Amyloid-like staining property of RADA16-I nanofibers and its potential application in detecting and imaging the nanomaterial

Yongzhu Chen,1 Yusi Hua,2 Wensheng Zhang,3 Chengkang Tang,4 Yan Wang,4 Yujun Zhang,3 Feng Qiu3 1Periodical Press of West China Hospital, Sichuan University, Chengdu, China; 2Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China; 3Laboratory of Anaesthesia and Critica...

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Autores principales:	Chen Y, Hua Y, Zhang W, Tang C, Wang Y, Zhang Y, Qiu F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	self-assembling peptides nanofibers amyloid fibrils thioflavin T Congo red Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1d1db5d554b34c0ea8b05bad6246d8ee
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spelling	oai:doaj.org-article:1d1db5d554b34c0ea8b05bad6246d8ee2021-12-02T04:22:37ZAmyloid-like staining property of RADA16-I nanofibers and its potential application in detecting and imaging the nanomaterial1178-2013https://doaj.org/article/1d1db5d554b34c0ea8b05bad6246d8ee2018-04-01T00:00:00Zhttps://www.dovepress.com/amyloid-like-staining-property-of-rada16-i-nanofibers-and-its-potentia-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yongzhu Chen,1 Yusi Hua,2 Wensheng Zhang,3 Chengkang Tang,4 Yan Wang,4 Yujun Zhang,3 Feng Qiu3 1Periodical Press of West China Hospital, Sichuan University, Chengdu, China; 2Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China; 3Laboratory of Anaesthesia and Critical Care Medicine, Translational Neuroscience Centre, West China Hospital, Sichuan University, Chengdu, China; 4Core Facility of West China Hospital, Sichuan University, Chengdu, China Background: Designer self-assembling peptide nanofibers (SAPNFs) as a novel kind of emerging nanomaterial have received more and more attention in the field of nanomedicine in recent years. However, a simple method to monitor and image SAPNFs is still currently absent. Methods: RADA16-I, a well-studied ionic complementary peptide was used as a model to check potential amyloid-like staining properties of SAPNFs. Thioflavin-T (ThT) and Congo red (CR) as specific dyes for amyloid-like fibrils were used to stain RADA16-I nanofibers in solution, combined with drugs or cells, or injected in vivo as hydrogels. Fluorescent spectrometry and fluorescent microscopy were used to check ThT-binding property, and polarized light microscopy was used to check CR-staining property. Results: ThT binding with the nanofibers showed enhanced and blue-shifted fluorescence, and specific apple-green birefringence could be observed after the nanofibers were stained with CR. Based on these properties we further showed that ThT-binding fluorescence intensity could be used to monitor the forming and changing of nanofibers in solution, while fluorescent microscopy and polarized light microscopy could be used to image the nanofibers as material for drug delivery, 3D cell culture, and tissue regeneration. Conclusion: Our results may provide convenient and reliable tools for detecting SAPNFs, which would be helpful for understanding their self-assembling process and exploring their applications. Keywords: self-assembling peptides, nanofibers, amyloid fibrils, thioflavin-T, Congo redChen YHua YZhang WTang CWang YZhang YQiu FDove Medical Pressarticleself-assembling peptidesnanofibersamyloid fibrilsthioflavin TCongo redMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 2477-2489 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	self-assembling peptides nanofibers amyloid fibrils thioflavin T Congo red Medicine (General) R5-920
spellingShingle	self-assembling peptides nanofibers amyloid fibrils thioflavin T Congo red Medicine (General) R5-920 Chen Y Hua Y Zhang W Tang C Wang Y Zhang Y Qiu F Amyloid-like staining property of RADA16-I nanofibers and its potential application in detecting and imaging the nanomaterial
description	Yongzhu Chen,1 Yusi Hua,2 Wensheng Zhang,3 Chengkang Tang,4 Yan Wang,4 Yujun Zhang,3 Feng Qiu3 1Periodical Press of West China Hospital, Sichuan University, Chengdu, China; 2Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China; 3Laboratory of Anaesthesia and Critical Care Medicine, Translational Neuroscience Centre, West China Hospital, Sichuan University, Chengdu, China; 4Core Facility of West China Hospital, Sichuan University, Chengdu, China Background: Designer self-assembling peptide nanofibers (SAPNFs) as a novel kind of emerging nanomaterial have received more and more attention in the field of nanomedicine in recent years. However, a simple method to monitor and image SAPNFs is still currently absent. Methods: RADA16-I, a well-studied ionic complementary peptide was used as a model to check potential amyloid-like staining properties of SAPNFs. Thioflavin-T (ThT) and Congo red (CR) as specific dyes for amyloid-like fibrils were used to stain RADA16-I nanofibers in solution, combined with drugs or cells, or injected in vivo as hydrogels. Fluorescent spectrometry and fluorescent microscopy were used to check ThT-binding property, and polarized light microscopy was used to check CR-staining property. Results: ThT binding with the nanofibers showed enhanced and blue-shifted fluorescence, and specific apple-green birefringence could be observed after the nanofibers were stained with CR. Based on these properties we further showed that ThT-binding fluorescence intensity could be used to monitor the forming and changing of nanofibers in solution, while fluorescent microscopy and polarized light microscopy could be used to image the nanofibers as material for drug delivery, 3D cell culture, and tissue regeneration. Conclusion: Our results may provide convenient and reliable tools for detecting SAPNFs, which would be helpful for understanding their self-assembling process and exploring their applications. Keywords: self-assembling peptides, nanofibers, amyloid fibrils, thioflavin-T, Congo red
format	article
author	Chen Y Hua Y Zhang W Tang C Wang Y Zhang Y Qiu F
author_facet	Chen Y Hua Y Zhang W Tang C Wang Y Zhang Y Qiu F
author_sort	Chen Y
title	Amyloid-like staining property of RADA16-I nanofibers and its potential application in detecting and imaging the nanomaterial
title_short	Amyloid-like staining property of RADA16-I nanofibers and its potential application in detecting and imaging the nanomaterial
title_full	Amyloid-like staining property of RADA16-I nanofibers and its potential application in detecting and imaging the nanomaterial
title_fullStr	Amyloid-like staining property of RADA16-I nanofibers and its potential application in detecting and imaging the nanomaterial
title_full_unstemmed	Amyloid-like staining property of RADA16-I nanofibers and its potential application in detecting and imaging the nanomaterial
title_sort	amyloid-like staining property of rada16-i nanofibers and its potential application in detecting and imaging the nanomaterial
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/1d1db5d554b34c0ea8b05bad6246d8ee
work_keys_str_mv	AT cheny amyloidlikestainingpropertyofrada16inanofibersanditspotentialapplicationindetectingandimagingthenanomaterial AT huay amyloidlikestainingpropertyofrada16inanofibersanditspotentialapplicationindetectingandimagingthenanomaterial AT zhangw amyloidlikestainingpropertyofrada16inanofibersanditspotentialapplicationindetectingandimagingthenanomaterial AT tangc amyloidlikestainingpropertyofrada16inanofibersanditspotentialapplicationindetectingandimagingthenanomaterial AT wangy amyloidlikestainingpropertyofrada16inanofibersanditspotentialapplicationindetectingandimagingthenanomaterial AT zhangy amyloidlikestainingpropertyofrada16inanofibersanditspotentialapplicationindetectingandimagingthenanomaterial AT qiuf amyloidlikestainingpropertyofrada16inanofibersanditspotentialapplicationindetectingandimagingthenanomaterial
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Amyloid-like staining property of RADA16-I nanofibers and its potential application in detecting and imaging the nanomaterial

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