Biofilm induced tolerance towards antimicrobial peptides.

Increased tolerance to antimicrobial agents is thought to be an important feature of microbes growing in biofilms. We address the question of how biofilm organization affects antibiotic susceptibility. We established Escherichia coli biofilms with differential structural organization due to the pres...

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Autores principales:	Anders Folkesson, Janus A J Haagensen, Claudia Zampaloni, Claus Sternberg, Søren Molin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d62c341158f431fbc6a6a50eb630616
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spelling	oai:doaj.org-article:1d62c341158f431fbc6a6a50eb6306162021-11-25T06:12:55ZBiofilm induced tolerance towards antimicrobial peptides.1932-620310.1371/journal.pone.0001891https://doaj.org/article/1d62c341158f431fbc6a6a50eb6306162008-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18382672/?tool=EBIhttps://doaj.org/toc/1932-6203Increased tolerance to antimicrobial agents is thought to be an important feature of microbes growing in biofilms. We address the question of how biofilm organization affects antibiotic susceptibility. We established Escherichia coli biofilms with differential structural organization due to the presence of IncF plasmids expressing altered forms of the transfer pili in two different biofilm model systems. The mature biofilms were subsequently treated with two antibiotics with different molecular targets, the peptide antibiotic colistin and the fluoroquinolone ciprofloxacin. The dynamics of microbial killing were monitored by viable count determination, and confocal laser microscopy. Strains forming structurally organized biofilms show an increased bacterial survival when challenged with colistin, compared to strains forming unstructured biofilms. The increased survival is due to genetically regulated tolerant subpopulation formation and not caused by a general biofilm property. No significant difference in survival was detected when the strains were challenged with ciprofloxacin. Our data show that biofilm formation confers increased colistin tolerance to cells within the biofilm structure, but the protection is conditional being dependent on the structural organization of the biofilm, and the induction of specific tolerance mechanisms.Anders FolkessonJanus A J HaagensenClaudia ZampaloniClaus SternbergSøren MolinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 4, p e1891 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anders Folkesson Janus A J Haagensen Claudia Zampaloni Claus Sternberg Søren Molin Biofilm induced tolerance towards antimicrobial peptides.
description	Increased tolerance to antimicrobial agents is thought to be an important feature of microbes growing in biofilms. We address the question of how biofilm organization affects antibiotic susceptibility. We established Escherichia coli biofilms with differential structural organization due to the presence of IncF plasmids expressing altered forms of the transfer pili in two different biofilm model systems. The mature biofilms were subsequently treated with two antibiotics with different molecular targets, the peptide antibiotic colistin and the fluoroquinolone ciprofloxacin. The dynamics of microbial killing were monitored by viable count determination, and confocal laser microscopy. Strains forming structurally organized biofilms show an increased bacterial survival when challenged with colistin, compared to strains forming unstructured biofilms. The increased survival is due to genetically regulated tolerant subpopulation formation and not caused by a general biofilm property. No significant difference in survival was detected when the strains were challenged with ciprofloxacin. Our data show that biofilm formation confers increased colistin tolerance to cells within the biofilm structure, but the protection is conditional being dependent on the structural organization of the biofilm, and the induction of specific tolerance mechanisms.
format	article
author	Anders Folkesson Janus A J Haagensen Claudia Zampaloni Claus Sternberg Søren Molin
author_facet	Anders Folkesson Janus A J Haagensen Claudia Zampaloni Claus Sternberg Søren Molin
author_sort	Anders Folkesson
title	Biofilm induced tolerance towards antimicrobial peptides.
title_short	Biofilm induced tolerance towards antimicrobial peptides.
title_full	Biofilm induced tolerance towards antimicrobial peptides.
title_fullStr	Biofilm induced tolerance towards antimicrobial peptides.
title_full_unstemmed	Biofilm induced tolerance towards antimicrobial peptides.
title_sort	biofilm induced tolerance towards antimicrobial peptides.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/1d62c341158f431fbc6a6a50eb630616
work_keys_str_mv	AT andersfolkesson biofilminducedtolerancetowardsantimicrobialpeptides AT janusajhaagensen biofilminducedtolerancetowardsantimicrobialpeptides AT claudiazampaloni biofilminducedtolerancetowardsantimicrobialpeptides AT claussternberg biofilminducedtolerancetowardsantimicrobialpeptides AT sørenmolin biofilminducedtolerancetowardsantimicrobialpeptides
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Biofilm induced tolerance towards antimicrobial peptides.

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