Integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice

Abstract Leaf senescence is one of the most precisely modulated developmental process and affects various agronomic traits of rice. Anti-senescence rice varieties are important for breeding application. However, little is known about the mechanisms underlying the metabolic regulatory process of leaf...

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Autores principales:	Jiao Xue, Dongbai Lu, Shiguang Wang, Zhanhua Lu, Wei Liu, Xiaofei Wang, Zhiqiang Fang, Xiuying He
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1cede78019d646bda260b81d79d7ea47
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spelling	oai:doaj.org-article:1cede78019d646bda260b81d79d7ea472021-12-02T15:22:57ZIntegrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice10.1038/s41598-021-93532-x2045-2322https://doaj.org/article/1cede78019d646bda260b81d79d7ea472021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93532-xhttps://doaj.org/toc/2045-2322Abstract Leaf senescence is one of the most precisely modulated developmental process and affects various agronomic traits of rice. Anti-senescence rice varieties are important for breeding application. However, little is known about the mechanisms underlying the metabolic regulatory process of leaf senescence in rice. In this study, we performed transcriptomic and metabolomic analyses of the flag leaves in Yuenong Simiao (YN) and YB, two indica rice cultivars that differ in terms of their leaf senescence. We found 8524 genes/204 metabolites were differentially expressed/accumulated in YN at 30 days after flowering (DAF) compared to 0 DAF, and 8799 genes/205 metabolites were differentially expressed in YB at 30 DAF compared to 0 DAF. Integrative analyses showed that a set of genes and metabolites involved in flavonoid pathway were significantly enriched. We identified that relative accumulation of PHENYLALANINE AMMONIA-LYASE (PAL), CINNAMATE 4-HYDROXYLASE (C4H), 4-COUMAROYL-COA LIGASE (4CL), CHALCONE SYNTHASE (CHS) and CHALCONE ISOMERASE (CHI) in YN30/0 was higher than that in YB30/0. Three flavonoid derivatives, including phloretin, luteolin and eriodictyol, showed lower abundances in YB than in YN at 30 DAF. We further revealed a MYB transcription factor, which is encoded by OsR498G0101613100 gene, could suppress the expression of CHI and CHS. Our results suggested a comprehensive analysis of leaf senescence in a view of transcriptome and metabolome and would contribute to exploring the molecular mechanism of leaf senescence in rice.Jiao XueDongbai LuShiguang WangZhanhua LuWei LiuXiaofei WangZhiqiang FangXiuying HeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiao Xue Dongbai Lu Shiguang Wang Zhanhua Lu Wei Liu Xiaofei Wang Zhiqiang Fang Xiuying He Integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice
description	Abstract Leaf senescence is one of the most precisely modulated developmental process and affects various agronomic traits of rice. Anti-senescence rice varieties are important for breeding application. However, little is known about the mechanisms underlying the metabolic regulatory process of leaf senescence in rice. In this study, we performed transcriptomic and metabolomic analyses of the flag leaves in Yuenong Simiao (YN) and YB, two indica rice cultivars that differ in terms of their leaf senescence. We found 8524 genes/204 metabolites were differentially expressed/accumulated in YN at 30 days after flowering (DAF) compared to 0 DAF, and 8799 genes/205 metabolites were differentially expressed in YB at 30 DAF compared to 0 DAF. Integrative analyses showed that a set of genes and metabolites involved in flavonoid pathway were significantly enriched. We identified that relative accumulation of PHENYLALANINE AMMONIA-LYASE (PAL), CINNAMATE 4-HYDROXYLASE (C4H), 4-COUMAROYL-COA LIGASE (4CL), CHALCONE SYNTHASE (CHS) and CHALCONE ISOMERASE (CHI) in YN30/0 was higher than that in YB30/0. Three flavonoid derivatives, including phloretin, luteolin and eriodictyol, showed lower abundances in YB than in YN at 30 DAF. We further revealed a MYB transcription factor, which is encoded by OsR498G0101613100 gene, could suppress the expression of CHI and CHS. Our results suggested a comprehensive analysis of leaf senescence in a view of transcriptome and metabolome and would contribute to exploring the molecular mechanism of leaf senescence in rice.
format	article
author	Jiao Xue Dongbai Lu Shiguang Wang Zhanhua Lu Wei Liu Xiaofei Wang Zhiqiang Fang Xiuying He
author_facet	Jiao Xue Dongbai Lu Shiguang Wang Zhanhua Lu Wei Liu Xiaofei Wang Zhiqiang Fang Xiuying He
author_sort	Jiao Xue
title	Integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice
title_short	Integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice
title_full	Integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice
title_fullStr	Integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice
title_full_unstemmed	Integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice
title_sort	integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1cede78019d646bda260b81d79d7ea47
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Integrated transcriptomic and metabolomic analysis provides insight into the regulation of leaf senescence in rice

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