Evolution of Outbreak-Causing Carbapenem-Resistant <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> ST258 at a Tertiary Care Hospital over 8 Years

ABSTRACT Carbapenem-resistant Klebsiella pneumoniae (CRKP) strains belonging to sequence type 258 (ST258) are frequent causes of hospital-associated outbreaks and are a major contributor to the spread of carbapenemases. This genetic lineage emerged several decades ago and remains a major global heal...

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Autores principales:	Jane W. Marsh, Mustapha M. Mustapha, Marissa P. Griffith, Daniel R. Evans, Chinelo Ezeonwuka, A. William Pasculle, Kathleen A. Shutt, Alexander Sundermann, Ashley M. Ayres, Ryan K. Shields, Ahmed Babiker, Vaughn S. Cooper, Daria Van Tyne, Lee H. Harrison
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	carbapenem-resistant evolution genomics molecular epidemiology outbreaks Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/1d4671ba568146ef9aa85a4bfa3eb417
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spelling	oai:doaj.org-article:1d4671ba568146ef9aa85a4bfa3eb4172021-11-15T15:59:42ZEvolution of Outbreak-Causing Carbapenem-Resistant <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> ST258 at a Tertiary Care Hospital over 8 Years10.1128/mBio.01945-192150-7511https://doaj.org/article/1d4671ba568146ef9aa85a4bfa3eb4172019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01945-19https://doaj.org/toc/2150-7511ABSTRACT Carbapenem-resistant Klebsiella pneumoniae (CRKP) strains belonging to sequence type 258 (ST258) are frequent causes of hospital-associated outbreaks and are a major contributor to the spread of carbapenemases. This genetic lineage emerged several decades ago and remains a major global health care challenge. In this study, genomic epidemiology was used to investigate the emergence, evolution, and persistence of ST258 carbapenem-resistant K. pneumoniae outbreak-causing lineages at a large tertiary care hospital over 8 years. A time-based phylogenetic analysis of 136 ST258 isolates demonstrated the succession of multiple genetically distinct ST258 sublineages over the 8-year period. Ongoing genomic surveillance identified the emergence and persistence of several distinct clonal ST258 populations. Patterns of multidrug resistance determinants and plasmid replicons were consistent with continued evolution and persistence of these populations. Five ST258 outbreaks were documented, including three that were caused by the same clonal lineage. Mutations in genes encoding effectors of biofilm production and iron acquisition were identified among persistent clones. Two emergent lineages bearing K. pneumoniae integrative conjugative element 10 (ICEKp10) and harboring yersiniabactin and colibactin virulence factors were identified. The results show how distinct ST258 subpopulations have evolved and persisted within the same hospital over nearly a decade. IMPORTANCE The carbapenem class of antibiotics is invaluable for the treatment of selected multidrug-resistant Gram-negative pathogens. The continued transmission of carbapenem-resistant bacteria such as ST258 K. pneumoniae is of serious global public health concern, as treatment options for these infections are limited. This genomic epidemiologic investigation traced the natural history of ST258 K. pneumoniae in a single health care setting over nearly a decade. We found that distinct ST258 subpopulations have caused both device-associated and ward-associated outbreaks, and some of these populations remain endemic within our hospital to the present day. The finding of virulence determinants among emergent ST258 clones supports the idea of convergent evolution of drug-resistant and virulent CRKP strains and highlights the need for continued surveillance, prevention, and control efforts to address emergent and evolving ST258 populations in the health care setting.Jane W. MarshMustapha M. MustaphaMarissa P. GriffithDaniel R. EvansChinelo EzeonwukaA. William PasculleKathleen A. ShuttAlexander SundermannAshley M. AyresRyan K. ShieldsAhmed BabikerVaughn S. CooperDaria Van TyneLee H. HarrisonAmerican Society for Microbiologyarticlecarbapenem-resistantevolutiongenomicsmolecular epidemiologyoutbreaksMicrobiologyQR1-502ENmBio, Vol 10, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbapenem-resistant evolution genomics molecular epidemiology outbreaks Microbiology QR1-502
