Nano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria

Abstract Lantibiotics, bacteria-sourced antimicrobial peptides, are very good candidates for effective and safe food additives. Among them, nisin is already approved by the EU and FDA, and has been used in food preservation for the past 40 years. Now, there is a possibility and strong interest to ex...

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Autores principales:	Marija Vukomanović, Vojka Žunič, Špela Kunej, Boštjan Jančar, Samo Jeverica, Rok Podlipec, Danilo Suvorov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d247103c3174c7fb3adeaed14d5fe7d
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spelling	oai:doaj.org-article:1d247103c3174c7fb3adeaed14d5fe7d2021-12-02T11:52:43ZNano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria10.1038/s41598-017-04670-02045-2322https://doaj.org/article/1d247103c3174c7fb3adeaed14d5fe7d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04670-0https://doaj.org/toc/2045-2322Abstract Lantibiotics, bacteria-sourced antimicrobial peptides, are very good candidates for effective and safe food additives. Among them, nisin is already approved by the EU and FDA, and has been used in food preservation for the past 40 years. Now, there is a possibility and strong interest to extend its applicability to biomedicine for designing innovative alternatives to antibiotics. The main obstacle is, however, its naturally narrow spectrum of antimicrobial activity, focused on Gram positive bacteria. Here we demonstrate broadening nisin’s spectrum to Gram negative bacteria using a nano-engineering approach. After binding nisin molecules to the surface of gold nano-features, uniformly deposited on spherical carbon templates, we created a nanocomposite with a high density of positively charged groups. Before assembly, none of the components of the nanocomposite showed any activity against bacterial growth, which was changed after assembly in the form of the nanocomposite. For the first time we showed that this type of structure enables interactions capable of disintegrating the wall of Gram negative bacteria. As confirmed by the nisin model, the developed approach opens up new horizons for the use of lantibiotics in designing post-antibiotic drugs.Marija VukomanovićVojka ŽuničŠpela KunejBoštjan JančarSamo JevericaRok PodlipecDanilo SuvorovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marija Vukomanović Vojka Žunič Špela Kunej Boštjan Jančar Samo Jeverica Rok Podlipec Danilo Suvorov Nano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria
description	Abstract Lantibiotics, bacteria-sourced antimicrobial peptides, are very good candidates for effective and safe food additives. Among them, nisin is already approved by the EU and FDA, and has been used in food preservation for the past 40 years. Now, there is a possibility and strong interest to extend its applicability to biomedicine for designing innovative alternatives to antibiotics. The main obstacle is, however, its naturally narrow spectrum of antimicrobial activity, focused on Gram positive bacteria. Here we demonstrate broadening nisin’s spectrum to Gram negative bacteria using a nano-engineering approach. After binding nisin molecules to the surface of gold nano-features, uniformly deposited on spherical carbon templates, we created a nanocomposite with a high density of positively charged groups. Before assembly, none of the components of the nanocomposite showed any activity against bacterial growth, which was changed after assembly in the form of the nanocomposite. For the first time we showed that this type of structure enables interactions capable of disintegrating the wall of Gram negative bacteria. As confirmed by the nisin model, the developed approach opens up new horizons for the use of lantibiotics in designing post-antibiotic drugs.
format	article
author	Marija Vukomanović Vojka Žunič Špela Kunej Boštjan Jančar Samo Jeverica Rok Podlipec Danilo Suvorov
author_facet	Marija Vukomanović Vojka Žunič Špela Kunej Boštjan Jančar Samo Jeverica Rok Podlipec Danilo Suvorov
author_sort	Marija Vukomanović
title	Nano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria
title_short	Nano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria
title_full	Nano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria
title_fullStr	Nano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria
title_full_unstemmed	Nano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria
title_sort	nano-engineering the antimicrobial spectrum of lantibiotics: activity of nisin against gram negative bacteria
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1d247103c3174c7fb3adeaed14d5fe7d
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Nano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria

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