ApoE production in human monocytes and its regulation by inflammatory cytokines.

The apoE production by tissue macrophages is crucial for the prevention of atherosclerosis and the aim of this study was to further elucidate how this apolipoprotein is regulated by cytokines present during inflammation. Here we studied apoE production in peripheral blood mononuclear cells (PBMC) an...

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Autores principales:	Sten Braesch-Andersen, Staffan Paulie, Christian Smedman, Sohel Mia, Makiko Kumagai-Braesch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d0cbc205be446d682aa601ea12b1c95
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spelling	oai:doaj.org-article:1d0cbc205be446d682aa601ea12b1c952021-11-18T08:46:29ZApoE production in human monocytes and its regulation by inflammatory cytokines.1932-620310.1371/journal.pone.0079908https://doaj.org/article/1d0cbc205be446d682aa601ea12b1c952013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24244577/?tool=EBIhttps://doaj.org/toc/1932-6203The apoE production by tissue macrophages is crucial for the prevention of atherosclerosis and the aim of this study was to further elucidate how this apolipoprotein is regulated by cytokines present during inflammation. Here we studied apoE production in peripheral blood mononuclear cells (PBMC) and analysis was made with a newly developed apoE ELISpot assay. In PBMC, apoE secretion was restricted to monocytes with classical (CD14(++)CD16(-)) and intermediate (CD14(+)CD16(+)) monocytes being the main producers. As earlier described for macrophages, production was strongly upregulated by TGF-β and downregulated by bacterial lipopolysaccharide (LPS) and the inflammatory cytokines IFN-γ, TNF-α and IL-1β. We could here show that a similar down-regulatory effect was also observed with the type I interferon, IFN-α, while IL-6, often regarded as one of the more prominent inflammatory cytokines, did not affect TGF-β-induced apoE production. The TNF-α inhibitor Enbrel could partly block the down-regulatory effect of IFN-γ, IFN-α and IL-1β, indicating that inhibition of apoE by these cytokines may be dependent on or synergize with TNF-α. Other cytokines tested, IL-2, IL-4, IL-12, IL-13, IL-17A and IL-23, had no inhibitory effect on apoE production. In contrast to the effect on monocytes, apoE production by primary hepatocytes and the hepatoma cell line HepG2 was more or less unaffected by treatment with cytokines or LPS.Sten Braesch-AndersenStaffan PaulieChristian SmedmanSohel MiaMakiko Kumagai-BraeschPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79908 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sten Braesch-Andersen Staffan Paulie Christian Smedman Sohel Mia Makiko Kumagai-Braesch ApoE production in human monocytes and its regulation by inflammatory cytokines.
description	The apoE production by tissue macrophages is crucial for the prevention of atherosclerosis and the aim of this study was to further elucidate how this apolipoprotein is regulated by cytokines present during inflammation. Here we studied apoE production in peripheral blood mononuclear cells (PBMC) and analysis was made with a newly developed apoE ELISpot assay. In PBMC, apoE secretion was restricted to monocytes with classical (CD14(++)CD16(-)) and intermediate (CD14(+)CD16(+)) monocytes being the main producers. As earlier described for macrophages, production was strongly upregulated by TGF-β and downregulated by bacterial lipopolysaccharide (LPS) and the inflammatory cytokines IFN-γ, TNF-α and IL-1β. We could here show that a similar down-regulatory effect was also observed with the type I interferon, IFN-α, while IL-6, often regarded as one of the more prominent inflammatory cytokines, did not affect TGF-β-induced apoE production. The TNF-α inhibitor Enbrel could partly block the down-regulatory effect of IFN-γ, IFN-α and IL-1β, indicating that inhibition of apoE by these cytokines may be dependent on or synergize with TNF-α. Other cytokines tested, IL-2, IL-4, IL-12, IL-13, IL-17A and IL-23, had no inhibitory effect on apoE production. In contrast to the effect on monocytes, apoE production by primary hepatocytes and the hepatoma cell line HepG2 was more or less unaffected by treatment with cytokines or LPS.
format	article
author	Sten Braesch-Andersen Staffan Paulie Christian Smedman Sohel Mia Makiko Kumagai-Braesch
author_facet	Sten Braesch-Andersen Staffan Paulie Christian Smedman Sohel Mia Makiko Kumagai-Braesch
author_sort	Sten Braesch-Andersen
title	ApoE production in human monocytes and its regulation by inflammatory cytokines.
title_short	ApoE production in human monocytes and its regulation by inflammatory cytokines.
title_full	ApoE production in human monocytes and its regulation by inflammatory cytokines.
title_fullStr	ApoE production in human monocytes and its regulation by inflammatory cytokines.
title_full_unstemmed	ApoE production in human monocytes and its regulation by inflammatory cytokines.
title_sort	apoe production in human monocytes and its regulation by inflammatory cytokines.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1d0cbc205be446d682aa601ea12b1c95
work_keys_str_mv	AT stenbraeschandersen apoeproductioninhumanmonocytesanditsregulationbyinflammatorycytokines AT staffanpaulie apoeproductioninhumanmonocytesanditsregulationbyinflammatorycytokines AT christiansmedman apoeproductioninhumanmonocytesanditsregulationbyinflammatorycytokines AT sohelmia apoeproductioninhumanmonocytesanditsregulationbyinflammatorycytokines AT makikokumagaibraesch apoeproductioninhumanmonocytesanditsregulationbyinflammatorycytokines
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ApoE production in human monocytes and its regulation by inflammatory cytokines.

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