The mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects

Jingjing Dong,1,* Geng Cui,2,* Long Bi,1,* Jie Li,3 Wei Lei11Institute of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China; 2Institute of Orthopedics, General Hospital of PLA, Beijing, People’s Republic of Chin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dong J, Cui G, Bi L, Li J, Lei W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1d5f85f249a64b7ea6207d90e80a6bdc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1d5f85f249a64b7ea6207d90e80a6bdc
record_format	dspace
spelling	oai:doaj.org-article:1d5f85f249a64b7ea6207d90e80a6bdc2021-12-02T02:31:35ZThe mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects1176-91141178-2013https://doaj.org/article/1d5f85f249a64b7ea6207d90e80a6bdc2013-03-01T00:00:00Zhttp://www.dovepress.com/the-mechanical-and-biological-studies-of-calcium-phosphate-cement-fibr-a12623https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jingjing Dong,1,* Geng Cui,2,* Long Bi,1,* Jie Li,3 Wei Lei11Institute of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China; 2Institute of Orthopedics, General Hospital of PLA, Beijing, People’s Republic of China; 3Institute of Gynecology and Obstetrics, General Hospital of PLA, Beijing, People’s Republic of China*These authors contributed equally to this workAbstract: In order to improve the mechanical and biological properties of calcium phosphate cement (CPC, nanometer-biomaterial) for bone reconstruction in the rabbit femoral defect model, fibrin glue (FG, the natural product, purified from the blood) was introduced at three different ratios. The CPC powder and the FG solution were mixed, respectively, at the powder/liquid (P/L) ratios (g/mL) of 1:1, 3:1, and 5:1 (g/mL), and pure CPC was used as a control. After being implanted into the femoral defect in rabbit, the healing process was evaluated by micro-computed tomography scan, biomechanical testing, and histological examination. By micro-computed tomography analysis, the P/L ratio of 1:1 (g/mL) group indicated the largest quantity of new bone formation at 4 weeks, 8 weeks, and 12 weeks after implantation, respectively. Bone volume per trabecular volume of the 1:1 group was highest in the four groups, which was 1.45% ± 0.42%, 7.35% ± 1.45%, and 29.10% ± 1.67% at 4 weeks, 8 weeks, and 12 weeks after the operation, respectively. In the biomechanical tests, the compressive strength and the elastic modulus of the three CPC–FG groups were much higher than those of the pure CPC group at the determined time point (P < 0.05). The histological evaluation also showed the best osseointegration in the 1:1 group at 4 weeks, 8 weeks, and 12 weeks after the operation, respectively. In the 1:1 group, the bone grew into the pore of the cement in the laminar arrangement and connected with the cement tightly at the 12th week after the operation. This present study indicated that the CPC–FG composite at the P/L ratio of 1:1 (g/mL) stimulated bone regeneration better than any other designed group, which suggested that CPC–FG at the P/L ratio of 1:1 has significant potential as the bioactive material for the treatment of bone defects.Keywords: calcium phosphate cements, fibrin glue, bone reconstruction, micro-CT, biomechanical testing, histological examinationDong JCui GBi LLi JLei WDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1317-1324 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Dong J Cui G Bi L Li J Lei W The mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects
description	Jingjing Dong,1,* Geng Cui,2,* Long Bi,1,* Jie Li,3 Wei Lei11Institute of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China; 2Institute of Orthopedics, General Hospital of PLA, Beijing, People’s Republic of China; 3Institute of Gynecology and Obstetrics, General Hospital of PLA, Beijing, People’s Republic of China*These authors contributed equally to this workAbstract: In order to improve the mechanical and biological properties of calcium phosphate cement (CPC, nanometer-biomaterial) for bone reconstruction in the rabbit femoral defect model, fibrin glue (FG, the natural product, purified from the blood) was introduced at three different ratios. The CPC powder and the FG solution were mixed, respectively, at the powder/liquid (P/L) ratios (g/mL) of 1:1, 3:1, and 5:1 (g/mL), and pure CPC was used as a control. After being implanted into the femoral defect in rabbit, the healing process was evaluated by micro-computed tomography scan, biomechanical testing, and histological examination. By micro-computed tomography analysis, the P/L ratio of 1:1 (g/mL) group indicated the largest quantity of new bone formation at 4 weeks, 8 weeks, and 12 weeks after implantation, respectively. Bone volume per trabecular volume of the 1:1 group was highest in the four groups, which was 1.45% ± 0.42%, 7.35% ± 1.45%, and 29.10% ± 1.67% at 4 weeks, 8 weeks, and 12 weeks after the operation, respectively. In the biomechanical tests, the compressive strength and the elastic modulus of the three CPC–FG groups were much higher than those of the pure CPC group at the determined time point (P < 0.05). The histological evaluation also showed the best osseointegration in the 1:1 group at 4 weeks, 8 weeks, and 12 weeks after the operation, respectively. In the 1:1 group, the bone grew into the pore of the cement in the laminar arrangement and connected with the cement tightly at the 12th week after the operation. This present study indicated that the CPC–FG composite at the P/L ratio of 1:1 (g/mL) stimulated bone regeneration better than any other designed group, which suggested that CPC–FG at the P/L ratio of 1:1 has significant potential as the bioactive material for the treatment of bone defects.Keywords: calcium phosphate cements, fibrin glue, bone reconstruction, micro-CT, biomechanical testing, histological examination
format	article
author	Dong J Cui G Bi L Li J Lei W
author_facet	Dong J Cui G Bi L Li J Lei W
author_sort	Dong J
title	The mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects
title_short	The mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects
title_full	The mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects
title_fullStr	The mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects
title_full_unstemmed	The mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects
title_sort	mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/1d5f85f249a64b7ea6207d90e80a6bdc
work_keys_str_mv	AT dongj themechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects AT cuig themechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects AT bil themechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects AT lij themechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects AT leiw themechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects AT dongj mechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects AT cuig mechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects AT bil mechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects AT lij mechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects AT leiw mechanicalandbiologicalstudiesofcalciumphosphatecementfibringlueforbonereconstructionofrabbitfemoraldefects
_version_	1718402395605565440

The mechanical and biological studies of calcium phosphate cement-fibrin glue for bone reconstruction of rabbit femoral defects

Ejemplares similares