Chyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers

The time-tested Ayurvedic medicinal food, Chyawanprash, has been a part of the Indian diet since ancient times. It is an extremely concentrated mixture of extracts from medicinal herbs and processed minerals, known for its immunity boosting, rejuvenating, and anti-oxidative effects. In this study, w...

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Autores principales:	Acharya Balkrishna, Meenu Tomer, Moumita Manik, Jyotish Srivastava, Rishabh Dev, Swati Haldar, Anurag Varshney
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	chyawanprash inflammation zebrafish health supplement medicinal food nutraceutical Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/1d503eae3a244a99bd9f778bff8ca485
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spelling	oai:doaj.org-article:1d503eae3a244a99bd9f778bff8ca4852021-11-12T06:17:49ZChyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers1663-981210.3389/fphar.2021.751576https://doaj.org/article/1d503eae3a244a99bd9f778bff8ca4852021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.751576/fullhttps://doaj.org/toc/1663-9812The time-tested Ayurvedic medicinal food, Chyawanprash, has been a part of the Indian diet since ancient times. It is an extremely concentrated mixture of extracts from medicinal herbs and processed minerals, known for its immunity boosting, rejuvenating, and anti-oxidative effects. In this study, we have evaluated the anti-inflammatory potential of Patanjali Special Chyawanprash (PSCP) using the zebrafish model of inflammation. Zebrafish were fed on PSCP-infused pellets at stipulated doses for 13 days before inducing inflammation through lipopolysaccharide (LPS) injection. The test subjects were monitored for inflammatory pathologies like behavioral fever, hyperventilation, skin hemorrhage, locomotory agility, and morphological anomaly. PSCP exerted a strong prophylactic effect on the zebrafish that efficiently protected them from inflammatory manifestations at a human equivalent dose. Expression levels of pro-inflammatory cytokines, like interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and interleukin-1 beta (IL-1β), were also reduced in the LPS-stimulated zebrafish fed on PSCP-infused pellets. Skin hemorrhage, hyperventilation, and loss of caudal fins are characteristics of LPS-induced inflammation in zebrafish. PSCP prophylactically ameliorated skin hemorrhage, restored normal respiration, and prevented loss of caudal fin in inflamed zebrafish. Under in vitro conditions, PSCP reduced IL-6 and TNF-α secretion by THP-1 macrophages in a dose-dependent manner by targeting NF-κB signaling, as evident from the secreted embryonic alkaline phosphatase (SEAP) reporter assay. These medicinal benefits of PSCP can be attributed to its constitutional bioactive components. Taken together, these observations provide in vivo validation of the anti-inflammatory property and in vitro insight into the mode-of-action of Chyawanprash, a traditionally described medicinal food.Acharya BalkrishnaAcharya BalkrishnaMeenu TomerMoumita ManikJyotish SrivastavaRishabh DevSwati HaldarAnurag VarshneyAnurag VarshneyAnurag VarshneyFrontiers Media S.A.articlechyawanprashinflammationzebrafishhealth supplementmedicinal foodnutraceuticalTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chyawanprash inflammation zebrafish health supplement medicinal food nutraceutical Therapeutics. Pharmacology RM1-950
spellingShingle	chyawanprash inflammation zebrafish health supplement medicinal food nutraceutical Therapeutics. Pharmacology RM1-950 Acharya Balkrishna Acharya Balkrishna Meenu Tomer Moumita Manik Jyotish Srivastava Rishabh Dev Swati Haldar Anurag Varshney Anurag Varshney Anurag Varshney Chyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers
description	The time-tested Ayurvedic medicinal food, Chyawanprash, has been a part of the Indian diet since ancient times. It is an extremely concentrated mixture of extracts from medicinal herbs and processed minerals, known for its immunity boosting, rejuvenating, and anti-oxidative effects. In this study, we have evaluated the anti-inflammatory potential of Patanjali Special Chyawanprash (PSCP) using the zebrafish model of inflammation. Zebrafish were fed on PSCP-infused pellets at stipulated doses for 13 days before inducing inflammation through lipopolysaccharide (LPS) injection. The test subjects were monitored for inflammatory pathologies like behavioral fever, hyperventilation, skin hemorrhage, locomotory agility, and morphological anomaly. PSCP exerted a strong prophylactic effect on the zebrafish that efficiently protected them from inflammatory manifestations at a human equivalent dose. Expression levels of pro-inflammatory cytokines, like interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and interleukin-1 beta (IL-1β), were also reduced in the LPS-stimulated zebrafish fed on PSCP-infused pellets. Skin hemorrhage, hyperventilation, and loss of caudal fins are characteristics of LPS-induced inflammation in zebrafish. PSCP prophylactically ameliorated skin hemorrhage, restored normal respiration, and prevented loss of caudal fin in inflamed zebrafish. Under in vitro conditions, PSCP reduced IL-6 and TNF-α secretion by THP-1 macrophages in a dose-dependent manner by targeting NF-κB signaling, as evident from the secreted embryonic alkaline phosphatase (SEAP) reporter assay. These medicinal benefits of PSCP can be attributed to its constitutional bioactive components. Taken together, these observations provide in vivo validation of the anti-inflammatory property and in vitro insight into the mode-of-action of Chyawanprash, a traditionally described medicinal food.
format	article
author	Acharya Balkrishna Acharya Balkrishna Meenu Tomer Moumita Manik Jyotish Srivastava Rishabh Dev Swati Haldar Anurag Varshney Anurag Varshney Anurag Varshney
author_facet	Acharya Balkrishna Acharya Balkrishna Meenu Tomer Moumita Manik Jyotish Srivastava Rishabh Dev Swati Haldar Anurag Varshney Anurag Varshney Anurag Varshney
author_sort	Acharya Balkrishna
title	Chyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers
title_short	Chyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers
title_full	Chyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers
title_fullStr	Chyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers
title_full_unstemmed	Chyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers
title_sort	chyawanprash, an ancient indian ayurvedic medicinal food, regulates immune response in zebrafish model of inflammation by moderating inflammatory biomarkers
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1d503eae3a244a99bd9f778bff8ca485
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Chyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers

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