Effect of Paraoxon on the Oxidative Stress Indices and Lactate Dehydrogenase Activity in Rat Liver

BACKGROUND AND OBJECTIVE: Paraoxon (active metabolite of parathion) is one of the most toxic organophosphate (OP) compounds. Acetyl cholinesterase inhibition is the main acute toxic mechanisms of OPs. Free radicals production and disruption of antioxidant defense systems is one of non-cholinergic ef...

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Autores principales:	E Ghani, M Mohammadi, M Jafari, A Khoshbaten, AR Asgari
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2012
Materias:	paraoxon oxidative stress liver lactate dehydrogenase rat Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1d4c5a6751d04d34accc20baa00f480c
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spelling	oai:doaj.org-article:1d4c5a6751d04d34accc20baa00f480c2021-11-10T08:52:02ZEffect of Paraoxon on the Oxidative Stress Indices and Lactate Dehydrogenase Activity in Rat Liver1561-41072251-7170https://doaj.org/article/1d4c5a6751d04d34accc20baa00f480c2012-01-01T00:00:00Zhttp://jbums.org/article-1-3982-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Paraoxon (active metabolite of parathion) is one of the most toxic organophosphate (OP) compounds. Acetyl cholinesterase inhibition is the main acute toxic mechanisms of OPs. Free radicals production and disruption of antioxidant defense systems is one of non-cholinergic effects of these compounds. In the present study, the effect of acute paraoxon administration on the oxidative stress indices in rat liver and lactate dehydrogenase (LDH) activity in plasma and liver were evaluated.METHODS: In the present study, 32 male Wistar rats (200-250 g) were randomly divided into 4 groups (6 animals in each group): control (corn oil as paraoxon solvent) and three experimental groups received different doses of paraoxon (0.07, 0.35, or 0.7 mg/kg body weight) intraperitoneally. Four hours after injection, under ether anesthesia, blood sample is given, liver tissue removed and transferred to liquid nitrogen. Superoxide dismutase (SOD), catalase (CAT), and LDH activities and the levels of glutathione (GSH) and malondialdehyde (MDA) were determined in liver tissue homogenates.FINDINGS: Significant increase in SOD activity and decrease in CAT activity at doses of 0.35 and 0.7 mg/kg of paraoxon were observed (p<0.05). The level of glutathione significantly decreased in all doses of paraoxon. Paraoxon in all treatments induced significant increase in plasma LDH activity and decrease in liver LDH activity. MDA concentration did not show any significant alteration.CONCLUSION: Present findings indicate that acute OP administration induces free radical generation, impairment of cell antioxidant balance and predispose the cells to oxidative stress. So measurement of plasma LDH activity might be a beneficial marker in OP toxicity.E GhaniM MohammadiM JafariA KhoshbatenAR AsgariBabol University of Medical Sciencesarticleparaoxonoxidative stressliverlactate dehydrogenaseratMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 1, Pp 45-52 (2012)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	paraoxon oxidative stress liver lactate dehydrogenase rat Medicine R Medicine (General) R5-920
spellingShingle	paraoxon oxidative stress liver lactate dehydrogenase rat Medicine R Medicine (General) R5-920 E Ghani M Mohammadi M Jafari A Khoshbaten AR Asgari Effect of Paraoxon on the Oxidative Stress Indices and Lactate Dehydrogenase Activity in Rat Liver
description	BACKGROUND AND OBJECTIVE: Paraoxon (active metabolite of parathion) is one of the most toxic organophosphate (OP) compounds. Acetyl cholinesterase inhibition is the main acute toxic mechanisms of OPs. Free radicals production and disruption of antioxidant defense systems is one of non-cholinergic effects of these compounds. In the present study, the effect of acute paraoxon administration on the oxidative stress indices in rat liver and lactate dehydrogenase (LDH) activity in plasma and liver were evaluated.METHODS: In the present study, 32 male Wistar rats (200-250 g) were randomly divided into 4 groups (6 animals in each group): control (corn oil as paraoxon solvent) and three experimental groups received different doses of paraoxon (0.07, 0.35, or 0.7 mg/kg body weight) intraperitoneally. Four hours after injection, under ether anesthesia, blood sample is given, liver tissue removed and transferred to liquid nitrogen. Superoxide dismutase (SOD), catalase (CAT), and LDH activities and the levels of glutathione (GSH) and malondialdehyde (MDA) were determined in liver tissue homogenates.FINDINGS: Significant increase in SOD activity and decrease in CAT activity at doses of 0.35 and 0.7 mg/kg of paraoxon were observed (p<0.05). The level of glutathione significantly decreased in all doses of paraoxon. Paraoxon in all treatments induced significant increase in plasma LDH activity and decrease in liver LDH activity. MDA concentration did not show any significant alteration.CONCLUSION: Present findings indicate that acute OP administration induces free radical generation, impairment of cell antioxidant balance and predispose the cells to oxidative stress. So measurement of plasma LDH activity might be a beneficial marker in OP toxicity.
format	article
author	E Ghani M Mohammadi M Jafari A Khoshbaten AR Asgari
author_facet	E Ghani M Mohammadi M Jafari A Khoshbaten AR Asgari
author_sort	E Ghani
title	Effect of Paraoxon on the Oxidative Stress Indices and Lactate Dehydrogenase Activity in Rat Liver
title_short	Effect of Paraoxon on the Oxidative Stress Indices and Lactate Dehydrogenase Activity in Rat Liver
title_full	Effect of Paraoxon on the Oxidative Stress Indices and Lactate Dehydrogenase Activity in Rat Liver
title_fullStr	Effect of Paraoxon on the Oxidative Stress Indices and Lactate Dehydrogenase Activity in Rat Liver
title_full_unstemmed	Effect of Paraoxon on the Oxidative Stress Indices and Lactate Dehydrogenase Activity in Rat Liver
title_sort	effect of paraoxon on the oxidative stress indices and lactate dehydrogenase activity in rat liver
publisher	Babol University of Medical Sciences
publishDate	2012
url	https://doaj.org/article/1d4c5a6751d04d34accc20baa00f480c
work_keys_str_mv	AT eghani effectofparaoxonontheoxidativestressindicesandlactatedehydrogenaseactivityinratliver AT mmohammadi effectofparaoxonontheoxidativestressindicesandlactatedehydrogenaseactivityinratliver AT mjafari effectofparaoxonontheoxidativestressindicesandlactatedehydrogenaseactivityinratliver AT akhoshbaten effectofparaoxonontheoxidativestressindicesandlactatedehydrogenaseactivityinratliver AT arasgari effectofparaoxonontheoxidativestressindicesandlactatedehydrogenaseactivityinratliver
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Effect of Paraoxon on the Oxidative Stress Indices and Lactate Dehydrogenase Activity in Rat Liver

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