Optimization of single-walled carbon nanotube solubility by noncovalent PEGylation using experimental design methods

Naghmeh Hadidi1, Farzad Kobarfard2, Nastaran Nafissi-Varcheh3, Reza Aboofazeli11Department of Pharmaceutics, 2Department of Pharmaceutical Chemistry, 3Department of Pharmaceutical Biotechnology, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, IranAbstract: In this study,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hadidi N, Kobarfard F, Nafissi-Varcheh N, Aboofazeli R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1d12111eeac448309f84fe502e3c63f5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1d12111eeac448309f84fe502e3c63f5
record_format	dspace
spelling	oai:doaj.org-article:1d12111eeac448309f84fe502e3c63f52021-12-02T04:46:23ZOptimization of single-walled carbon nanotube solubility by noncovalent PEGylation using experimental design methods1176-91141178-2013https://doaj.org/article/1d12111eeac448309f84fe502e3c63f52011-04-01T00:00:00Zhttp://www.dovepress.com/optimization-of-single-walled-carbon-nanotube-solubility-by-noncovalen-a7049https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Naghmeh Hadidi1, Farzad Kobarfard2, Nastaran Nafissi-Varcheh3, Reza Aboofazeli11Department of Pharmaceutics, 2Department of Pharmaceutical Chemistry, 3Department of Pharmaceutical Biotechnology, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, IranAbstract: In this study, noncovalent functionalization of single-walled carbon nanotubes (SWCNTs) with phospholipid-polyethylene glycols (Pl-PEGs) was performed to improve the solubility of SWCNTs in aqueous solution. Two kinds of PEG derivatives, ie, Pl-PEG 2000 and Pl-PEG 5000, were used for the PEGylation process. An experimental design technique (D-optimal design and second-order polynomial equations) was applied to investigate the effect of variables on PEGylation and the solubility of SWCNTs. The type of PEG derivative was selected as a qualitative parameter, and the PEG/SWCNT weight ratio and sonication time were applied as quantitative variables for the experimental design. Optimization was performed for two responses, aqueous solubility and loading efficiency. The grafting of PEG to the carbon nanostructure was determined by thermogravimetric analysis, Raman spectroscopy, and scanning electron microscopy. Aqueous solubility and loading efficiency were determined by ultraviolet-visible spectrophotometry and measurement of free amine groups, respectively. Results showed that Pl-PEGs were grafted onto SWCNTs. Aqueous solubility of 0.84 mg/mL and loading efficiency of nearly 98% were achieved for the prepared Pl-PEG 5000-SWCNT conjugates. Evaluation of functionalized SWCNTs showed that our noncovalent functionalization protocol could considerably increase aqueous solubility, which is an essential criterion in the design of a carbon nanotube-based drug delivery system and its biodistribution.Keywords: phospholipid-PEG, D-optimal design, loading efficiency, Raman spectroscopy, scanning electron microscopy, theromogravimetric analysis, carbon nanotubes Hadidi NKobarfard FNafissi-Varcheh NAboofazeli RDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 737-746 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Hadidi N Kobarfard F Nafissi-Varcheh N Aboofazeli R Optimization of single-walled carbon nanotube solubility by noncovalent PEGylation using experimental design methods
description	Naghmeh Hadidi1, Farzad Kobarfard2, Nastaran Nafissi-Varcheh3, Reza Aboofazeli11Department of Pharmaceutics, 2Department of Pharmaceutical Chemistry, 3Department of Pharmaceutical Biotechnology, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, IranAbstract: In this study, noncovalent functionalization of single-walled carbon nanotubes (SWCNTs) with phospholipid-polyethylene glycols (Pl-PEGs) was performed to improve the solubility of SWCNTs in aqueous solution. Two kinds of PEG derivatives, ie, Pl-PEG 2000 and Pl-PEG 5000, were used for the PEGylation process. An experimental design technique (D-optimal design and second-order polynomial equations) was applied to investigate the effect of variables on PEGylation and the solubility of SWCNTs. The type of PEG derivative was selected as a qualitative parameter, and the PEG/SWCNT weight ratio and sonication time were applied as quantitative variables for the experimental design. Optimization was performed for two responses, aqueous solubility and loading efficiency. The grafting of PEG to the carbon nanostructure was determined by thermogravimetric analysis, Raman spectroscopy, and scanning electron microscopy. Aqueous solubility and loading efficiency were determined by ultraviolet-visible spectrophotometry and measurement of free amine groups, respectively. Results showed that Pl-PEGs were grafted onto SWCNTs. Aqueous solubility of 0.84 mg/mL and loading efficiency of nearly 98% were achieved for the prepared Pl-PEG 5000-SWCNT conjugates. Evaluation of functionalized SWCNTs showed that our noncovalent functionalization protocol could considerably increase aqueous solubility, which is an essential criterion in the design of a carbon nanotube-based drug delivery system and its biodistribution.Keywords: phospholipid-PEG, D-optimal design, loading efficiency, Raman spectroscopy, scanning electron microscopy, theromogravimetric analysis, carbon nanotubes
format	article
author	Hadidi N Kobarfard F Nafissi-Varcheh N Aboofazeli R
author_facet	Hadidi N Kobarfard F Nafissi-Varcheh N Aboofazeli R
author_sort	Hadidi N
title	Optimization of single-walled carbon nanotube solubility by noncovalent PEGylation using experimental design methods
title_short	Optimization of single-walled carbon nanotube solubility by noncovalent PEGylation using experimental design methods
title_full	Optimization of single-walled carbon nanotube solubility by noncovalent PEGylation using experimental design methods
title_fullStr	Optimization of single-walled carbon nanotube solubility by noncovalent PEGylation using experimental design methods
title_full_unstemmed	Optimization of single-walled carbon nanotube solubility by noncovalent PEGylation using experimental design methods
title_sort	optimization of single-walled carbon nanotube solubility by noncovalent pegylation using experimental design methods
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/1d12111eeac448309f84fe502e3c63f5
work_keys_str_mv	AT hadidin optimizationofsinglewalledcarbonnanotubesolubilitybynoncovalentpegylationusingexperimentaldesignmethods AT kobarfardf optimizationofsinglewalledcarbonnanotubesolubilitybynoncovalentpegylationusingexperimentaldesignmethods AT nafissivarchehn optimizationofsinglewalledcarbonnanotubesolubilitybynoncovalentpegylationusingexperimentaldesignmethods AT aboofazelir optimizationofsinglewalledcarbonnanotubesolubilitybynoncovalentpegylationusingexperimentaldesignmethods
_version_	1718401050752319488

Optimization of single-walled carbon nanotube solubility by noncovalent PEGylation using experimental design methods

Ejemplares similares