The active human gut microbiota differs from the total microbiota.

The human gut microbiota is considered one of the most fascinating reservoirs of microbial diversity hosting between 400 to 1000 bacterial species distributed among nine phyla with Firmicutes, Bacteroidetes and Actinobacteria representing around 75% of the diversity. One of the most intriguing issue...

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Autores principales:	Francesc Peris-Bondia, Amparo Latorre, Alejandro Artacho, Andrés Moya, Giuseppe D'Auria
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d5ee4eeb8354a6e9317a95573a5bf17
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spelling	oai:doaj.org-article:1d5ee4eeb8354a6e9317a95573a5bf172021-11-18T06:49:11ZThe active human gut microbiota differs from the total microbiota.1932-620310.1371/journal.pone.0022448https://doaj.org/article/1d5ee4eeb8354a6e9317a95573a5bf172011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21829462/?tool=EBIhttps://doaj.org/toc/1932-6203The human gut microbiota is considered one of the most fascinating reservoirs of microbial diversity hosting between 400 to 1000 bacterial species distributed among nine phyla with Firmicutes, Bacteroidetes and Actinobacteria representing around 75% of the diversity. One of the most intriguing issues relates to understanding which microbial groups are active players in the maintenance of the microbiota homeostasis.Here, we describe the diversity of active microbial fractions compared with the whole community from raw human fecal samples. We studied four healthy volunteers by 16S rDNA gene pyrosequencing. The fractions were obtained by cell sorting based on bacterial RNA concentration. Bacterial families were observed to appear or disappear on applying a cell sorting method in which flow cytometry was used to evaluate the active cells by pyronin-Y staining of RNA. This method was able to detect active bacteria, indicating that the active players differed from that observed in raw fecal material. Generally, observations showed that in the active fractions, the number of reads related to Bacteroidetes decreased whereas several families from Clostridiales (Firmicutes) were more highly represented. Moreover, a huge number of families appeared as part of the active fraction when cell sorting was applied, indicating reads that are simply statistically hidden by the total reads.Francesc Peris-BondiaAmparo LatorreAlejandro ArtachoAndrés MoyaGiuseppe D'AuriaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22448 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesc Peris-Bondia Amparo Latorre Alejandro Artacho Andrés Moya Giuseppe D'Auria The active human gut microbiota differs from the total microbiota.
description	The human gut microbiota is considered one of the most fascinating reservoirs of microbial diversity hosting between 400 to 1000 bacterial species distributed among nine phyla with Firmicutes, Bacteroidetes and Actinobacteria representing around 75% of the diversity. One of the most intriguing issues relates to understanding which microbial groups are active players in the maintenance of the microbiota homeostasis.Here, we describe the diversity of active microbial fractions compared with the whole community from raw human fecal samples. We studied four healthy volunteers by 16S rDNA gene pyrosequencing. The fractions were obtained by cell sorting based on bacterial RNA concentration. Bacterial families were observed to appear or disappear on applying a cell sorting method in which flow cytometry was used to evaluate the active cells by pyronin-Y staining of RNA. This method was able to detect active bacteria, indicating that the active players differed from that observed in raw fecal material. Generally, observations showed that in the active fractions, the number of reads related to Bacteroidetes decreased whereas several families from Clostridiales (Firmicutes) were more highly represented. Moreover, a huge number of families appeared as part of the active fraction when cell sorting was applied, indicating reads that are simply statistically hidden by the total reads.
format	article
author	Francesc Peris-Bondia Amparo Latorre Alejandro Artacho Andrés Moya Giuseppe D'Auria
author_facet	Francesc Peris-Bondia Amparo Latorre Alejandro Artacho Andrés Moya Giuseppe D'Auria
author_sort	Francesc Peris-Bondia
title	The active human gut microbiota differs from the total microbiota.
title_short	The active human gut microbiota differs from the total microbiota.
title_full	The active human gut microbiota differs from the total microbiota.
title_fullStr	The active human gut microbiota differs from the total microbiota.
title_full_unstemmed	The active human gut microbiota differs from the total microbiota.
title_sort	active human gut microbiota differs from the total microbiota.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/1d5ee4eeb8354a6e9317a95573a5bf17
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The active human gut microbiota differs from the total microbiota.

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