Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model

Introduction: Retinal Pigment Epithelium (RPE) layer deterioration is a leading cause of Age-Related Macular Degeneration (AMD), i.e., the most significant reason for irreversible blindness. The present study aimed to track the Neurosphere-Derived (NS) from Bone Marrow Stromal Stem Cells (BMSCs) gra...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hamid Aboutaleb Kadkhodaeian, Amir Salati, Mojtaba Ansari, Vajihe Taghdiri Nooshabadi
Formato:	article
Lenguaje:	EN
Publicado:	Iran University of Medical Sciences 2021
Materias:	neurosphere cells bone marrow stromal stem cells sodium iodate age-related macular degeneration Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/1d02449a0dc84f62810e52fe5c7ba13c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1d02449a0dc84f62810e52fe5c7ba13c
record_format	dspace
spelling	oai:doaj.org-article:1d02449a0dc84f62810e52fe5c7ba13c2021-12-05T08:43:44ZTracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model2008-126X2228-7442https://doaj.org/article/1d02449a0dc84f62810e52fe5c7ba13c2021-07-01T00:00:00Zhttp://bcn.iums.ac.ir/article-1-1662-en.htmlhttps://doaj.org/toc/2008-126Xhttps://doaj.org/toc/2228-7442Introduction: Retinal Pigment Epithelium (RPE) layer deterioration is a leading cause of Age-Related Macular Degeneration (AMD), i.e., the most significant reason for irreversible blindness. The present study aimed to track the Neurosphere-Derived (NS) from Bone Marrow Stromal Stem Cells (BMSCs) grafted into the sub-retinal space (destruction of the RPE layer by sodium iodate). Methods: RPE degeneration model was performed using the injection of 5% sodium iodate performed in the retro-orbital sinus of Wistar rats. BMSCs were extracted from the examined rat femur and induced into NS, using EGF, bFGF, and B27. BrdU-NS labeled cells were transplanted into the sub-retinal space. For detecting BMSCs and NS markers, immunocytochemistry was performed. Moreover, immunohistochemical was conducted for tracking the transplanted cells in the RPE and sensory retina. Results: The immunocytochemistry of BMSCs cells displayed the expression of mesenchymal stem cells markers (CD90; 99%±1), CD166 (98%±2), CD44 (99%±1). Additionally, the expression of neural lineage markers in NS, such as SOX2, OCT4, Nanog, Nestin, and Neurofilaments (68, 160, 200) revealed the differentiation from BMSCs. Tracking BrdU-NS labeled suggested these aggregations in most layers of the retina. Conclusion: Our study data indicated that BMSCs derived neurosphere had the potential to migrate in injured retinal and integrate into the neurosensory retina. These data can be useful in finding safe cells for replacement therapy in AMD.Hamid Aboutaleb KadkhodaeianAmir SalatiMojtaba AnsariVajihe Taghdiri NooshabadiIran University of Medical Sciencesarticleneurosphere cellsbone marrow stromal stem cellssodium iodateage-related macular degenerationNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENBasic and Clinical Neuroscience, Vol 12, Iss 4, Pp 523-532 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neurosphere cells bone marrow stromal stem cells sodium iodate age-related macular degeneration Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	neurosphere cells bone marrow stromal stem cells sodium iodate age-related macular degeneration Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Hamid Aboutaleb Kadkhodaeian Amir Salati Mojtaba Ansari Vajihe Taghdiri Nooshabadi Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model
description	Introduction: Retinal Pigment Epithelium (RPE) layer deterioration is a leading cause of Age-Related Macular Degeneration (AMD), i.e., the most significant reason for irreversible blindness. The present study aimed to track the Neurosphere-Derived (NS) from Bone Marrow Stromal Stem Cells (BMSCs) grafted into the sub-retinal space (destruction of the RPE layer by sodium iodate). Methods: RPE degeneration model was performed using the injection of 5% sodium iodate performed in the retro-orbital sinus of Wistar rats. BMSCs were extracted from the examined rat femur and induced into NS, using EGF, bFGF, and B27. BrdU-NS labeled cells were transplanted into the sub-retinal space. For detecting BMSCs and NS markers, immunocytochemistry was performed. Moreover, immunohistochemical was conducted for tracking the transplanted cells in the RPE and sensory retina. Results: The immunocytochemistry of BMSCs cells displayed the expression of mesenchymal stem cells markers (CD90; 99%±1), CD166 (98%±2), CD44 (99%±1). Additionally, the expression of neural lineage markers in NS, such as SOX2, OCT4, Nanog, Nestin, and Neurofilaments (68, 160, 200) revealed the differentiation from BMSCs. Tracking BrdU-NS labeled suggested these aggregations in most layers of the retina. Conclusion: Our study data indicated that BMSCs derived neurosphere had the potential to migrate in injured retinal and integrate into the neurosensory retina. These data can be useful in finding safe cells for replacement therapy in AMD.
format	article
author	Hamid Aboutaleb Kadkhodaeian Amir Salati Mojtaba Ansari Vajihe Taghdiri Nooshabadi
author_facet	Hamid Aboutaleb Kadkhodaeian Amir Salati Mojtaba Ansari Vajihe Taghdiri Nooshabadi
author_sort	Hamid Aboutaleb Kadkhodaeian
title	Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model
title_short	Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model
title_full	Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model
title_fullStr	Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model
title_full_unstemmed	Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model
title_sort	tracking the transplanted neurosphere in retinal pigment epithelium degeneration model
publisher	Iran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/1d02449a0dc84f62810e52fe5c7ba13c
work_keys_str_mv	AT hamidaboutalebkadkhodaeian trackingthetransplantedneurosphereinretinalpigmentepitheliumdegenerationmodel AT amirsalati trackingthetransplantedneurosphereinretinalpigmentepitheliumdegenerationmodel AT mojtabaansari trackingthetransplantedneurosphereinretinalpigmentepitheliumdegenerationmodel AT vajihetaghdirinooshabadi trackingthetransplantedneurosphereinretinalpigmentepitheliumdegenerationmodel
_version_	1718372542524162048

Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model

Ejemplares similares