Macrophage morphological plasticity and migration is Rac signalling and MMP9 dependant

Abstract In vitro, depending on extracellular matrix (ECM) architecture, macrophages migrate either in amoeboid or mesenchymal mode; while the first is a general trait of leukocytes, the latter is associated with tissue remodelling via Matrix Metalloproteinases (MMPs). To assess whether these stereo...

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Autores principales:	Jana Travnickova, Sandra Nhim, Naoill Abdellaoui, Farida Djouad, Maï Nguyen-Chi, Andrea Parmeggiani, Karima Kissa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d2b380aa4074f5cbe9ec9046620a50e
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spelling	oai:doaj.org-article:1d2b380aa4074f5cbe9ec9046620a50e2021-12-02T15:54:50ZMacrophage morphological plasticity and migration is Rac signalling and MMP9 dependant10.1038/s41598-021-88961-72045-2322https://doaj.org/article/1d2b380aa4074f5cbe9ec9046620a50e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88961-7https://doaj.org/toc/2045-2322Abstract In vitro, depending on extracellular matrix (ECM) architecture, macrophages migrate either in amoeboid or mesenchymal mode; while the first is a general trait of leukocytes, the latter is associated with tissue remodelling via Matrix Metalloproteinases (MMPs). To assess whether these stereotyped migrations could be also observed in a physiological context, we used the zebrafish embryo and monitored macrophage morphology, behaviour and capacity to mobilise haematopoietic stem/progenitor cells (HSPCs), as a final functional readout. Morphometric analysis identified 4 different cell shapes. Live imaging revealed that macrophages successively adopt all four shapes as they migrate through ECM. Treatment with inhibitors of MMPs or Rac GTPase to abolish mesenchymal migration, suppresses both ECM degradation and HSPC mobilisation while differently affecting macrophage behaviour. This study depicts real time macrophage behaviour in a physiological context and reveals extreme reactivity of these cells constantly adapting and switching migratory shapes to achieve HSPCs proper mobilisation.Jana TravnickovaSandra NhimNaoill AbdellaouiFarida DjouadMaï Nguyen-ChiAndrea ParmeggianiKarima KissaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jana Travnickova Sandra Nhim Naoill Abdellaoui Farida Djouad Maï Nguyen-Chi Andrea Parmeggiani Karima Kissa Macrophage morphological plasticity and migration is Rac signalling and MMP9 dependant
description	Abstract In vitro, depending on extracellular matrix (ECM) architecture, macrophages migrate either in amoeboid or mesenchymal mode; while the first is a general trait of leukocytes, the latter is associated with tissue remodelling via Matrix Metalloproteinases (MMPs). To assess whether these stereotyped migrations could be also observed in a physiological context, we used the zebrafish embryo and monitored macrophage morphology, behaviour and capacity to mobilise haematopoietic stem/progenitor cells (HSPCs), as a final functional readout. Morphometric analysis identified 4 different cell shapes. Live imaging revealed that macrophages successively adopt all four shapes as they migrate through ECM. Treatment with inhibitors of MMPs or Rac GTPase to abolish mesenchymal migration, suppresses both ECM degradation and HSPC mobilisation while differently affecting macrophage behaviour. This study depicts real time macrophage behaviour in a physiological context and reveals extreme reactivity of these cells constantly adapting and switching migratory shapes to achieve HSPCs proper mobilisation.
format	article
author	Jana Travnickova Sandra Nhim Naoill Abdellaoui Farida Djouad Maï Nguyen-Chi Andrea Parmeggiani Karima Kissa
author_facet	Jana Travnickova Sandra Nhim Naoill Abdellaoui Farida Djouad Maï Nguyen-Chi Andrea Parmeggiani Karima Kissa
author_sort	Jana Travnickova
title	Macrophage morphological plasticity and migration is Rac signalling and MMP9 dependant
title_short	Macrophage morphological plasticity and migration is Rac signalling and MMP9 dependant
title_full	Macrophage morphological plasticity and migration is Rac signalling and MMP9 dependant
title_fullStr	Macrophage morphological plasticity and migration is Rac signalling and MMP9 dependant
title_full_unstemmed	Macrophage morphological plasticity and migration is Rac signalling and MMP9 dependant
title_sort	macrophage morphological plasticity and migration is rac signalling and mmp9 dependant
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1d2b380aa4074f5cbe9ec9046620a50e
work_keys_str_mv	AT janatravnickova macrophagemorphologicalplasticityandmigrationisracsignallingandmmp9dependant AT sandranhim macrophagemorphologicalplasticityandmigrationisracsignallingandmmp9dependant AT naoillabdellaoui macrophagemorphologicalplasticityandmigrationisracsignallingandmmp9dependant AT faridadjouad macrophagemorphologicalplasticityandmigrationisracsignallingandmmp9dependant AT mainguyenchi macrophagemorphologicalplasticityandmigrationisracsignallingandmmp9dependant AT andreaparmeggiani macrophagemorphologicalplasticityandmigrationisracsignallingandmmp9dependant AT karimakissa macrophagemorphologicalplasticityandmigrationisracsignallingandmmp9dependant
_version_	1718385436584312832

Macrophage morphological plasticity and migration is Rac signalling and MMP9 dependant

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