Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome

BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined...

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Autores principales:	Chaojie Yu, Xinli Zhan, Tuo Liang, Liyi Chen, Zide Zhang, Jie Jiang, Jiang Xue, Jiarui Chen, Chong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1cf0c3b335304580915fad2224613ceb
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spelling	oai:doaj.org-article:1cf0c3b335304580915fad2224613ceb2021-11-22T11:37:03ZMechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome1664-322410.3389/fimmu.2021.572592https://doaj.org/article/1cf0c3b335304580915fad2224613ceb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.572592/fullhttps://doaj.org/toc/1664-3224BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway.Chaojie YuXinli ZhanTuo LiangLiyi ChenZide ZhangJie JiangJiang XueJiarui ChenChong LiuFrontiers Media S.A.articleankylosing spondylitisproteomicsbioinformaticsmyeloperoxidasephagosomeImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome Immunologic diseases. Allergy RC581-607
spellingShingle	ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome Immunologic diseases. Allergy RC581-607 Chaojie Yu Xinli Zhan Tuo Liang Liyi Chen Zide Zhang Jie Jiang Jiang Xue Jiarui Chen Chong Liu Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
description	BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway.
format	article
author	Chaojie Yu Xinli Zhan Tuo Liang Liyi Chen Zide Zhang Jie Jiang Jiang Xue Jiarui Chen Chong Liu
author_facet	Chaojie Yu Xinli Zhan Tuo Liang Liyi Chen Zide Zhang Jie Jiang Jiang Xue Jiarui Chen Chong Liu
author_sort	Chaojie Yu
title	Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
title_short	Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
title_full	Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
title_fullStr	Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
title_full_unstemmed	Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
title_sort	mechanism of hip arthropathy in ankylosing spondylitis: abnormal myeloperoxidase and phagosome
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1cf0c3b335304580915fad2224613ceb
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Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome

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