Characterization of Four Novel dsRNA Viruses Isolated from Mucor hiemalis Strains

We previously screened the total nucleic acid extracts of 123 Mucor strains for the presence of dsRNA molecules without further molecular analyses. Here, we characterized five novel dsRNA genomes isolated from four different Mucor hiemalis str...

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Autores principales:	Tünde Kartali, Ildikó Nyilasi, Sándor Kocsubé, Roland Patai, Tamás F. Polgár, Nóra Zsindely, Gábor Nagy, László Bodai, Zoltán Lipinszki, Csaba Vágvölgyi, Tamás Papp
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mycovirus dsRNA virus particle <i>Totivirus</i> <i>Victorivirus</i> Mucorales Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1d2244daf5f74c28ae08595010a89af5
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spelling	oai:doaj.org-article:1d2244daf5f74c28ae08595010a89af52021-11-25T19:14:31ZCharacterization of Four Novel dsRNA Viruses Isolated from <i>Mucor</i> <i>hiemalis</i> Strains10.3390/v131123191999-4915https://doaj.org/article/1d2244daf5f74c28ae08595010a89af52021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2319https://doaj.org/toc/1999-4915We previously screened the total nucleic acid extracts of 123 <i>Mucor</i> strains for the presence of dsRNA molecules without further molecular analyses. Here, we characterized five novel dsRNA genomes isolated from four different <i>Mucor</i> <i>hiemalis</i> strains with next-generation sequencing (NGS), namely Mucor hiemalis virus 1a (MhV1a) from WRL CN(M) 122; Mucor hiemalis virus 1b (MhV1b) from NRRL 3624; Mucor hiemalis virus 2 (MhV2) from NRRL 3616; and Mucor hiemalis virus 3 (MhV3) and Mucor hiemalis virus (MhV4) from NRRL 3617 strains. Genomes contain two open reading frames (ORF), which encode the coat protein (CP) and the RNA dependent RNA polymerase (RdRp), respectively. In MhV1a and MhV1b, it is predicted to be translated as a fusion protein via -1 ribosomal frameshift, while in MhV4 via a rare +1 (or−2) ribosomal frameshift. In MhV2 and MhV3, the presence of specific UA<b>A</b><b>UG</b> pentanucleotide motif points to the fact for coupled translation termination and reinitialization. MhV1a, MhV2, and MhV3 are part of the clade representing the genus <i>Victorivirus</i>, while MhV4 is seated in <i>Totivirus</i> genus clade. The detected VLPs in <i>Mucor</i> strains were from 33 to 36 nm in diameter. Hybridization analysis revealed that the dsRNA molecules of MhV1a-MhV4 hybridized to the corresponding molecules.Tünde KartaliIldikó NyilasiSándor KocsubéRoland PataiTamás F. PolgárNóra ZsindelyGábor NagyLászló BodaiZoltán LipinszkiCsaba VágvölgyiTamás PappMDPI AGarticlemycovirusdsRNAvirus particle<i>Totivirus</i><i>Victorivirus</i>MucoralesMicrobiologyQR1-502ENViruses, Vol 13, Iss 2319, p 2319 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycovirus dsRNA virus particle <i>Totivirus</i> <i>Victorivirus</i> Mucorales Microbiology QR1-502
spellingShingle	mycovirus dsRNA virus particle <i>Totivirus</i> <i>Victorivirus</i> Mucorales Microbiology QR1-502 Tünde Kartali Ildikó Nyilasi Sándor Kocsubé Roland Patai Tamás F. Polgár Nóra Zsindely Gábor Nagy László Bodai Zoltán Lipinszki Csaba Vágvölgyi Tamás Papp Characterization of Four Novel dsRNA Viruses Isolated from <i>Mucor</i> <i>hiemalis</i> Strains
description	We previously screened the total nucleic acid extracts of 123 <i>Mucor</i> strains for the presence of dsRNA molecules without further molecular analyses. Here, we characterized five novel dsRNA genomes isolated from four different <i>Mucor</i> <i>hiemalis</i> strains with next-generation sequencing (NGS), namely Mucor hiemalis virus 1a (MhV1a) from WRL CN(M) 122; Mucor hiemalis virus 1b (MhV1b) from NRRL 3624; Mucor hiemalis virus 2 (MhV2) from NRRL 3616; and Mucor hiemalis virus 3 (MhV3) and Mucor hiemalis virus (MhV4) from NRRL 3617 strains. Genomes contain two open reading frames (ORF), which encode the coat protein (CP) and the RNA dependent RNA polymerase (RdRp), respectively. In MhV1a and MhV1b, it is predicted to be translated as a fusion protein via -1 ribosomal frameshift, while in MhV4 via a rare +1 (or−2) ribosomal frameshift. In MhV2 and MhV3, the presence of specific UA<b>A</b><b>UG</b> pentanucleotide motif points to the fact for coupled translation termination and reinitialization. MhV1a, MhV2, and MhV3 are part of the clade representing the genus <i>Victorivirus</i>, while MhV4 is seated in <i>Totivirus</i> genus clade. The detected VLPs in <i>Mucor</i> strains were from 33 to 36 nm in diameter. Hybridization analysis revealed that the dsRNA molecules of MhV1a-MhV4 hybridized to the corresponding molecules.
format	article
author	Tünde Kartali Ildikó Nyilasi Sándor Kocsubé Roland Patai Tamás F. Polgár Nóra Zsindely Gábor Nagy László Bodai Zoltán Lipinszki Csaba Vágvölgyi Tamás Papp
author_facet	Tünde Kartali Ildikó Nyilasi Sándor Kocsubé Roland Patai Tamás F. Polgár Nóra Zsindely Gábor Nagy László Bodai Zoltán Lipinszki Csaba Vágvölgyi Tamás Papp
author_sort	Tünde Kartali
title	Characterization of Four Novel dsRNA Viruses Isolated from <i>Mucor</i> <i>hiemalis</i> Strains
title_short	Characterization of Four Novel dsRNA Viruses Isolated from <i>Mucor</i> <i>hiemalis</i> Strains
title_full	Characterization of Four Novel dsRNA Viruses Isolated from <i>Mucor</i> <i>hiemalis</i> Strains
title_fullStr	Characterization of Four Novel dsRNA Viruses Isolated from <i>Mucor</i> <i>hiemalis</i> Strains
title_full_unstemmed	Characterization of Four Novel dsRNA Viruses Isolated from <i>Mucor</i> <i>hiemalis</i> Strains
title_sort	characterization of four novel dsrna viruses isolated from <i>mucor</i> <i>hiemalis</i> strains
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1d2244daf5f74c28ae08595010a89af5
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Characterization of Four Novel dsRNA Viruses Isolated from <i>Mucor</i> <i>hiemalis</i> Strains

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