Biochemical pathways and enhanced degradation of dioctyl phthalate (DEHP) by sodium alginate immobilization in MBR system

As one of the most representative endocrine disrupting compounds, dioctyl phthalate (DEHP) is difficult to remove due to its bio-refractory characteristic. In this study, an immobilization technology was applied in an MBR system to improve the degradation of DEHP. The degradation efficiency of DEHP...

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Autores principales:	Ke Zhang, Xiangling Wu, Hongbing Luo, Wei Wang, Siqiao Yang, Jian Chen, Wei Chen, Jia Chen, You Mo, Lin Li
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	3 4-dioxygenase genes bacterial community dynamies biodegradation pathway enhanced biodegradation immobilized dehp degrading bacteria mbr system Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/1d0407d84f47455290cdc8478ed0b825
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spelling	oai:doaj.org-article:1d0407d84f47455290cdc8478ed0b8252021-11-06T10:42:37ZBiochemical pathways and enhanced degradation of dioctyl phthalate (DEHP) by sodium alginate immobilization in MBR system0273-12231996-973210.2166/wst.2020.605https://doaj.org/article/1d0407d84f47455290cdc8478ed0b8252021-02-01T00:00:00Zhttp://wst.iwaponline.com/content/83/3/664https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732As one of the most representative endocrine disrupting compounds, dioctyl phthalate (DEHP) is difficult to remove due to its bio-refractory characteristic. In this study, an immobilization technology was applied in an MBR system to improve the degradation of DEHP. The degradation efficiency of DEHP was significantly improved and the number of degradation genes increased by 1/3. A bacterial strain that could effectively degrade DEHP was isolated from activated sludge and identified as Bacillus sp. The degradation pathway of DEHP was analyzed by GC-MS. DEHP was decomposed into phthalates (DBP) and Diuretic sylycol (DEP), then further to Phthalic acid (PA). PA was oxidized, dehydrogenated, and decarboxylated into protocatechins, further entered the TCA cycle through orthotopic ring opening. The DEHP degrading strain was immobilized by sodium alginate and calcium chloride under the optimized immobilization conditions, and added to MBR systems. The removal rate of DEHP (5 mg/L) (91.9%) and the number of 3, 4-dioxygenase gene copies was significantly improved by adding immobilized bacteria. Micromonospora, Rhodococcus, Bacteroides and Pseudomonas were the dominant genuses, and the results of bacterial community structure analysis show that immobilization technology is beneficial to system stability. The results showed the potential applications of the immobilized technique in DEHP wastewater treatment in MBR.Ke ZhangXiangling WuHongbing LuoWei WangSiqiao YangJian ChenWei ChenJia ChenYou MoLin LiIWA Publishingarticle34-dioxygenase genesbacterial community dynamiesbiodegradation pathwayenhanced biodegradationimmobilized dehp degrading bacteriambr systemEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 3, Pp 664-677 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	3 4-dioxygenase genes bacterial community dynamies biodegradation pathway enhanced biodegradation immobilized dehp degrading bacteria mbr system Environmental technology. Sanitary engineering TD1-1066
spellingShingle	3 4-dioxygenase genes bacterial community dynamies biodegradation pathway enhanced biodegradation immobilized dehp degrading bacteria mbr system Environmental technology. Sanitary engineering TD1-1066 Ke Zhang Xiangling Wu Hongbing Luo Wei Wang Siqiao Yang Jian Chen Wei Chen Jia Chen You Mo Lin Li Biochemical pathways and enhanced degradation of dioctyl phthalate (DEHP) by sodium alginate immobilization in MBR system
description	As one of the most representative endocrine disrupting compounds, dioctyl phthalate (DEHP) is difficult to remove due to its bio-refractory characteristic. In this study, an immobilization technology was applied in an MBR system to improve the degradation of DEHP. The degradation efficiency of DEHP was significantly improved and the number of degradation genes increased by 1/3. A bacterial strain that could effectively degrade DEHP was isolated from activated sludge and identified as Bacillus sp. The degradation pathway of DEHP was analyzed by GC-MS. DEHP was decomposed into phthalates (DBP) and Diuretic sylycol (DEP), then further to Phthalic acid (PA). PA was oxidized, dehydrogenated, and decarboxylated into protocatechins, further entered the TCA cycle through orthotopic ring opening. The DEHP degrading strain was immobilized by sodium alginate and calcium chloride under the optimized immobilization conditions, and added to MBR systems. The removal rate of DEHP (5 mg/L) (91.9%) and the number of 3, 4-dioxygenase gene copies was significantly improved by adding immobilized bacteria. Micromonospora, Rhodococcus, Bacteroides and Pseudomonas were the dominant genuses, and the results of bacterial community structure analysis show that immobilization technology is beneficial to system stability. The results showed the potential applications of the immobilized technique in DEHP wastewater treatment in MBR.
format	article
author	Ke Zhang Xiangling Wu Hongbing Luo Wei Wang Siqiao Yang Jian Chen Wei Chen Jia Chen You Mo Lin Li
author_facet	Ke Zhang Xiangling Wu Hongbing Luo Wei Wang Siqiao Yang Jian Chen Wei Chen Jia Chen You Mo Lin Li
author_sort	Ke Zhang
title	Biochemical pathways and enhanced degradation of dioctyl phthalate (DEHP) by sodium alginate immobilization in MBR system
title_short	Biochemical pathways and enhanced degradation of dioctyl phthalate (DEHP) by sodium alginate immobilization in MBR system
title_full	Biochemical pathways and enhanced degradation of dioctyl phthalate (DEHP) by sodium alginate immobilization in MBR system
title_fullStr	Biochemical pathways and enhanced degradation of dioctyl phthalate (DEHP) by sodium alginate immobilization in MBR system
title_full_unstemmed	Biochemical pathways and enhanced degradation of dioctyl phthalate (DEHP) by sodium alginate immobilization in MBR system
title_sort	biochemical pathways and enhanced degradation of dioctyl phthalate (dehp) by sodium alginate immobilization in mbr system
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/1d0407d84f47455290cdc8478ed0b825
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Biochemical pathways and enhanced degradation of dioctyl phthalate (DEHP) by sodium alginate immobilization in MBR system

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