Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats

Many medicinal plants are widely grown in South- and Southeast Asia countries. Some of them are traditionally used for treatment of diabetes mellitus such as Andrographis paniculata and Centella asiatica. In the study, we provided fractionated-extracts of A. paniculata and C. asiatica to increase th...

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Autores principales:	Reni Ariastuti, La Ode Muhammad Fitrawan, Agung Endro Nugroho, Suwidjiyo Pramono
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2020
Materias:	a. paniculata c. asiatica antioxidant antidiabetes pancreatic protection Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/1d3b3430b5284d099e6871ae04076547
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spelling	oai:doaj.org-article:1d3b3430b5284d099e6871ae040765472021-11-15T06:08:47ZAntidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats2338-94272338-948610.22146/ijp.1135https://doaj.org/article/1d3b3430b5284d099e6871ae040765472020-12-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1135https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Many medicinal plants are widely grown in South- and Southeast Asia countries. Some of them are traditionally used for treatment of diabetes mellitus such as Andrographis paniculata and Centella asiatica. In the study, we provided fractionated-extracts of A. paniculata and C. asiatica to increase the concentration of their active compounds and eliminate unexpected substances. The aim of the study was to evaluate the antidiabetes effect of the combination of their fractionated-extracts in male neonatal streptozotocin (STZ)-induced diabetic rats. In the study, diabetes was injected intraperitoneally 90mg.kg-1 BWSTZ to two day-old rats. At the age of three months, the rats were administered with the combination of both fractionated-extracts for 14 consecutive days. We evaluated antidiabetes with parameters of blood glucose levels, morphology of pancreatic islet, β-cell density as well as immunohistochemical pancreatic insulin. The levels of MDA, SOD and GPx were also determined about oxidative stress change after treatment with the combination. In the study, the combination succeeded to lower the blood glucose level in neonatal STZ-induced diabetic rats. Inline with fact, improvement of rat pancreatic islets and β cells density, as well as moderate restoration of pancreatic insulin, were observed after treatment with the combination. The substance could decrease the level of MDA, and increase the levels of SOD and GPx. In conclusion, the combination of fractionated-extracts of A. paniculata and C. asiatica exhibited potential antidiabetic effect to its antioxidative effect in male neonatal STZ-induced diabetes rats.Reni AriastutiLa Ode Muhammad FitrawanAgung Endro NugrohoSuwidjiyo PramonoUniversitas Gadjah Madaarticlea. paniculatac. asiaticaantioxidantantidiabetespancreatic protectionPharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 312-322 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	a. paniculata c. asiatica antioxidant antidiabetes pancreatic protection Pharmacy and materia medica RS1-441
spellingShingle	a. paniculata c. asiatica antioxidant antidiabetes pancreatic protection Pharmacy and materia medica RS1-441 Reni Ariastuti La Ode Muhammad Fitrawan Agung Endro Nugroho Suwidjiyo Pramono Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats
description	Many medicinal plants are widely grown in South- and Southeast Asia countries. Some of them are traditionally used for treatment of diabetes mellitus such as Andrographis paniculata and Centella asiatica. In the study, we provided fractionated-extracts of A. paniculata and C. asiatica to increase the concentration of their active compounds and eliminate unexpected substances. The aim of the study was to evaluate the antidiabetes effect of the combination of their fractionated-extracts in male neonatal streptozotocin (STZ)-induced diabetic rats. In the study, diabetes was injected intraperitoneally 90mg.kg-1 BWSTZ to two day-old rats. At the age of three months, the rats were administered with the combination of both fractionated-extracts for 14 consecutive days. We evaluated antidiabetes with parameters of blood glucose levels, morphology of pancreatic islet, β-cell density as well as immunohistochemical pancreatic insulin. The levels of MDA, SOD and GPx were also determined about oxidative stress change after treatment with the combination. In the study, the combination succeeded to lower the blood glucose level in neonatal STZ-induced diabetic rats. Inline with fact, improvement of rat pancreatic islets and β cells density, as well as moderate restoration of pancreatic insulin, were observed after treatment with the combination. The substance could decrease the level of MDA, and increase the levels of SOD and GPx. In conclusion, the combination of fractionated-extracts of A. paniculata and C. asiatica exhibited potential antidiabetic effect to its antioxidative effect in male neonatal STZ-induced diabetes rats.
format	article
author	Reni Ariastuti La Ode Muhammad Fitrawan Agung Endro Nugroho Suwidjiyo Pramono
author_facet	Reni Ariastuti La Ode Muhammad Fitrawan Agung Endro Nugroho Suwidjiyo Pramono
author_sort	Reni Ariastuti
title	Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats
title_short	Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats
title_full	Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats
title_fullStr	Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats
title_full_unstemmed	Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats
title_sort	antidiabetes of combination of fractionated-extracts of andrographis paniculata and centella asiatica in neonatal streptozotocin-induced diabetic rats
publisher	Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/1d3b3430b5284d099e6871ae04076547
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Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats

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