Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer

In this study, we present a simple approach for developing a biocompatible polymer scaffold with a honeycomb-like micropattern. We aimed to combine a plasma treatment of fluorinated ethylene propylene (FEP) substrate with an improved phase separation technique. The plasma exposure served for modific...

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Autores principales:	Klaudia Hurtuková, Veronika Juřicová, Klára Fajstavrová, Dominik Fajstavr, Nikola Slepičková Kasálková, Silvie Rimpelová, Václav Švorčík, Petr Slepička
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polymer plasma modification PMMA material morphology honeycomb-like pattern cell growth Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/1cecbe5e40254df99cc76d94df5c3741
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spelling	oai:doaj.org-article:1cecbe5e40254df99cc76d94df5c37412021-11-11T18:43:04ZCytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer10.3390/polym132136632073-4360https://doaj.org/article/1cecbe5e40254df99cc76d94df5c37412021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3663https://doaj.org/toc/2073-4360In this study, we present a simple approach for developing a biocompatible polymer scaffold with a honeycomb-like micropattern. We aimed to combine a plasma treatment of fluorinated ethylene propylene (FEP) substrate with an improved phase separation technique. The plasma exposure served for modification of the polymer surface properties, such as roughness, surface chemistry, and wettability. The treated FEP substrate was applied for the growth of a honeycomb-like pattern from a solution of polymethyl methacrylate (PMMA). The properties of the pattern were strongly dependent on the conditions of plasma exposure of the FEP substrate. The physico-chemical properties of the prepared pattern, such as changes in wettability, aging, morphology, and surface chemistry, were determined. Further, we have examined the cellular response of human osteoblasts (U-2 OS) on the modified substrates. The micropattern prepared with a selected combination of surface activation and amount of PMMA for honeycomb construction showed a positive effect on U-2 OS cell adhesion and proliferation. Samples with higher PMMA content (3 and 4 g) formed more periodic hexagonal structures on the surface compared to its lower amount (1 and 2 g), which led to a significant increase in the pattern cytocompatibility compared to pristine or plasma-treated FEP.Klaudia HurtukováVeronika JuřicováKlára FajstavrováDominik FajstavrNikola Slepičková KasálkováSilvie RimpelováVáclav ŠvorčíkPetr SlepičkaMDPI AGarticlepolymerplasma modificationPMMAmaterial morphologyhoneycomb-like patterncell growthOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3663, p 3663 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polymer plasma modification PMMA material morphology honeycomb-like pattern cell growth Organic chemistry QD241-441
spellingShingle	polymer plasma modification PMMA material morphology honeycomb-like pattern cell growth Organic chemistry QD241-441 Klaudia Hurtuková Veronika Juřicová Klára Fajstavrová Dominik Fajstavr Nikola Slepičková Kasálková Silvie Rimpelová Václav Švorčík Petr Slepička Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer
description	In this study, we present a simple approach for developing a biocompatible polymer scaffold with a honeycomb-like micropattern. We aimed to combine a plasma treatment of fluorinated ethylene propylene (FEP) substrate with an improved phase separation technique. The plasma exposure served for modification of the polymer surface properties, such as roughness, surface chemistry, and wettability. The treated FEP substrate was applied for the growth of a honeycomb-like pattern from a solution of polymethyl methacrylate (PMMA). The properties of the pattern were strongly dependent on the conditions of plasma exposure of the FEP substrate. The physico-chemical properties of the prepared pattern, such as changes in wettability, aging, morphology, and surface chemistry, were determined. Further, we have examined the cellular response of human osteoblasts (U-2 OS) on the modified substrates. The micropattern prepared with a selected combination of surface activation and amount of PMMA for honeycomb construction showed a positive effect on U-2 OS cell adhesion and proliferation. Samples with higher PMMA content (3 and 4 g) formed more periodic hexagonal structures on the surface compared to its lower amount (1 and 2 g), which led to a significant increase in the pattern cytocompatibility compared to pristine or plasma-treated FEP.
format	article
author	Klaudia Hurtuková Veronika Juřicová Klára Fajstavrová Dominik Fajstavr Nikola Slepičková Kasálková Silvie Rimpelová Václav Švorčík Petr Slepička
author_facet	Klaudia Hurtuková Veronika Juřicová Klára Fajstavrová Dominik Fajstavr Nikola Slepičková Kasálková Silvie Rimpelová Václav Švorčík Petr Slepička
author_sort	Klaudia Hurtuková
title	Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer
title_short	Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer
title_full	Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer
title_fullStr	Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer
title_full_unstemmed	Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer
title_sort	cytocompatibility of polymethyl methacrylate honeycomb-like pattern on perfluorinated polymer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1cecbe5e40254df99cc76d94df5c3741
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Cytocompatibility of Polymethyl Methacrylate Honeycomb-like Pattern on Perfluorinated Polymer

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