Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.

We have sequenced the gene clusters for type strains of the Acinetobacter baumannii serotyping scheme developed in the 1990s, and used the sequences to better understand diversity in surface polysaccharides of the genus. We obtained genome sequences for 27 available serovar type strains, and identif...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dalong Hu, Bin Liu, Lenie Dijkshoorn, Lei Wang, Peter R Reeves
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1d6360c2a1794349838800e8ad14e5d9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1d6360c2a1794349838800e8ad14e5d9
record_format	dspace
spelling	oai:doaj.org-article:1d6360c2a1794349838800e8ad14e5d92021-11-18T09:02:11ZDiversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.1932-620310.1371/journal.pone.0070329https://doaj.org/article/1d6360c2a1794349838800e8ad14e5d92013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23922982/?tool=EBIhttps://doaj.org/toc/1932-6203We have sequenced the gene clusters for type strains of the Acinetobacter baumannii serotyping scheme developed in the 1990s, and used the sequences to better understand diversity in surface polysaccharides of the genus. We obtained genome sequences for 27 available serovar type strains, and identified 25 polysaccharide gene cluster sequences. There are structures for 12 of these polysaccharides, and in general the genes present are appropriate to the structure where known. This greatly facilitates interpretation. We also find 53 different glycosyltransferase genes, and for 7 strains can provisionally allocate specific genes to all linkages. We identified primers that will distinguish the 25 sequence forms by PCR or microarray, or alternatively the genes can be used to determine serotype by "molecular serology". We applied the latter to 190 Acinetobacter genome-derived gene-clusters, and found 76 that have one of the 25 gene-cluster forms. We also found novel gene clusters and added 52 new gene-cluster sequence forms with different wzy genes and different gene contents. Altogether, the strains that have one of the original 25 sequence forms include 98 A. baumannii (24 from our strains) and 5 A. nosocomialis (3 from our strains), whereas 32 genomes from 12 species other than A. baumannii or A. nosocomialis, all have new sequence forms. One of the 25 serovar type sequences is found to be in European clone I (EC I), 2 are in EC II but none in EC III. The public genome strains add an additional 52 new sequence forms, and also bring the number found in EC I to 5, in EC II to 9 and in EC III to 2.Dalong HuBin LiuLenie DijkshoornLei WangPeter R ReevesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e70329 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dalong Hu Bin Liu Lenie Dijkshoorn Lei Wang Peter R Reeves Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.
description	We have sequenced the gene clusters for type strains of the Acinetobacter baumannii serotyping scheme developed in the 1990s, and used the sequences to better understand diversity in surface polysaccharides of the genus. We obtained genome sequences for 27 available serovar type strains, and identified 25 polysaccharide gene cluster sequences. There are structures for 12 of these polysaccharides, and in general the genes present are appropriate to the structure where known. This greatly facilitates interpretation. We also find 53 different glycosyltransferase genes, and for 7 strains can provisionally allocate specific genes to all linkages. We identified primers that will distinguish the 25 sequence forms by PCR or microarray, or alternatively the genes can be used to determine serotype by "molecular serology". We applied the latter to 190 Acinetobacter genome-derived gene-clusters, and found 76 that have one of the 25 gene-cluster forms. We also found novel gene clusters and added 52 new gene-cluster sequence forms with different wzy genes and different gene contents. Altogether, the strains that have one of the original 25 sequence forms include 98 A. baumannii (24 from our strains) and 5 A. nosocomialis (3 from our strains), whereas 32 genomes from 12 species other than A. baumannii or A. nosocomialis, all have new sequence forms. One of the 25 serovar type sequences is found to be in European clone I (EC I), 2 are in EC II but none in EC III. The public genome strains add an additional 52 new sequence forms, and also bring the number found in EC I to 5, in EC II to 9 and in EC III to 2.
format	article
author	Dalong Hu Bin Liu Lenie Dijkshoorn Lei Wang Peter R Reeves
author_facet	Dalong Hu Bin Liu Lenie Dijkshoorn Lei Wang Peter R Reeves
author_sort	Dalong Hu
title	Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.
title_short	Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.
title_full	Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.
title_fullStr	Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.
title_full_unstemmed	Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.
title_sort	diversity in the major polysaccharide antigen of acinetobacter baumannii assessed by dna sequencing, and development of a molecular serotyping scheme.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1d6360c2a1794349838800e8ad14e5d9
work_keys_str_mv	AT dalonghu diversityinthemajorpolysaccharideantigenofacinetobacterbaumanniiassessedbydnasequencinganddevelopmentofamolecularserotypingscheme AT binliu diversityinthemajorpolysaccharideantigenofacinetobacterbaumanniiassessedbydnasequencinganddevelopmentofamolecularserotypingscheme AT leniedijkshoorn diversityinthemajorpolysaccharideantigenofacinetobacterbaumanniiassessedbydnasequencinganddevelopmentofamolecularserotypingscheme AT leiwang diversityinthemajorpolysaccharideantigenofacinetobacterbaumanniiassessedbydnasequencinganddevelopmentofamolecularserotypingscheme AT peterrreeves diversityinthemajorpolysaccharideantigenofacinetobacterbaumanniiassessedbydnasequencinganddevelopmentofamolecularserotypingscheme
_version_	1718421009971806208

Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.

Ejemplares similares