Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes

Abstract The ability to reliably and precisely deliver picolitre volumes is an important component of biological research. Here we describe a high-performance, low-cost, open hardware pressure ejection system (Openspritzer), which can be constructed from off the shelf components. The device is capab...

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Autores principales:	C. J. Forman, H. Tomes, B. Mbobo, R. J. Burman, M. Jacobs, T Baden, J. V. Raimondo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d30c7d3322840d9baf96af0c2d7ff61
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spelling	oai:doaj.org-article:1d30c7d3322840d9baf96af0c2d7ff612021-12-02T15:04:53ZOpenspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes10.1038/s41598-017-02301-22045-2322https://doaj.org/article/1d30c7d3322840d9baf96af0c2d7ff612017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02301-2https://doaj.org/toc/2045-2322Abstract The ability to reliably and precisely deliver picolitre volumes is an important component of biological research. Here we describe a high-performance, low-cost, open hardware pressure ejection system (Openspritzer), which can be constructed from off the shelf components. The device is capable of delivering minute doses of reagents to a wide range of biological and chemical systems. In this work, we characterise the performance of the device and compare it to a popular commercial system using two-photon fluorescence microscopy. We found that Openspritzer provides the same level of control over delivered reagent dose as the commercial system. Next, we demonstrate the utility of Openspritzer in a series of standard neurobiological applications. First, we used Openspritzer to deliver precise amounts of reagents to hippocampal neurons to elicit time- and dose-precise responses on neuronal voltage. Second, we used Openspritzer to deliver infectious viral and bacterial agents to living tissue. This included viral transfection of hippocampal interneurons with channelrhodopsin for the optogenetic manipulation of hippocampal circuitry with light. We anticipate that due to its high performance and low cost Openspritzer will be of interest to a broad range of researchers working in the life and physical sciences.C. J. FormanH. TomesB. MboboR. J. BurmanM. JacobsT BadenJ. V. RaimondoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q C. J. Forman H. Tomes B. Mbobo R. J. Burman M. Jacobs T Baden J. V. Raimondo Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes
description	Abstract The ability to reliably and precisely deliver picolitre volumes is an important component of biological research. Here we describe a high-performance, low-cost, open hardware pressure ejection system (Openspritzer), which can be constructed from off the shelf components. The device is capable of delivering minute doses of reagents to a wide range of biological and chemical systems. In this work, we characterise the performance of the device and compare it to a popular commercial system using two-photon fluorescence microscopy. We found that Openspritzer provides the same level of control over delivered reagent dose as the commercial system. Next, we demonstrate the utility of Openspritzer in a series of standard neurobiological applications. First, we used Openspritzer to deliver precise amounts of reagents to hippocampal neurons to elicit time- and dose-precise responses on neuronal voltage. Second, we used Openspritzer to deliver infectious viral and bacterial agents to living tissue. This included viral transfection of hippocampal interneurons with channelrhodopsin for the optogenetic manipulation of hippocampal circuitry with light. We anticipate that due to its high performance and low cost Openspritzer will be of interest to a broad range of researchers working in the life and physical sciences.
format	article
author	C. J. Forman H. Tomes B. Mbobo R. J. Burman M. Jacobs T Baden J. V. Raimondo
author_facet	C. J. Forman H. Tomes B. Mbobo R. J. Burman M. Jacobs T Baden J. V. Raimondo
author_sort	C. J. Forman
title	Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes
title_short	Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes
title_full	Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes
title_fullStr	Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes
title_full_unstemmed	Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes
title_sort	openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1d30c7d3322840d9baf96af0c2d7ff61
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Openspritzer: an open hardware pressure ejection system for reliably delivering picolitre volumes

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