spellingShingle	carbapenem-resistant evolution genomics molecular epidemiology outbreaks Microbiology QR1-502 Jane W. Marsh Mustapha M. Mustapha Marissa P. Griffith Daniel R. Evans Chinelo Ezeonwuka A. William Pasculle Kathleen A. Shutt Alexander Sundermann Ashley M. Ayres Ryan K. Shields Ahmed Babiker Vaughn S. Cooper Daria Van Tyne Lee H. Harrison Evolution of Outbreak-Causing Carbapenem-Resistant <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> ST258 at a Tertiary Care Hospital over 8 Years
description	ABSTRACT Carbapenem-resistant Klebsiella pneumoniae (CRKP) strains belonging to sequence type 258 (ST258) are frequent causes of hospital-associated outbreaks and are a major contributor to the spread of carbapenemases. This genetic lineage emerged several decades ago and remains a major global health care challenge. In this study, genomic epidemiology was used to investigate the emergence, evolution, and persistence of ST258 carbapenem-resistant K. pneumoniae outbreak-causing lineages at a large tertiary care hospital over 8 years. A time-based phylogenetic analysis of 136 ST258 isolates demonstrated the succession of multiple genetically distinct ST258 sublineages over the 8-year period. Ongoing genomic surveillance identified the emergence and persistence of several distinct clonal ST258 populations. Patterns of multidrug resistance determinants and plasmid replicons were consistent with continued evolution and persistence of these populations. Five ST258 outbreaks were documented, including three that were caused by the same clonal lineage. Mutations in genes encoding effectors of biofilm production and iron acquisition were identified among persistent clones. Two emergent lineages bearing K. pneumoniae integrative conjugative element 10 (ICEKp10) and harboring yersiniabactin and colibactin virulence factors were identified. The results show how distinct ST258 subpopulations have evolved and persisted within the same hospital over nearly a decade. IMPORTANCE The carbapenem class of antibiotics is invaluable for the treatment of selected multidrug-resistant Gram-negative pathogens. The continued transmission of carbapenem-resistant bacteria such as ST258 K. pneumoniae is of serious global public health concern, as treatment options for these infections are limited. This genomic epidemiologic investigation traced the natural history of ST258 K. pneumoniae in a single health care setting over nearly a decade. We found that distinct ST258 subpopulations have caused both device-associated and ward-associated outbreaks, and some of these populations remain endemic within our hospital to the present day. The finding of virulence determinants among emergent ST258 clones supports the idea of convergent evolution of drug-resistant and virulent CRKP strains and highlights the need for continued surveillance, prevention, and control efforts to address emergent and evolving ST258 populations in the health care setting.
format	article
author	Jane W. Marsh Mustapha M. Mustapha Marissa P. Griffith Daniel R. Evans Chinelo Ezeonwuka A. William Pasculle Kathleen A. Shutt Alexander Sundermann Ashley M. Ayres Ryan K. Shields Ahmed Babiker Vaughn S. Cooper Daria Van Tyne Lee H. Harrison
author_facet	Jane W. Marsh Mustapha M. Mustapha Marissa P. Griffith Daniel R. Evans Chinelo Ezeonwuka A. William Pasculle Kathleen A. Shutt Alexander Sundermann Ashley M. Ayres Ryan K. Shields Ahmed Babiker Vaughn S. Cooper Daria Van Tyne Lee H. Harrison
author_sort	Jane W. Marsh
title	Evolution of Outbreak-Causing Carbapenem-Resistant <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> ST258 at a Tertiary Care Hospital over 8 Years
title_short	Evolution of Outbreak-Causing Carbapenem-Resistant <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> ST258 at a Tertiary Care Hospital over 8 Years
title_full	Evolution of Outbreak-Causing Carbapenem-Resistant <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> ST258 at a Tertiary Care Hospital over 8 Years
title_fullStr	Evolution of Outbreak-Causing Carbapenem-Resistant <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> ST258 at a Tertiary Care Hospital over 8 Years
title_full_unstemmed	Evolution of Outbreak-Causing Carbapenem-Resistant <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> ST258 at a Tertiary Care Hospital over 8 Years
title_sort	evolution of outbreak-causing carbapenem-resistant <named-content content-type="genus-species">klebsiella pneumoniae</named-content> st258 at a tertiary care hospital over 8 years
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/1d4671ba568146ef9aa85a4bfa3eb417
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Evolution of Outbreak-Causing Carbapenem-Resistant <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> ST258 at a Tertiary Care Hospital over 8 Years

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