DNA Microarrays in the Undergraduate Microbiology Lab: Experimentation and Handling Large Datasets in as Few as Six Weeks

DNA microarrays have significantly impacted the study of gene expression on a genome-wide level but also have forced a more global consideration of research questions. As such, it has become critical to introduce undergraduate students to genomics approaches to research. A challenge with performing...

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Autor principal:	David B. Kushner
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2007
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1cf8211273b3431c930de97a7bbb26db
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spelling	oai:doaj.org-article:1cf8211273b3431c930de97a7bbb26db2021-11-15T15:03:04ZDNA Microarrays in the Undergraduate Microbiology Lab: Experimentation and Handling Large Datasets in as Few as Six Weeks10.1128/jmbe.8.1.3-12.20071935-78851935-7877https://doaj.org/article/1cf8211273b3431c930de97a7bbb26db2007-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.8.1.3-12.2007https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885DNA microarrays have significantly impacted the study of gene expression on a genome-wide level but also have forced a more global consideration of research questions. As such, it has become critical to introduce undergraduate students to genomics approaches to research. A challenge with performing a DNA microarray experiment in the teaching lab is determining the time required for the study and how to handle the voluminous data generated. At an unexpectedly low cost, a 6-week, project-based lab module has been developed that provides 3 weeks for wet lab (hands-on work with the DNA microarrays) and 3 weeks for dry lab (analyzing data, using databases to help with data analysis, and considering the meaning of data within the large dataset). Options exist for extending the number of weeks dedicated to the project, but 6 weeks is sufficient for providing an introduction to both experimental genomics and data analysis. Students indicate that being able to both perform array experiments and thoroughly analyze data enriches their understanding of genomics and the complexity of biological systems.David B. KushnerAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 8, Iss 1, Pp 3-12 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 David B. Kushner DNA Microarrays in the Undergraduate Microbiology Lab: Experimentation and Handling Large Datasets in as Few as Six Weeks
description	DNA microarrays have significantly impacted the study of gene expression on a genome-wide level but also have forced a more global consideration of research questions. As such, it has become critical to introduce undergraduate students to genomics approaches to research. A challenge with performing a DNA microarray experiment in the teaching lab is determining the time required for the study and how to handle the voluminous data generated. At an unexpectedly low cost, a 6-week, project-based lab module has been developed that provides 3 weeks for wet lab (hands-on work with the DNA microarrays) and 3 weeks for dry lab (analyzing data, using databases to help with data analysis, and considering the meaning of data within the large dataset). Options exist for extending the number of weeks dedicated to the project, but 6 weeks is sufficient for providing an introduction to both experimental genomics and data analysis. Students indicate that being able to both perform array experiments and thoroughly analyze data enriches their understanding of genomics and the complexity of biological systems.
format	article
author	David B. Kushner
author_facet	David B. Kushner
author_sort	David B. Kushner
title	DNA Microarrays in the Undergraduate Microbiology Lab: Experimentation and Handling Large Datasets in as Few as Six Weeks
title_short	DNA Microarrays in the Undergraduate Microbiology Lab: Experimentation and Handling Large Datasets in as Few as Six Weeks
title_full	DNA Microarrays in the Undergraduate Microbiology Lab: Experimentation and Handling Large Datasets in as Few as Six Weeks
title_fullStr	DNA Microarrays in the Undergraduate Microbiology Lab: Experimentation and Handling Large Datasets in as Few as Six Weeks
title_full_unstemmed	DNA Microarrays in the Undergraduate Microbiology Lab: Experimentation and Handling Large Datasets in as Few as Six Weeks
title_sort	dna microarrays in the undergraduate microbiology lab: experimentation and handling large datasets in as few as six weeks
publisher	American Society for Microbiology
publishDate	2007
url	https://doaj.org/article/1cf8211273b3431c930de97a7bbb26db
work_keys_str_mv	AT davidbkushner dnamicroarraysintheundergraduatemicrobiologylabexperimentationandhandlinglargedatasetsinasfewassixweeks
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DNA Microarrays in the Undergraduate Microbiology Lab: Experimentation and Handling Large Datasets in as Few as Six Weeks

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