MAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRY

Abstract. Determination of lymphocyte subpopulations and their phenotypes is an important diagnostic feature, in order to elucidate some disturbances connected with immune system functioning. However, insufficient data are obtained when analyzing only major populations of peripheral lymphocytes. In...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	S. V. Khaidukov, A. V. Zurochka, Areg A. Totolian, V. A. Chereshnev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	flow cytometry immunophenotyping т-lymphocytes в-lymphocytes nk-cells Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1d03f77e979f40609d0327e397dd8859
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1d03f77e979f40609d0327e397dd8859
record_format	dspace
spelling	oai:doaj.org-article:1d03f77e979f40609d0327e397dd88592021-11-18T08:03:40ZMAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRY1563-06252313-741X10.15789/1563-0625-2009-2-3-227-238https://doaj.org/article/1d03f77e979f40609d0327e397dd88592014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/308https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Determination of lymphocyte subpopulations and their phenotypes is an important diagnostic feature, in order to elucidate some disturbances connected with immune system functioning. However, insufficient data are obtained when analyzing only major populations of peripheral lymphocytes. In order to perform clinical diagnostics, the data about minor lymphocytic populations and activated cellular pools seem to be more pertinent.Studies of peripheral blood cell subpopulations of healthy donors performed in different Russian regions allowed to assess quantitative distribution intervals for both major and minor immune cell subpopulations in humans. The results obtained, as compared with data from literature, provide an evidence for similar reference intervals for main immune cell subpopulations in healthy donors, independent on their habitation area.Present work has resulted into development of algorithms for cytometric studies and generation of certain panels of monoclonal antibodies enabling evaluation of all main lymphocyte subpopulations, as well as their minor subsets participating in emerging immune response. The distribution intervals have been estimated for such minor subpopulations, as B1- and B2-lymphocytes, memory B-cells, γδ- and αβT-cells, regulatory and naїve T-cells, cytotoxic and secretory NK-cell polupations.The results of present study, while been performed with peripheral blood of healthy donors, may provide a basis of reference values when studying subpopulation profile of immune cells.S. V. KhaidukovA. V. ZurochkaAreg A. TotolianV. A. ChereshnevSPb RAACIarticleflow cytometryimmunophenotypingт-lymphocytesв-lymphocytesnk-cellsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 11, Iss 2-3, Pp 227-238 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	flow cytometry immunophenotyping т-lymphocytes в-lymphocytes nk-cells Immunologic diseases. Allergy RC581-607
spellingShingle	flow cytometry immunophenotyping т-lymphocytes в-lymphocytes nk-cells Immunologic diseases. Allergy RC581-607 S. V. Khaidukov A. V. Zurochka Areg A. Totolian V. A. Chereshnev MAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRY
description	Abstract. Determination of lymphocyte subpopulations and their phenotypes is an important diagnostic feature, in order to elucidate some disturbances connected with immune system functioning. However, insufficient data are obtained when analyzing only major populations of peripheral lymphocytes. In order to perform clinical diagnostics, the data about minor lymphocytic populations and activated cellular pools seem to be more pertinent.Studies of peripheral blood cell subpopulations of healthy donors performed in different Russian regions allowed to assess quantitative distribution intervals for both major and minor immune cell subpopulations in humans. The results obtained, as compared with data from literature, provide an evidence for similar reference intervals for main immune cell subpopulations in healthy donors, independent on their habitation area.Present work has resulted into development of algorithms for cytometric studies and generation of certain panels of monoclonal antibodies enabling evaluation of all main lymphocyte subpopulations, as well as their minor subsets participating in emerging immune response. The distribution intervals have been estimated for such minor subpopulations, as B1- and B2-lymphocytes, memory B-cells, γδ- and αβT-cells, regulatory and naїve T-cells, cytotoxic and secretory NK-cell polupations.The results of present study, while been performed with peripheral blood of healthy donors, may provide a basis of reference values when studying subpopulation profile of immune cells.
format	article
author	S. V. Khaidukov A. V. Zurochka Areg A. Totolian V. A. Chereshnev
author_facet	S. V. Khaidukov A. V. Zurochka Areg A. Totolian V. A. Chereshnev
author_sort	S. V. Khaidukov
title	MAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRY
title_short	MAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRY
title_full	MAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRY
title_fullStr	MAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRY
title_full_unstemmed	MAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRY
title_sort	major and lymphocyte populations of human peripheral blood lymphocytes and their reference values, as assayed by multi-colour cytometry
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/1d03f77e979f40609d0327e397dd8859
work_keys_str_mv	AT svkhaidukov majorandlymphocytepopulationsofhumanperipheralbloodlymphocytesandtheirreferencevaluesasassayedbymulticolourcytometry AT avzurochka majorandlymphocytepopulationsofhumanperipheralbloodlymphocytesandtheirreferencevaluesasassayedbymulticolourcytometry AT aregatotolian majorandlymphocytepopulationsofhumanperipheralbloodlymphocytesandtheirreferencevaluesasassayedbymulticolourcytometry AT vachereshnev majorandlymphocytepopulationsofhumanperipheralbloodlymphocytesandtheirreferencevaluesasassayedbymulticolourcytometry
_version_	1718422529057488896

MAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRY

Ejemplares similares