Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells

Gonadotropin-releasing hormone (GnRH), which is synthesized and released by the hypothalamus, promotes the synthesis and secretion of follicle-stimulating hormone (FSH), thereby regulating the growth and reproduction of animals. GnRH analogues have been widely used in livestock production. MiRNAs, w...

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Autores principales:	Hao-Qi Wang, Wen-Hua Wang, Cheng-Zhen Chen, Hai-Xiang Guo, Hao Jiang, Bao Yuan, Jia-Bao Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pituitary follicle-stimulating hormone gonadotropin-releasing hormone microRNA animal reproduction Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/1cf8e733498f4b9c9feea869db330312
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spelling	oai:doaj.org-article:1cf8e733498f4b9c9feea869db3303122021-11-25T16:19:58ZRegulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells10.3390/ani111132622076-2615https://doaj.org/article/1cf8e733498f4b9c9feea869db3303122021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3262https://doaj.org/toc/2076-2615Gonadotropin-releasing hormone (GnRH), which is synthesized and released by the hypothalamus, promotes the synthesis and secretion of follicle-stimulating hormone (FSH), thereby regulating the growth and reproduction of animals. GnRH analogues have been widely used in livestock production. MiRNAs, which are endogenous non-coding RNAs, have been found to play important roles in hormone regulation and other physiological processes in recent years. However, the roles of miRNAs in GnRH-mediated regulation of FSH secretion have rarely been studied. Herein, we treated bovine anterior adenohypophyseal cells with an exogenous GnRH analogue and found that miR-488 was differentially expressed. Through a combination of TargetScan prediction and dual luciferase reporter analysis, miR-488 was confirmed to be able to target the FSHB gene. Based on this finding, we verified the expression of Fshβ and Lhβ mRNA in the rat adenohypophysis before and after exogenous GnRH treatment in vivo and in vitro. Experiments on rat anterior adenohypophyseal cells showed that overexpression of miR-488 significantly inhibited Fshβ expression and FSH synthesis, while knockdown of miR-488 had the opposite effects. Our results demonstrate that GnRH relies on miR-488 to regulate FSH synthesis, providing additional useful evidence for the significance of miRNAs in the regulation of animal reproduction.Hao-Qi WangWen-Hua WangCheng-Zhen ChenHai-Xiang GuoHao JiangBao YuanJia-Bao ZhangMDPI AGarticlepituitaryfollicle-stimulating hormonegonadotropin-releasing hormonemicroRNAanimal reproductionVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3262, p 3262 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pituitary follicle-stimulating hormone gonadotropin-releasing hormone microRNA animal reproduction Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	pituitary follicle-stimulating hormone gonadotropin-releasing hormone microRNA animal reproduction Veterinary medicine SF600-1100 Zoology QL1-991 Hao-Qi Wang Wen-Hua Wang Cheng-Zhen Chen Hai-Xiang Guo Hao Jiang Bao Yuan Jia-Bao Zhang Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells
description	Gonadotropin-releasing hormone (GnRH), which is synthesized and released by the hypothalamus, promotes the synthesis and secretion of follicle-stimulating hormone (FSH), thereby regulating the growth and reproduction of animals. GnRH analogues have been widely used in livestock production. MiRNAs, which are endogenous non-coding RNAs, have been found to play important roles in hormone regulation and other physiological processes in recent years. However, the roles of miRNAs in GnRH-mediated regulation of FSH secretion have rarely been studied. Herein, we treated bovine anterior adenohypophyseal cells with an exogenous GnRH analogue and found that miR-488 was differentially expressed. Through a combination of TargetScan prediction and dual luciferase reporter analysis, miR-488 was confirmed to be able to target the FSHB gene. Based on this finding, we verified the expression of Fshβ and Lhβ mRNA in the rat adenohypophysis before and after exogenous GnRH treatment in vivo and in vitro. Experiments on rat anterior adenohypophyseal cells showed that overexpression of miR-488 significantly inhibited Fshβ expression and FSH synthesis, while knockdown of miR-488 had the opposite effects. Our results demonstrate that GnRH relies on miR-488 to regulate FSH synthesis, providing additional useful evidence for the significance of miRNAs in the regulation of animal reproduction.
format	article
author	Hao-Qi Wang Wen-Hua Wang Cheng-Zhen Chen Hai-Xiang Guo Hao Jiang Bao Yuan Jia-Bao Zhang
author_facet	Hao-Qi Wang Wen-Hua Wang Cheng-Zhen Chen Hai-Xiang Guo Hao Jiang Bao Yuan Jia-Bao Zhang
author_sort	Hao-Qi Wang
title	Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells
title_short	Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells
title_full	Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells
title_fullStr	Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells
title_full_unstemmed	Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells
title_sort	regulation of fsh synthesis by differentially expressed mir-488 in anterior adenohypophyseal cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1cf8e733498f4b9c9feea869db330312
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Regulation of FSH Synthesis by Differentially Expressed miR-488 in Anterior Adenohypophyseal Cells

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