did not interfere with the binding of lysozyme even at elevated concentrations characteristic of proteinuria. A sample cleanup procedure for urine samples has been developed utilizing the thermocontrollable protein binding ability of the nanoparticles. Method validation was carried out according to current bioanalytical method validation guidelines. The method was highly selective, and the calibration was linear in the 25 to 1000 µg/mL concentration range, relevant in the diagnosis of monocytic and myelomonocytic leukemia. Intra- and inter-day precision values ranged from 2.24 to 8.20% and 1.08 to 5.04%, respectively. Intra-day accuracies were between 89.9 and 117.6%, while inter-day accuracies were in the 88.8 to 111.0% range. The average recovery was 94.1 ± 8.1%. Analysis of unknown urine samples in comparison with a well-established reference method revealed very good correlation between the results, indicating that the new nanoparticle-based method has high potential in the diagnosis of lysozymuria.Marwa A. AhmedJúlia ErdőssyViola HorvathMDPI AGarticlethermoresponsive polymermagnetic nanoparticlelysozymebioanalytical method validationChemistryQD1-999ENNanomaterials, Vol 11, Iss 3015, p 3015 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	thermoresponsive polymer magnetic nanoparticle lysozyme bioanalytical method validation Chemistry QD1-999
spellingShingle	thermoresponsive polymer magnetic nanoparticle lysozyme bioanalytical method validation Chemistry QD1-999 Marwa A. Ahmed Júlia Erdőssy Viola Horvath Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples
description	Highly selective multifunctional magnetic nanoparticles containing a thermoresponsive polymer shell were developed and used in the sample pretreatment of urine for the assessment of lysozymuria in leukemia patients. Crosslinked poly(N-isopropylacrylamide-co-acrylic acid-co-N-tert-butylacrylamide) was grown onto silica-coated magnetic nanoparticles by reversible addition fragmentation chain transfer (RAFT) polymerization. The lysozyme binding property of the nanoparticles was investigated as a function of time, protein concentration, pH, ionic strength and temperature and their selectivity was assessed against other proteins. High-abundant proteins, like human serum albumin and γ-globulins did not interfere with the binding of lysozyme even at elevated concentrations characteristic of proteinuria. A sample cleanup procedure for urine samples has been developed utilizing the thermocontrollable protein binding ability of the nanoparticles. Method validation was carried out according to current bioanalytical method validation guidelines. The method was highly selective, and the calibration was linear in the 25 to 1000 µg/mL concentration range, relevant in the diagnosis of monocytic and myelomonocytic leukemia. Intra- and inter-day precision values ranged from 2.24 to 8.20% and 1.08 to 5.04%, respectively. Intra-day accuracies were between 89.9 and 117.6%, while inter-day accuracies were in the 88.8 to 111.0% range. The average recovery was 94.1 ± 8.1%. Analysis of unknown urine samples in comparison with a well-established reference method revealed very good correlation between the results, indicating that the new nanoparticle-based method has high potential in the diagnosis of lysozymuria.
format	article
author	Marwa A. Ahmed Júlia Erdőssy Viola Horvath
author_facet	Marwa A. Ahmed Júlia Erdőssy Viola Horvath
author_sort	Marwa A. Ahmed
title	Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples
title_short	Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples
title_full	Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples
title_fullStr	Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples
title_full_unstemmed	Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples
title_sort	temperature-responsive magnetic nanoparticles for bioanalysis of lysozyme in urine samples
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1ce43f8d2dea4bc09ab41b5a7b6d643e
work_keys_str_mv	AT marwaaahmed temperatureresponsivemagneticnanoparticlesforbioanalysisoflysozymeinurinesamples AT juliaerdossy temperatureresponsivemagneticnanoparticlesforbioanalysisoflysozymeinurinesamples AT violahorvath temperatureresponsivemagneticnanoparticlesforbioanalysisoflysozymeinurinesamples
_version_	1718411001813008384

Temperature-Responsive Magnetic Nanoparticles for Bioanalysis of Lysozyme in Urine Samples

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