Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis

Abstract Numerous biological approaches are available to characterise the mechanisms which govern the formation of human embryonic stem cell (hESC) colonies. To understand how the kinematics of single and pairs of hESCs impact colony formation, we study their mobility characteristics using time-laps...

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Autores principales:	L. E. Wadkin, L. F. Elliot, I. Neganova, N. G. Parker, V. Chichagova, G. Swan, A. Laude, M. Lako, A. Shukurov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d3b0eea7c2b4bea94b00830e6e0e51d
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spelling	oai:doaj.org-article:1d3b0eea7c2b4bea94b00830e6e0e51d2021-12-02T16:08:12ZDynamics of single human embryonic stem cells and their pairs: a quantitative analysis10.1038/s41598-017-00648-02045-2322https://doaj.org/article/1d3b0eea7c2b4bea94b00830e6e0e51d2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00648-0https://doaj.org/toc/2045-2322Abstract Numerous biological approaches are available to characterise the mechanisms which govern the formation of human embryonic stem cell (hESC) colonies. To understand how the kinematics of single and pairs of hESCs impact colony formation, we study their mobility characteristics using time-lapse imaging. We perform a detailed statistical analysis of their speed, survival, directionality, distance travelled and diffusivity. We confirm that single and pairs of cells migrate as a diffusive random walk for at least 7 hours of evolution. We show that the presence of Cell Tracer significantly reduces hESC mobility. Our results open the path to employ the theoretical framework of the diffusive random walk for the prognostic modelling and optimisation of the growth of hESC colonies. Indeed, we employ this random walk model to estimate the seeding density required to minimise the occurrence of hESC colonies arising from more than one founder cell and the minimal cell number needed for successful colony formation. Our prognostic model can be extended to investigate the kinematic behaviour of somatic cells emerging from hESC differentiation and to enable its wide application in phenotyping of pluripotent stem cells for large scale stem cell culture expansion and differentiation platforms.L. E. WadkinL. F. ElliotI. NeganovaN. G. ParkerV. ChichagovaG. SwanA. LaudeM. LakoA. ShukurovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q L. E. Wadkin L. F. Elliot I. Neganova N. G. Parker V. Chichagova G. Swan A. Laude M. Lako A. Shukurov Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis
description	Abstract Numerous biological approaches are available to characterise the mechanisms which govern the formation of human embryonic stem cell (hESC) colonies. To understand how the kinematics of single and pairs of hESCs impact colony formation, we study their mobility characteristics using time-lapse imaging. We perform a detailed statistical analysis of their speed, survival, directionality, distance travelled and diffusivity. We confirm that single and pairs of cells migrate as a diffusive random walk for at least 7 hours of evolution. We show that the presence of Cell Tracer significantly reduces hESC mobility. Our results open the path to employ the theoretical framework of the diffusive random walk for the prognostic modelling and optimisation of the growth of hESC colonies. Indeed, we employ this random walk model to estimate the seeding density required to minimise the occurrence of hESC colonies arising from more than one founder cell and the minimal cell number needed for successful colony formation. Our prognostic model can be extended to investigate the kinematic behaviour of somatic cells emerging from hESC differentiation and to enable its wide application in phenotyping of pluripotent stem cells for large scale stem cell culture expansion and differentiation platforms.
format	article
author	L. E. Wadkin L. F. Elliot I. Neganova N. G. Parker V. Chichagova G. Swan A. Laude M. Lako A. Shukurov
author_facet	L. E. Wadkin L. F. Elliot I. Neganova N. G. Parker V. Chichagova G. Swan A. Laude M. Lako A. Shukurov
author_sort	L. E. Wadkin
title	Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis
title_short	Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis
title_full	Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis
title_fullStr	Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis
title_full_unstemmed	Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis
title_sort	dynamics of single human embryonic stem cells and their pairs: a quantitative analysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1d3b0eea7c2b4bea94b00830e6e0e51d
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Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis

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