Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms

Abstract With the increasing recognition of biofilms in human disease, the development of novel antimicrobial therapies is of critical importance. For example, in patients with cystic fibrosis (CF), the acquisition of host-adapted, chronic Pseudomonas aeruginosa infection is associated with a declin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Trevor Beaudoin, Tracy A. Stone, Miroslawa Glibowicka, Christina Adams, Yvonne Yau, Saumel Ahmadi, Christine E. Bear, Hartmut Grasemann, Valerie Waters, Charles M. Deber
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Biovolume Multidrug Resistance (MDR) Cationic Antimicrobial Peptides (CAPs) Colony Forming Units Per Ml (CFU/mL) Cystic Fibrosis Bronchial Epithelial Cells (CFBE) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d3bcf416aa84b6eb2bf558f5d4620b3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1d3bcf416aa84b6eb2bf558f5d4620b3
record_format	dspace
spelling	oai:doaj.org-article:1d3bcf416aa84b6eb2bf558f5d4620b32021-12-02T15:08:37ZActivity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms10.1038/s41598-018-33016-72045-2322https://doaj.org/article/1d3bcf416aa84b6eb2bf558f5d4620b32018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33016-7https://doaj.org/toc/2045-2322Abstract With the increasing recognition of biofilms in human disease, the development of novel antimicrobial therapies is of critical importance. For example, in patients with cystic fibrosis (CF), the acquisition of host-adapted, chronic Pseudomonas aeruginosa infection is associated with a decline in lung function and increased mortality. Our objective was to test the in vitro efficacy of a membrane-active antimicrobial peptide we designed, termed 6K-F17 (sequence: KKKKKK-AAFAAWAAFAA-NH2), against multidrug resistant P. aeruginosa biofilms. This peptide displays high antimicrobial activity against a range of pathogenic bacteria, yet is non-hemolytic to human erythrocytes and non-toxic to human bronchial epithelial cells. In the present work, P. aeruginosa strain PAO1, and four multidrug resistant (MDR) isolates from chronically infected CF individuals, were grown as 48-hour biofilms in a static biofilm slide chamber model. These biofilms were then exposed to varying concentrations of 6K-F17 alone, or in the presence of tobramycin, prior to confocal imaging. Biofilm biovolume and viability were assessed. 6K-F17 was able to kill biofilms – even in the presence of sputum – and greatly reduce biofilm biovolume in PAO1 and MDR isolates. Strikingly, when used in conjunction with tobramycin, low doses of 6K-F17 significantly potentiated tobramycin killing, leading to biofilm destruction.Trevor BeaudoinTracy A. StoneMiroslawa GlibowickaChristina AdamsYvonne YauSaumel AhmadiChristine E. BearHartmut GrasemannValerie WatersCharles M. DeberNature PortfolioarticleBiovolumeMultidrug Resistance (MDR)Cationic Antimicrobial Peptides (CAPs)Colony Forming Units Per Ml (CFU/mL)Cystic Fibrosis Bronchial Epithelial Cells (CFBE)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biovolume Multidrug Resistance (MDR) Cationic Antimicrobial Peptides (CAPs) Colony Forming Units Per Ml (CFU/mL) Cystic Fibrosis Bronchial Epithelial Cells (CFBE) Medicine R Science Q
spellingShingle	Biovolume Multidrug Resistance (MDR) Cationic Antimicrobial Peptides (CAPs) Colony Forming Units Per Ml (CFU/mL) Cystic Fibrosis Bronchial Epithelial Cells (CFBE) Medicine R Science Q Trevor Beaudoin Tracy A. Stone Miroslawa Glibowicka Christina Adams Yvonne Yau Saumel Ahmadi Christine E. Bear Hartmut Grasemann Valerie Waters Charles M. Deber Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms
description	Abstract With the increasing recognition of biofilms in human disease, the development of novel antimicrobial therapies is of critical importance. For example, in patients with cystic fibrosis (CF), the acquisition of host-adapted, chronic Pseudomonas aeruginosa infection is associated with a decline in lung function and increased mortality. Our objective was to test the in vitro efficacy of a membrane-active antimicrobial peptide we designed, termed 6K-F17 (sequence: KKKKKK-AAFAAWAAFAA-NH2), against multidrug resistant P. aeruginosa biofilms. This peptide displays high antimicrobial activity against a range of pathogenic bacteria, yet is non-hemolytic to human erythrocytes and non-toxic to human bronchial epithelial cells. In the present work, P. aeruginosa strain PAO1, and four multidrug resistant (MDR) isolates from chronically infected CF individuals, were grown as 48-hour biofilms in a static biofilm slide chamber model. These biofilms were then exposed to varying concentrations of 6K-F17 alone, or in the presence of tobramycin, prior to confocal imaging. Biofilm biovolume and viability were assessed. 6K-F17 was able to kill biofilms – even in the presence of sputum – and greatly reduce biofilm biovolume in PAO1 and MDR isolates. Strikingly, when used in conjunction with tobramycin, low doses of 6K-F17 significantly potentiated tobramycin killing, leading to biofilm destruction.
format	article
author	Trevor Beaudoin Tracy A. Stone Miroslawa Glibowicka Christina Adams Yvonne Yau Saumel Ahmadi Christine E. Bear Hartmut Grasemann Valerie Waters Charles M. Deber
author_facet	Trevor Beaudoin Tracy A. Stone Miroslawa Glibowicka Christina Adams Yvonne Yau Saumel Ahmadi Christine E. Bear Hartmut Grasemann Valerie Waters Charles M. Deber
author_sort	Trevor Beaudoin
title	Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms
title_short	Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms
title_full	Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms
title_fullStr	Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms
title_full_unstemmed	Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms
title_sort	activity of a novel antimicrobial peptide against pseudomonas aeruginosa biofilms
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1d3bcf416aa84b6eb2bf558f5d4620b3
work_keys_str_mv	AT trevorbeaudoin activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms AT tracyastone activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms AT miroslawaglibowicka activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms AT christinaadams activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms AT yvonneyau activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms AT saumelahmadi activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms AT christineebear activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms AT hartmutgrasemann activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms AT valeriewaters activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms AT charlesmdeber activityofanovelantimicrobialpeptideagainstpseudomonasaeruginosabiofilms
_version_	1718388046136606720

Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms

Ejemplares similares